Product Search for rat cd4 t cell: Novus Biologicals

NBP1-19371

CD4 Antibody - BSA Free

(9 Reviews)

(53 Publications)

0.1 ml

NBP1-19371

Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.

Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).

Flow Cytometry: CD4 Antibody - BSA Free [NBP1-19371] - An intracellular stain was performed on U-251 MG cells with CD4 Antibody NBP1-19371AF594 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 594.

Western Blot: CD4 Antibody [NBP1-19371] - Mouse spleen, 25ug total protein. Image from verified customer review.

Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Staining of paraffin-embedded mouse brain tissue. Photo courtesy of product review by verified customer.

Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Analysis of a FFPE tissue section of mouse brain using CD4 antibody at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody primarily generated a strong signal in the membranes of a subset of cells in the tested section.

Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Analysis of a FFPE section of mouse spleen using CD4 antibody at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a strong signal in the membranes of a subset of cells in the spleen section and the staining showed punctate appearance in some cells.

Immunohistochemistry-Frozen: CD4 Antibody [NBP1-19371] - Staining in mouse liver tissue.

Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Alcohol-fixed paraffin-embedded rat spleen tissue. Dilution 1:150. Image from verified customer review.

Flow Cytometry: CD4 Antibody [NBP1-19371] - An intracellular stain was performed on Jurkat cells with CD4 Antibody NBP1-19371R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.

Flow Cytometry: CD4 Antibody [NBP1-19371] - An intracellular stain was performed on Jurkat cells with CD4 Antibody NBP1-19371AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Flow Cytometry: CD4 Antibody [NBP1-19371] - An intracellular stain was performed on U937 cells with CD4 Antibody NBP1-19371AF594 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 594.

Flow Cytometry: CD4 Antibody [NBP1-19371] - An intracellular stain was performed on U937 cells with CD4 Antibody NBP1-19371G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.

Simple Western: CD4 Antibody [NBP1-19371] - Lane view shows a specific band for CD4 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Dual RNAscope ISH-IHC: CD4 Antibody [NBP1-19371] - IFNG mRNA (red) and CD4 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human lung cancer. ACD's Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-IFNG and CD4 antibody at 1:100 dilution. Tissue was stained on Leica Bond RX using RNAscope (TM) 2.5 LS Reagent Kit-RED, BOND Polymer Refine Detection (DAB) and Hematoxylin (Cat. No. DS9800), BOND Polymer Refine Red Detection and Hematoxylin and RNAscope (TM) 2.5 LS Green Accessory Pack. Tissue was counterstained with 50% hematoxylin (blue).

Dual RNAscope ISH-IHC: CD4 Antibody [NBP1-19371] - CCR5 mRNA (red) and CD4 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human lung cancer. ACD's Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-CCR5 and CD4 antibody at 1:100 dilution. Tissue was stained on Leica Bond RX using RNAscope (TM) 2.5 LS Reagent Kit-RED, BOND Polymer Refine Detection (DAB) and Hematoxylin, BOND Polymer Refine Red Detection and Hematoxylin and RNAscope (TM) 2.5 LS Green Accessory Pack. Tissue was counterstained with 50% hematoxylin (blue).

ATM activation by chloroquine alleviates senescence.(a) Immunoblots showing protein levels of ATM, NBS1, and RAP80 in human skin fibroblasts (HSFs). A gradually increased level of p16 indicates cellular senescence, while elevated gamma H2AX level indicates accumulated DNA damage. (b) Immunoblots showing protein levels of ATM, NBS1, and RAP80 in mouse embryonic fibroblasts (MEFs). (c) Immunoblots showing protein levels of ATM, NBS1, and RAP80 in brain tissues isolated from 3-, 10-, and 18-month-old male mice. (d) SA-beta -Gal staining in HSFs treated with sh-ATM or scramble shRNA. Scale bar, 100 µm. (e) Quantification of SA-beta -Gal-positive staining of (d) from five views randomly captured for each group. Data represent means ± SEM. ***p<0.001. (f) Immunoblots showing increased gamma H2AX and unaffected LC3I/II in HSFs treated with sh-ATM or scramble shRNA. (g) Immunoblots showing protein levels of pS1981 ATM, gamma H2AX, and cleaved caspase-3 in HSFs treated with 10 μM of CQ for indicated time. (h) SA-beta -Gal staining in HSFs expressing either scramble or ATM shRNA treated with 1 μM CQ or DMSO (12 hr). Scale bar, 100 µm. (i) Quantification of SA-beta -Gal-positive staining of (h) from five views randomly captured for each group. Data represent means ± SEM. ***p<0.001; ‘N.S.’ indicates no significant difference. (j) HSFs at passage 20 were continuously cultured with 1 μM CQ or DMSO, and cell number was calculated at each passage. Data represent means ± SEM. ***p<0.01. (k) Immunoblots showing protein levels of gamma H2AX, p62, and LC3 in MEFs treated with 1 μM CQ or DMSO. Note that CQ had little effect on the expression levels of p62 and LC3. (l) MEFs at passage one were continuously cultured in 20% O2 with 1 μM CQ or DMSO, and cell number was determined at each passage. Data represent means ± SEM. ***p<0.01.10.7554/eLife.34836.006Figure 1—source data 1.Statistical analysis for SA-beta -Gal positive staining.10.7554/eLife.34836.007Figure 1—source data 2.Statistical analysis for EdU positive staining.Sta

Immunocytochemistry/ Immunofluorescence: CD4 Antibody - BSA Free [NBP1-19371] - Rats with mild liver damage show immune cells infiltration in cerebellum at 4 weeks, which is prevented by rifaximin. Analysis of immune cells infiltration in cerebellum was performed at 4 weeks by immunohistochemistry using antibodies against (A) CD4, a marker of T lymphocytes (F(3,8) = 4.511, p < 0.01) & (B) IBA1, a marker of meningeal & perivascular macrophages (K-W(4,10) = 27.76, p < 0.0001). (C) Double immunofluorescence with anti-CD4 & anti-CX3CR1 as markers of autoreactive CD4+CD28− T lymphocytes was performed & quantified (K-W(4,5) = 8.711, p < 0.05). (D) Double immunofluorescence with anti-CD4 & anti-CCR6 as marker of Th17 lymphocytes was performed & quantified (K-W(4,7) = 8.858, p < 0.05). Number of animals in each group was added under group names. One-way ANOVA with Tukey’s test (CD4+) & nonparametric Kruskal–Wallis (K-W statistic) with Dunn’s test (Iba1, CD4+/CX3CR1+ & CD4+/CCR6+) was performed to compare all groups. Values significantly different from control rats are indicated by asterisks & from CCl4 rats by a. * p < 0.05, a p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34440206), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Topical 2% REB decreases the ocular inflammatory cytokines in PM-induced ocular surface inflammation in a rat model. (A) Immunofluorescence staining for (i) MMP-9, (ii) TNF-alpha , (iii) IL-1 beta , and (iv) IL-17 in the cornea of normal, PM-exposed, PM+REB, and PM+HA-treated groups. (B) Western blot analysis of pNF-kappa B and NF-kappa B, (C) IL-17, (D) CD-4, and (E) MMP-9 expression in the conjunctiva. (F) Changes in the expression of MMP-9, (G) TNF-alpha , (H) IL-1 beta , and (I) IL-17 in the cornea. (J) Relative expression of pNF-kappa B, (K) IL-17, (L) CD-4, and (M) MMP-9 normalized to GAPDH. In (F–M), the data are expressed as mean +/- SEM (n = 6, n = 4). One-way ANOVA was followed by the Tukey test. * p < 0.05, ** p < 0.01, *** p < 0.001 indicate statistically significant differences. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40332777), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immune signaling in the WAT during CoV-2 infection is altered differently between the sexes. Immunoblot images of (a) immune cell markers (CD4, CD8, F4/80) and (b) inflammatory markers (TNF alpha , IL-6, IL-10). beta -actin was used as loading control. Fold changes in the protein levels were normalized to beta -actin expression and are shown as bar graphs. The error bars represent standard error of the mean. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 compared to uninfected sex-matched mice and between the infected groups (n = 4/sex/group) (M, male; F, female). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36674830), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Rats with mild liver damage show immune cells infiltration in cerebellum at 4 weeks, which is prevented by rifaximin. Analysis of immune cells infiltration in cerebellum was performed at 4 weeks by immunohistochemistry using antibodies against (A) CD4, a marker of T lymphocytes (F(3,8) = 4.511, p < 0.01) and (B) IBA1, a marker of meningeal and perivascular macrophages (K-W(4,10) = 27.76, p < 0.0001). (C) Double immunofluorescence with anti-CD4 and anti-CX3CR1 as markers of autoreactive CD4+CD28− T lymphocytes was performed and quantified (K-W(4,5) = 8.711, p < 0.05). (D) Double immunofluorescence with anti-CD4 and anti-CCR6 as marker of Th17 lymphocytes was performed and quantified (K-W(4,7) = 8.858, p < 0.05). Number of animals in each group was added under group names. One-way ANOVA with Tukey’s test (CD4+) and nonparametric Kruskal–Wallis (K-W statistic) with Dunn’s test (Iba1, CD4+/CX3CR1+ and CD4+/CCR6+) was performed to compare all groups. Values significantly different from control rats are indicated by asterisks and from CCl4 rats by a. * p < 0.05, a p < 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34440206), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

(A) Immunofluorescence staining showing the expression of (i) PD-L1, (ii) CD4, (iii) CD3e and (iv) IL-17 localized in the corneas in the control, desiccation+PBS and desiccation+PD-L1 group. (B–E) Changes in the percentage of PD-L1, CD4, CD3e and IL-17 positive cells in the cornea. Immunopositivity for PD-L1 is shown in higher-power fields. Immunopositivity was counted in low-power fields and calculated as relative to the total number of DAPI-positive cells. (F,G) Western blot showing the expression of CD4 and IL-17 in the cornea. (H,I) Relative expression ratio of CD4 and IL-17 to beta -actin. In (B–E,H,I), data are presented with bar graph (n = 6, day 10, * p < 0.05; ** p < 0.01; *** p < 0.001 significant difference by one-way ANOVA). Original images of (F,G) can be found in Supplementary Materials. Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/2218-273X/14/1/68), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

RD and HFD differently regulate the expression levels of p-AMPK alpha , p-mTOR, p-Perilipin 1, CD4, CD8, F4/80, TNF alpha , IFN gamma , and IL-6 in tumor-associated mammary fat pad during early (1 wpi) and later (5 wpi) time points of tumor growth in young female E0771 tumor-bearing mice. (A) Immunoblots showing different protein expression between RD and HFD at 1 wpi and 5 wpi. beta -Actin was used as loading control. (B) Bar graphs showing the levels of each protein marker normalized to beta -Actin (top panel—1 wpi; bottom panel—5 wpi). The error bars represent the standard error of the mean. * p ≤ 0.05 and *** p ≤ 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36675341), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Ca, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Ca, Rb

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-19371AF350
Alexa Fluor 405	NBP1-19371AF405
Alexa Fluor 488	NBP1-19371AF488
Alexa Fluor 532	NBP1-19371AF532
Alexa Fluor 594	NBP1-19371AF594
Alexa Fluor 647	NBP1-19371AF647
Alexa Fluor 700	NBP1-19371AF700
Alexa Fluor 750	NBP1-19371AF750
Alexa Fluor Plus 405	NBP1-19371AFP405
Alexa Fluor Plus 488	NBP1-19371AFP488
Alexa Fluor Plus 555	NBP1-19371AFP555
Alexa Fluor Plus 594	NBP1-19371AFP594
Alexa Fluor Plus 647	NBP1-19371AFP647
Alexa Fluor Plus 680	NBP1-19371AFP680
Allophycocyanin	NBP1-19371APC
Biotin	NBP1-19371B
CoraFluor 1	NBP1-19371CL1
DyLight 350	NBP1-19371UV
DyLight 405	NBP1-19371V
DyLight 488	NBP1-19371G
DyLight 550	NBP1-19371R
DyLight 594	NBP1-19371DL594
DyLight 650	NBP1-19371C
DyLight 680	NBP1-19371FR
DyLight 755	NBP1-19371IR
FITC	NBP1-19371F
HRP	NBP1-19371H
Janelia Fluor 525	NBP1-19371JF525
Janelia Fluor 549	NBP1-19371JF549
Janelia Fluor 646	NBP1-19371JF646
Janelia Fluor 669	NBP1-19371JF669
PE	NBP1-19371PE
PerCP	NBP1-19371PCP
mFluor Violet 450 SE	NBP1-19371MFV450
mFluor Violet 500 SE	NBP1-19371MFV500
mFluor Violet 610 SE	NBP1-19371MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-19371SS

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AF6439

HAF016). A specific band was detected for CD4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of rat CD4<SUP>+</SUP>T cells and rat spleen tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Rat CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6439) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). A specific band was detected for CD4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of rat CD4⁺T cells and rat spleen tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Rat CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6439) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127)." class="big_lightbox">

Rat splenocytes were stained with Sheep Anti-Rat CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6439, filled histogram) or control antibody (Catalog # <a class=

5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127)." class="big_thumb" />

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

, ,

Applications: , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF6439U-100UG
Alexa Fluor 405	AF6439V-100UG
Alexa Fluor 488	AF6439G-100UG
Alexa Fluor 532	AF6439X-100UG
Alexa Fluor 594	AF6439T-100UG
Alexa Fluor 647	AF6439R-100UG
Alexa Fluor 700	AF6439N-100UG
Alexa Fluor 750	AF6439S-100UG
Alexa Fluor Plus 405	AF6439AFP405-100UG
Alexa Fluor Plus 488	AF6439AFP488-100UG
Alexa Fluor Plus 555	AF6439AFP555-100UG
Alexa Fluor Plus 594	AF6439AFP594-100UG
Alexa Fluor Plus 647	AF6439AFP647-100UG
Alexa Fluor Plus 680	AF6439AFP680-100UG
Allophycocyanin	AF6439A
Biotin	AF6439B
CoraFluor 1	AF6439CL1
DyLight 350	AF6439D
DyLight 405	AF6439E
DyLight 488	AF6439K
DyLight 550	AF6439L
DyLight 594	AF6439M
DyLight 650	AF6439W
DyLight 680	AF6439Y
DyLight 755	AF6439Z
FITC	AF6439F
HRP	AF6439H
Janelia Fluor 525	AF6439JF525
Janelia Fluor 549	AF6439I
Janelia Fluor 646	AF6439J
Janelia Fluor 669	AF6439JF669
PE	AF6439P
PerCP	AF6439C
mFluor Violet 450 SE	AF6439MFV450
mFluor Violet 500 SE	AF6439MFV500
mFluor Violet 610 SE	AF6439MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF6439-SP

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MAB6439

CD4 Antibody (743611)

(1 Publication)

100 μg

MAB6439

HAF018). A specific band was detected for CD4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of rat spleen tissue and rat CD4<SUP>+</SUP>T Cells. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Rat CD4 Monoclonal Antibody (Catalog # MAB6439) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF018). A specific band was detected for CD4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of rat spleen tissue and rat CD4⁺T Cells. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Rat CD4 Monoclonal Antibody (Catalog # MAB6439) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB6439U-100UG
Alexa Fluor 405	FAB6439V-100UG
Alexa Fluor 488	FAB6439G-100UG
Alexa Fluor 532	FAB6439X-100UG
Alexa Fluor 594	FAB6439T-100UG
Alexa Fluor 647	FAB6439R-100UG
Alexa Fluor 700	FAB6439N-100UG
Alexa Fluor 750	FAB6439S-100UG
Alexa Fluor Plus 405	FAB6439AFP405-100UG
Alexa Fluor Plus 488	FAB6439AFP488-100UG
Alexa Fluor Plus 555	FAB6439AFP555-100UG
Alexa Fluor Plus 594	FAB6439AFP594-100UG
Alexa Fluor Plus 647	FAB6439AFP647-100UG
Alexa Fluor Plus 680	FAB6439AFP680-100UG
Allophycocyanin	FAB6439A
Biotin	FAB6439B
CoraFluor 1	FAB6439CL1
DyLight 350	FAB6439D
DyLight 405	FAB6439E
DyLight 488	FAB6439K
DyLight 550	FAB6439L
DyLight 594	FAB6439M
DyLight 650	FAB6439W
DyLight 680	FAB6439Y
DyLight 755	FAB6439Z
FITC	FAB6439F
HRP	FAB6439H
Janelia Fluor 525	FAB6439JF525
Janelia Fluor 549	FAB6439I
Janelia Fluor 646	FAB6439J
Janelia Fluor 669	FAB6439JF669
PE	FAB6439P
PerCP	FAB6439C
mFluor Violet 450 SE	FAB6439MFV450
mFluor Violet 500 SE	FAB6439MFV500
mFluor Violet 610 SE	FAB6439MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB6439-SP

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MAB6676

CD4 Antibody (695542)

(1 Review)

100 μg

MAB6676

MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." class="big_lightbox">

MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." alt="Cotton rat splenocytes were stained with Mouse Anti-Cotton Rat CD4 Monoclonal Antibody (Catalog # MAB6676, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." class="big_thumb" />

Reactivity:

Cr

Species Glossary

Reactivity: Cr

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB6676U-100UG
Alexa Fluor 405	FAB6676V-100UG
Alexa Fluor 488	FAB6676G-100UG
Alexa Fluor 532	FAB6676X
Alexa Fluor 594	FAB6676T-100UG
Alexa Fluor 647	FAB6676R-100UG
Alexa Fluor 700	FAB6676N-100UG
Alexa Fluor 750	FAB6676S-100UG
Alexa Fluor Plus 405	FAB6676AFP405-100UG
Alexa Fluor Plus 488	FAB6676AFP488-100UG
Alexa Fluor Plus 555	FAB6676AFP555-100UG
Alexa Fluor Plus 594	FAB6676AFP594-100UG
Alexa Fluor Plus 647	FAB6676AFP647-100UG
Alexa Fluor Plus 680	FAB6676AFP680-100UG
Allophycocyanin/Cy7	FAB6676APCCY7
Biotin	FAB6676B
CoraFluor 1	FAB6676CL1
DyLight 350	FAB6676D
DyLight 405	FAB6676E
DyLight 488	FAB6676K
DyLight 550	FAB6676L
DyLight 594	FAB6676M
DyLight 650	FAB6676W
DyLight 680	FAB6676Y
DyLight 755	FAB6676Z
FITC	FAB6676F
HRP	FAB6676H
Janelia Fluor 525	FAB6676JF525
Janelia Fluor 549	FAB6676I
Janelia Fluor 646	FAB6676J
Janelia Fluor 669	FAB6676JF669
PE/Atto594	FAB6676PEATT594
PE/Cy5.5	FAB6676PECY55
PE/Cy7	FAB6676PECY7
PerCP	FAB6676C
mFluor Violet 450 SE	FAB6676MFV450
mFluor Violet 500 SE	FAB6676MFV500
mFluor Violet 610 SE	FAB6676MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB6676-SP
Carrier Free	FAB6676V-100UG
Carrier Free	FAB6676U-100UG
Carrier Free	FAB6676T-100UG
Carrier Free	FAB6676S-100UG
Carrier Free	FAB6676R-100UG
Carrier Free	FAB6676N-100UG
Carrier Free	FAB6676G-100UG

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AF6676

CD4 Antibody

100 μg

AF6676

5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127)." class="big_lightbox">

5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127)." alt="Cotton rat splenocytes were stained with Sheep Anti-Cotton Rat CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6676, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127)." class="big_thumb" />

Reactivity:

Cr

Species Glossary

Reactivity: Cr

Applications:

,

Applications: ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF6676U-100UG
Alexa Fluor 405	AF6676V-100UG
Alexa Fluor 488	AF6676G-100UG
Alexa Fluor 532	AF6676X-100UG
Alexa Fluor 594	AF6676T-100UG
Alexa Fluor 647	AF6676R-100UG
Alexa Fluor 700	AF6676N-100UG
Alexa Fluor 750	AF6676S-100UG
Alexa Fluor Plus 405	AF6676AFP405-100UG
Alexa Fluor Plus 488	AF6676AFP488-100UG
Alexa Fluor Plus 555	AF6676AFP555-100UG
Alexa Fluor Plus 594	AF6676AFP594-100UG
Alexa Fluor Plus 647	AF6676AFP647-100UG
Alexa Fluor Plus 680	AF6676AFP680-100UG
Allophycocyanin	AF6676A
Biotin	AF6676B
CoraFluor 1	AF6676CL1
DyLight 350	AF6676D
DyLight 405	AF6676E
DyLight 488	AF6676K
DyLight 550	AF6676L
DyLight 594	AF6676M
DyLight 650	AF6676W
DyLight 680	AF6676Y
DyLight 755	AF6676Z
FITC	AF6676F
HRP	AF6676H
Janelia Fluor 525	AF6676JF525
Janelia Fluor 549	AF6676I
Janelia Fluor 646	AF6676J
Janelia Fluor 669	AF6676JF669
PE	AF6676P
PerCP	AF6676C
mFluor Violet 450 SE	AF6676MFV450
mFluor Violet 500 SE	AF6676MFV500
mFluor Violet 610 SE	AF6676MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF6676-SP

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MAB5541

CD4 Antibody (131922)

(1 Publication)

500 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB5541U-100UG
Alexa Fluor 405	FAB5541V-100UG
Alexa Fluor 488	FAB5541G-100UG
Alexa Fluor 532	FAB5541X-100UG
Alexa Fluor 594	FAB5541T-100UG
Alexa Fluor 647	FAB5541R-100UG
Alexa Fluor 700	FAB5541N-100UG
Alexa Fluor 750	FAB5541S-100UG
Alexa Fluor Plus 405	FAB5541AFP405-100UG
Alexa Fluor Plus 488	FAB5541AFP488-100UG
Alexa Fluor Plus 555	FAB5541AFP555-100UG
Alexa Fluor Plus 594	FAB5541AFP594-100UG
Alexa Fluor Plus 647	FAB5541AFP647-100UG
Alexa Fluor Plus 680	FAB5541AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB5541-SP

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NB100-65351

CD4 Antibody (OX-38) - BSA Free

0.25 mg

NB100-65351

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	NB100-65351B-0.25ml
CoraFluor 1	NB100-65351CL1
DyLight 350	NB100-65351UV-0.25ml
DyLight 405	NB100-65351V-0.25ml
DyLight 488	NB100-65351G-0.25ml
DyLight 550	NB100-65351R-0.25ml
DyLight 650	NB100-65351C-0.25ml
DyLight 680	NB100-65351FR-0.25ml
DyLight 755	NB100-65351IR-0.25ml
FITC	NB100-65351F-0.25ml
HRP	NB100-65351H-0.25ml
Janelia Fluor 525	NB100-65351JF525
Janelia Fluor 669	NB100-65351JF669
PE	NB100-65351PE-0.25ml
mFluor Violet 450 SE	NB100-65351MFV450
mFluor Violet 500 SE	NB100-65351MFV500
mFluor Violet 610 SE	NB100-65351MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-65351SS
Azide and BSA Free	NBP2-80648

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6439-CD

Recombinant Rat sCD4 Protein, CF

50 μg

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

Applications:

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6676-CD

Recombinant Cotton Rat sCD4 Protein, CF

50 μg

Reactivity:

Cr

Species Glossary

Reactivity: Cr

Applications:

Applications:

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NBP2-25191

CD4 Antibody (GK1.5) - BSA Free

(7 Publications)

0.1 mg

NBP2-25191

Flow Cytometry: CD4 Antibody (GK1.5) [NBP2-25191] - Analysis using the Allophycocyanin conjugate of NBP2-26585. Staining of CD4 in 10^6 BALB/c mouse splenocytes using 1 ug of this antibody. Green represents isotype control; red represents mouse CD4 this antibody. Image using the Azide Free format of this antibody.

Flow Cytometry: CD4 Antibody (GK1.5) [NBP2-25191] - Analysis using the FITC conjugate of NBP2-26585. Staining of CD4 in 10^6 BALB/c mouse splenocytes using 1 ug of NBP2-26584. Green represents isotype control (20212C); red represents mouse CD4 NBP2-26584 antibody. Image using the Azide Free format of this antibody.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Alt Catalog#

IMG-5922A

Alt Catalog# IMG-5922A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-25191AF350
Alexa Fluor 405	NBP2-25191AF405
Alexa Fluor 488	NBP2-25191AF488
Alexa Fluor 532	NBP2-25191AF532
Alexa Fluor 594	NBP2-25191AF594
Alexa Fluor 647	NBP2-25191AF647
Alexa Fluor 700	NBP2-25191AF700
Alexa Fluor 750	NBP2-25191AF750
Alexa Fluor Plus 405	NBP2-25191AFP405
Alexa Fluor Plus 488	NBP2-25191AFP488
Alexa Fluor Plus 555	NBP2-25191AFP555
Alexa Fluor Plus 594	NBP2-25191AFP594
Alexa Fluor Plus 647	NBP2-25191AFP647
Alexa Fluor Plus 680	NBP2-25191AFP680
Allophycocyanin	NBP2-26598
Allophycocyanin/Cy7	NBP2-25191APCCY7
Biotin	NBP2-25191B
CoraFluor 1	NBP2-25191CL1
DyLight 350	NBP2-25191UV
DyLight 405	NBP2-25191V
DyLight 488	NBP2-25191G
DyLight 550	NBP2-25191R
DyLight 594	NBP2-25191DL594
DyLight 650	NBP2-25191C
DyLight 680	NBP2-25191FR
DyLight 755	NBP2-25191IR
FITC	NBP2-25191F
HRP	NBP2-25191H
Janelia Fluor 525	NBP2-25191JF525
Janelia Fluor 549	NBP2-25191JF549
Janelia Fluor 646	NBP2-25191JF646
Janelia Fluor 669	NBP2-25191JF669
PE	NBP2-25191PE
PE/Atto594	NBP2-25191PEATT594
PE/Cy5.5	NBP2-25191PECY55
PE/Cy7	NBP2-25191PECY7
PerCP	NBP2-25191PCP
mFluor Violet 450 SE	NBP2-25191MFV450
mFluor Violet 500 SE	NBP2-25191MFV500
mFluor Violet 610 SE	NBP2-25191MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-26585
BSA Free	NBP2-25191SS
Azide and BSA Free	NBP2-26585-1mg
Azide and BSA Free	NBP2-26585-25ug

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FAB65771T

CD4 Antibody (OX38) [Alexa Fluor® 594]

100 μg

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Alexa Fluor 594

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB65771U-100UG
Alexa Fluor 405	FAB65771V-100UG
Alexa Fluor 488	FAB65771G-100UG
Alexa Fluor 532	FAB65771X
Alexa Fluor 647	FAB65771R-100UG
Alexa Fluor 700	FAB65771N-100UG
Alexa Fluor 750	FAB65771S-100UG
Alexa Fluor Plus 405	FAB65771AFP405-100UG
Alexa Fluor Plus 488	FAB65771AFP488-100UG
Alexa Fluor Plus 555	FAB65771AFP555-100UG
Alexa Fluor Plus 594	FAB65771AFP594-100UG
Alexa Fluor Plus 647	FAB65771AFP647-100UG
Alexa Fluor Plus 680	FAB65771AFP680-100UG
Allophycocyanin	FAB65771A
Allophycocyanin/Cy7	FAB65771APCCY7
Biotin	FAB65771B
CoraFluor 1	FAB65771CL1
DyLight 350	FAB65771D
DyLight 405	FAB65771E
DyLight 488	FAB65771K
DyLight 550	FAB65771L
DyLight 594	FAB65771M
DyLight 650	FAB65771W
DyLight 680	FAB65771Y
DyLight 755	FAB65771Z
FITC	FAB65771F
HRP	FAB65771H
Janelia Fluor 525	FAB65771JF525
Janelia Fluor 549	FAB65771I
Janelia Fluor 646	FAB65771J
Janelia Fluor 669	FAB65771JF669
PE	FAB65771P
PE/Atto594	FAB65771PEATT594
PE/Cy5.5	FAB65771PECY55
PE/Cy7	FAB65771PECY7
PerCP	FAB65771C
mFluor Violet 450 SE	FAB65771MFV450
mFluor Violet 500 SE	FAB65771MFV500
mFluor Violet 610 SE	FAB65771MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB65771G-100UG
Carrier Free	FAB65771N-100UG
Carrier Free	FAB65771U-100UG
Carrier Free	FAB65771V-100UG
Carrier Free	FAB65771R-100UG
Carrier Free	FAB65771S-100UG

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FAB554C

CD4 Antibody (GK1.5) [PerCP (Peridinin-chlorophyll Protein Complex)]

100 Tests

FAB554C

FAB4841G) and either (A) Rat Anti-Mouse CD4 PerCP-conjugated Monoclonal Antibody (Catalog # FAB554C) or (B) Rat IgG_2BPerCP Isotype Control (Catalog # IC013C). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

Mouse splenocytes were stained with Rat Anti-Mouse CD3 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # <a class=

FAB4841G) and either (A) Rat Anti-Mouse CD4 PerCP-conjugated Monoclonal Antibody (Catalog # FAB554C) or (B) Rat IgG_2BPerCP Isotype Control (Catalog # IC013C). View our protocol for Staining Membrane-associated Proteins." alt="Mouse splenocytes were stained with Rat Anti-Mouse CD3 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB4841G) and either (A) Rat Anti-Mouse CD4 PerCP-conjugated Monoclonal Antibody (Catalog # FAB554C) or (B) Rat IgG_2BPerCP Isotype Control (Catalog # IC013C). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Conjugate:

PerCP (Peridinin-chlorophyll Protein Complex)

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB554U-100UG
Alexa Fluor 405	FAB554V-100UG
Alexa Fluor 488	FAB554G-025
Alexa Fluor 488	FAB554G-100
Alexa Fluor 532	FAB554X
Alexa Fluor 750	FAB554S-025
Alexa Fluor 750	FAB554S-100
Alexa Fluor Plus 405	FAB554AFP405-100UG
Alexa Fluor Plus 488	FAB554AFP488-100UG
Alexa Fluor Plus 555	FAB554AFP555-100UG
Alexa Fluor Plus 594	FAB554AFP594-100UG
Alexa Fluor Plus 647	FAB554AFP647-100UG
Alexa Fluor Plus 680	FAB554AFP680-100UG
Allophycocyanin	FAB554A-025
Allophycocyanin	FAB554A-100
Allophycocyanin/Cy7	FAB554APCCY7
CoraFluor 1	FAB554CL1
DyLight 350	FAB554D
DyLight 405	FAB554E
DyLight 488	FAB554K
DyLight 550	FAB554L
DyLight 594	FAB554M
DyLight 650	FAB554W
DyLight 680	FAB554Y
DyLight 755	FAB554Z
Fluorescein	FAB554F-100
Fluorescein	FAB554F-025
HRP	FAB554H
Janelia Fluor 525	FAB554JF525
Janelia Fluor 549	FAB554I
Janelia Fluor 646	FAB554J
Janelia Fluor 669	FAB554JF669
PE/Atto594	FAB554PEATT594
PE/Cy5.5	FAB554PECY55
PE/Cy7	FAB554PECY7
PerCP (Peridinin-chlorophyll Protein Complex)	FAB554C-025
Phycoerythrin	FAB554P-100
Phycoerythrin	FAB554P-025
mFluor Violet 450 SE	FAB554MFV450
mFluor Violet 500 SE	FAB554MFV500
mFluor Violet 610 SE	FAB554MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB554V-100UG
Carrier Free	FAB554F-100
Carrier Free	FAB554P-100
Carrier Free	FAB554A-100
Carrier Free	FAB554G-100
Carrier Free	FAB554A-025
Carrier Free	FAB554C-025
Carrier Free	FAB554F-025
Carrier Free	FAB554G-025
Carrier Free	FAB554P-025
Carrier Free	FAB554S-100
Carrier Free	FAB554S-025
Carrier Free	FAB554U-100UG

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

CD4 Antibody - BSA Free

CD4 Antibody

CD4 Antibody (743611)

CD4 Antibody (695542)

CD4 Antibody

CD4 Antibody (131922)

CD4 Antibody (OX-38) - BSA Free

Recombinant Rat sCD4 Protein, CF

Recombinant Cotton Rat sCD4 Protein, CF

CD4 Antibody (GK1.5) - BSA Free

CD4 Antibody (OX38) [Alexa Fluor® 594]

CD4 Antibody (GK1.5) [PerCP (Peridinin-chlorophyll Protein Complex)]