| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33122-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-45325 | |||
| Azide and BSA Free | NBP2-34588-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-25195SS | |||
| Azide and BSA Free | NBP2-80659 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-24147 | |||
MAB0041) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). View our protocol for Staining Membrane-associated Proteins." alt="Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CD8 alpha Monoclonal Antibody (Catalog # MAB1509) or (B) Mouse IgG2B isotype control antibody (Catalog # MAB0041) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64922-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-45323 | |||
| Azide and BSA Free | NBP2-34589-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-45326 | |||
| Azide and BSA Free | NBP2-34590-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65729SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-45324 | |||
| Azide and BSA Free | NBP2-34790-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide Free | NB100-65928-0.025mg | |||
![Immunohistochemistry-Frozen: CD8 Antibody [NBP2-29475] - Mouse Ccdc88b expression in colon during DSS-induced colitis. Wild type (WT) mice were either not treated (NT) or given 3% DSS for 5 days followed by 3 days of water. aCcdc88b mRNA expression in distal colons of NT (n = 3) or DSS-treated WT mice (n = 3 for each time point) at the indicated days. Data represent expression relative to hprt±SEM (n = 3). *P < 0.05, **P < 0.01 (two-tailed Student’s t-test) & are representative of one experiment. b Representative immunoblots for Ccdc88b protein detected in extracts from distal colons from NT or DSS-treated WT & Ccdc88Bmut mutant mice at indicated time points, & densitometric quantification of Ccdc88b immunoblot normalized to beta -actin±SEM (n = 3) (representative of one of two independent experiments); **P < 0.01 (two-tailed Student’s t-test). c Representative confocal microscopy images of tissue sections from colon of NT or DSS-treated mice at indicated times, & stained with antibodies against Ccdc88b (red), CD45 (green), & E-Cadherin (purple) & nuclei staining DAPI (blue). Scale bars, 100 μm. d Representative FACS plots & quantification of lamina propria cells stained for CD45 & for Ccdc88b antibodies for NT (n = 3) & DSS-treated WT mice (n = 4 for each time point) at indicated time points, data are representative of one experiment. e Representative confocal microscopy images of tissue sections from colon of NT or DSS-treated mice at day 8, & stained for Ccdc88b (red), CD3 (green) & CD11b (green); nuclei are stained with DAPI (blue). Scale bars, 100 μm. f same as in e for Ccdc88b (red), CD4 (green) & CD8 (green). Scale bars, 100 μm Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-017-01381-y), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-29475_rabbit-polyclonal-cd8-antibody-imgenex-abx-160a-20ug-310202416113534.jpg)
![Immunohistochemistry-Paraffin: CD8 Antibody (C8/144B) [NBP2-32836] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD8 Antibody (C8/144B).](http://images.novusbio.com/fullsize/CD8-Antibody-C8-144B-Immunohistochemistry-Paraffin-NBP2-32836-img0003.jpg "Immunohistochemistry-Paraffin: CD8 Antibody (C8/144B) [NBP2-32836] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD8 Antibody (C8/144B).")
![Immunohistochemistry-Paraffin: CD8 Antibody (C8/144B) [NBP2-32836] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD8 Antibody (C8/144B).](http://images.novusbio.com/fullsize/CD8-Antibody-C8-144B-Immunohistochemistry-Paraffin-NBP2-32836-img0002.jpg)
![Flow Cytometry: CD8 Antibody (RPA-T8) [NBP2-25195] - CD8 alpha Antibody (RPA-T8) [NBP2-25195] - Analysis using PerCP conjugate of NBP2-25195. A cell surface stain was performed on hPBMCs with CD8 Alpha antibody (RPA-T8) NBP2-25195 (top image) and a matched isotype control NBP2-27287 (bottom image). Cells were incubated in an antibody dilution of 1:200 for 20 minutes at room temperature. Both antibodies were conjugated to PerCP. A co-stain was also performed using CD3 antibody NBP2-24867AF488.](http://images.novusbio.com/fullsize/CD8-Antibody-RPA-T8-Flow-Cytometry-NBP2-25195-img0015.jpg "Flow Cytometry: CD8 Antibody (RPA-T8) [NBP2-25195] - CD8 alpha Antibody (RPA-T8) [NBP2-25195] - Analysis using PerCP conjugate of NBP2-25195. A cell surface stain was performed on hPBMCs with CD8 Alpha antibody (RPA-T8) NBP2-25195 (top image) and a matched isotype control NBP2-27287 (bottom image). Cells were incubated in an antibody dilution of 1:200 for 20 minutes at room temperature. Both antibodies were conjugated to PerCP. A co-stain was also performed using CD3 antibody NBP2-24867AF488.")
![Flow Cytometry: CD8 Antibody (RPA-T8) [NBP2-25195] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-25195. Staining of CD8 alpha in human PBMC using CD8 alpha antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/CD8-Antibody-RPA-T8-Flow-Cytometry-NBP2-25195-img0012.jpg)
![Flow Cytometry: CD8 Antibody (RPA-T8) [NBP2-25195] - Analysis using the DyLight 405 conjugate of NBP2-25195. Staining of CD8 alpha in human PBMCs using anti-CD8 alpha antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/CD8-Antibody-RPA-T8-Flow-Cytometry-NBP2-25195-img0013.jpg)
![Flow (Cell Surface): CD8 Antibody (RPA-T8) [NBP2-25195] - CD8 alpha Antibody (RPA-T8) [NBP2-25195] - Analysis using the PE conjugate of NBP2-25195. Staining of Human peripheral blood mononuclear cells. Image from verified customer review.](http://images.novusbio.com/fullsize/CD8-Antibody-RPA-T8-Flow-Cell-Surface-NBP2-25195-img0014.jpg)
![Flow Cytometry: CD8 Antibody (RPA-T8) - Azide and BSA Free [NBP2-25195] - Analysis using the PE conjugate of NBP2-25195. Staining of CD8 in 10^6 human PBMC using 10 ul (0.1 ug) of this antibody. The shaded histogram represents human PBMC alone; green represents mouse isotype control, red represents human CD8 this antibody.](http://images.novusbio.com/fullsize/nbp2-25195_mouse-monoclonal-cd8-antibody-rpa-t8-imgenex-img-5917a-25ug-1772024104463.jpg)
![Flow Cytometry: CD8 Antibody (RPA-T8) - Azide and BSA Free [NBP2-25195] - Analysis using the FITC conjugate of NBP2-25195. Staining of CD8 in human PBMCs using 0.25 ug of (clone RPA-T8) per approximately 1x10^6 cells. Green represents isotype control this antibody, red represents anti-CD8 FITC antibody.](http://images.novusbio.com/fullsize/nbp2-25195_mouse-monoclonal-cd8-antibody-rpa-t8-imgenex-img-5917a-25ug-17720241044620.jpg)
NL009) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for ![Flow Cytometry: CD8 alpha Antibody (YTC182.20) [NB100-64922] - Using the Alexa Fluor 647 direct conjugate A surface stain was performed on human peripheral blood lymphocytes with CD8 alpha (YTC128.20) antibody NB100-64922AF647 and a matched isotype control NBP2-21941AF647. Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-24867AF488.](http://images.novusbio.com/fullsize/CD8-alpha-Antibody-YTC182-20-Flow-Cytometry-NB100-64922-img0003.jpg "Flow Cytometry: CD8 alpha Antibody (YTC182.20) [NB100-64922] - Using the Alexa Fluor 647 direct conjugate A surface stain was performed on human peripheral blood lymphocytes with CD8 alpha (YTC128.20) antibody NB100-64922AF647 and a matched isotype control NBP2-21941AF647. Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-24867AF488.")
![Flow Cytometry: CD8 alpha Antibody (YTC182.20) [NB100-64922] - Staining of human peripheral blood lymphocytes.](http://images.novusbio.com/fullsize/CD8-alpha-Antibody-YTC182-20-Flow-Cytometry-NB100-64922-img0001.jpg)
![Flow Cytometry: CD8 alpha Antibody (YTC182.20) [NB100-64922] - Staining of human peripheral blood lymphocytes using CD8 alpha FITC.](http://images.novusbio.com/fullsize/CD8-alpha-Antibody-YTC182-20-Flow-Cytometry-NB100-64922-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD8 Antibody (C8/468) [NBP2-32952] - Immunofluorescence staining of human PBMC cells using CD8 Mouse Monoclonal Antibody (C8/468) followed by goat anti-mouse IgG-CF488 (green).](http://images.novusbio.com/fullsize/CD8-Antibody-C8-468-Immunocytochemistry-Immunofluorescence-NBP2-32952-img0002.jpg "Immunocytochemistry/Immunofluorescence: CD8 Antibody (C8/468) [NBP2-32952] - Immunofluorescence staining of human PBMC cells using CD8 Mouse Monoclonal Antibody (C8/468) followed by goat anti-mouse IgG-CF488 (green).")
![Immunohistochemistry-Paraffin: CD8 Antibody (C8/468) [NBP2-32952] - Formalin-fixed, paraffin-embedded human tonsil stained with CD8 Mouse Monoclonal Antibody (C8/468).](http://images.novusbio.com/fullsize/CD8-Antibody-C8-468-Immunohistochemistry-Paraffin-NBP2-32952-img0003.jpg)
![Immunohistochemistry-Paraffin: CD8 Antibody (C8/468) [NBP2-32952] - Formalin-fixed, paraffin-embedded human lymph node stained with CD8 Mouse Monoclonal Antibody (C8/468).](http://images.novusbio.com/fullsize/CD8-Antibody-C8-468-Immunohistochemistry-Paraffin-NBP2-32952-img0004.jpg)
![Immunohistochemistry-Paraffin: CD8 Antibody (C8/468 + C8/144B) [NBP2-34318] - Formalin-fixed paraffin-embedded human tonsil stained with CD8 Monoclonal Antibody (C8/468+C8/144)](http://images.novusbio.com/fullsize/CD8-Antibody-C8-468-+-C8-144B-Immunohistochemistry-Paraffin-NBP2-34318-img0001.jpg "Immunohistochemistry-Paraffin: CD8 Antibody (C8/468 + C8/144B) [NBP2-34318] - Formalin-fixed paraffin-embedded human tonsil stained with CD8 Monoclonal Antibody (C8/468+C8/144)")
![Dual RNAscope ISH-IHC: CD8 Antibody (4B11) [NB100-65729] - Formalin-fixed paraffin-embedded tissue sections of human tonsil were probed for CD8 mRNA (ACD RNAScope probe, catalog # 560391; Fast Red chromogen, ACD catalog # 322500) followed by immunohistochemistry using mouse monoclonal (Novus catalog # NB100-65729) at 5ug/mL for 1 hour at room temperature and detection with anti-mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems Catalog # VC001) and green HRP chromogen (ACD Catalog # 322500).](http://images.novusbio.com/fullsize/CD8-Antibody-4B11-Dual-RNAscope-ISH-IHC-NB100-65729-img0003.jpg "<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: CD8 Antibody (4B11) [NB100-65729] - Formalin-fixed paraffin-embedded tissue sections of human tonsil were probed for CD8 mRNA (ACD RNAScope probe, catalog # 560391; Fast Red chromogen, ACD catalog # 322500) followed by immunohistochemistry using mouse monoclonal (Novus catalog # NB100-65729) at 5ug/mL for 1 hour at room temperature and detection with anti-mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems Catalog # VC001) and green HRP chromogen (ACD Catalog # 322500).")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD8 Antibody (4B11) [NB100-65729] - Analysis of formalin fixed, paraffin-embedded human tonsil with CD8 Antibody (4B11). Magnification: 40X.](http://images.novusbio.com/fullsize/CD8-alpha-Antibody-4B11-Immunohistochemistry-Paraffin-NB100-65729-img0002.jpg)
![Immunohistochemistry-Paraffin: CD8 Antibody (SPM548) [NBP2-32835] - Formalin-fixed, paraffin-embedded human tonsil (20X) stained with CD8 Ab (SPM548). Note Cell surface staining.](http://images.novusbio.com/fullsize/CD8-Antibody-SPM548-Immunohistochemistry-Paraffin-NBP2-32835-img0002.jpg "Immunohistochemistry-Paraffin: CD8 Antibody (SPM548) [NBP2-32835] - Formalin-fixed, paraffin-embedded human tonsil (20X) stained with CD8 Ab (SPM548). Note Cell surface staining.")
![Western Blot: CD8 Antibody (BLR044F) [NBP2-76422] - Detection of human CD8 by WB of CD4+ T-cells, CD8+ T-cells, Jurkat, MOLT-4, RPMI-8226, MJ, C5/MJ, KG-1, SR, and K-562 cells. Antibody: Rabbit anti-CD8 alpha recombinant monoclonal [BLR044F] (NBP2-76422). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower panel: Recombinant monoclonal antibodies to CD3e (NBP2-76424) and CD4 (NBP2-76423). A band appears at an observed molecular weight of approximately 32 kDa for the CD8 analysis.](http://images.novusbio.com/fullsize/CD8-Antibody-BLR044F-Western-Blot-NBP2-76422-img0005.jpg "Western Blot: CD8 Antibody (BLR044F) [NBP2-76422] - Detection of human CD8 by WB of CD4+ T-cells, CD8+ T-cells, Jurkat, MOLT-4, RPMI-8226, MJ, C5/MJ, KG-1, SR, and K-562 cells. Antibody: Rabbit anti-CD8 alpha recombinant monoclonal [BLR044F] (NBP2-76422). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower panel: Recombinant monoclonal antibodies to CD3e (NBP2-76424) and CD4 (NBP2-76423). A band appears at an observed molecular weight of approximately 32 kDa for the CD8 analysis.")
![Immunocytochemistry/Immunofluorescence: CD8 Antibody (BLR044F) [NBP2-76422] - Multiplex immunofluorescence analysis using rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] , rabbit anti-CD8 alpha recombinant monoclonal [BLR044F], rabbit anti-PD-L1 recombinant monoclonal [BLR020E]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™ 520, 620, and 690. Counterstain: DAPI (blue).](http://images.novusbio.com/fullsize/CD8-Antibody-BLR044F-Immunocytochemistry-Immunofluorescence-NBP2-76422-img0011.jpg)
![Immunohistochemistry-Paraffin: CD8 Antibody (BLR044F) [NBP2-76422] - Detection of human CD8 alpha in FFPE tonsil by IHC. Antibody: Rabbit anti-CD8 alpha recombinant monoclonal [BLR044F] (NBP2-76422). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CD8-Antibody-BLR044F-Immunohistochemistry-NBP2-76422-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD8 Antibody (BLR044F) [NBP2-76422] - Detection of human CD8 alpha (red) in FFPE melanoma by IHC-IF. Antibody: Rabbit anti-CD8 alpha recombinant monoclonal [BLR044F] NBP2-76422. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/CD8-Antibody-BLR044F-Immunocytochemistry-Immunofluorescence-NBP2-76422-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD8 Antibody (BLR044F) [NBP2-76422] - Detection of human CD8 alpha (red) in FFPE breast carcinoma by IHC-IF. Antibody: Rabbit anti-CD8 alpha recombinant monoclonal [BLR044F]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).](http://images.novusbio.com/fullsize/CD8-Antibody-BLR044F-Immunocytochemistry-Immunofluorescence-NBP2-76422-img0003.jpg)
