| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77330-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3984-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-04830-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF7646-SP | |||
![Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - YM155 significantly reduced tumorsphere formation and inhibited EGFR autophosphorylation and G9a expression. YM155 is reported to suppress cancer stemness; therefore, we used this compound to investigate the cellular mechanism of tumorsphere formation. To compare the cytotoxic capacity of YM155 against tumorsphere formation and parental cell lines, YM155 was applied to HCC827 and A549 cells in the stemness cultured or integrated medium. (F) Moreover, YM155 inhibited EGFR autophosphorylation in A549-derived tumorspheres and reduced Oct4 expression. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0182149) licensed under a CC-BY license. OCT4 Antibody [NB100-2379]](http://images.novusbio.com/fullsize/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-NBP1-42823-img0016.jpg)
![Western Blot: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - Detection of SOX2 in mouse brain lysate using NB110-37235 (0.5ug/ml).](http://images.novusbio.com/fullsize/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-Western-Blot-NBP1-42823-img0013.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - Nanog-inducible mouse model. Representative hematoxylin and eosin (H&E) staining and immunohistochemistry (IHC) for NANOG, Ki67 and LORICRIN of TPA-treated CTR and TG mice (bars correspond to 50 um). Statistical significance was determined by the two-tailed Student's t test: (*) p < 0.05; (**) p < 0.01. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep10205), licensed under a CC-BY license. Nanog Antibody [NB100-58842]](http://images.novusbio.com/fullsize/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-Immunohistochemistry-NBP1-42823-img0015.jpg)
![Western Blot: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - Whole cell lysate (5, 15, and 50 ug) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nanog antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds](http://images.novusbio.com/fullsize/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-Western-Blot-NBP1-42823-img0011.jpg)
![Immunohistochemistry: Pluripotent Stem Cell Transcription Factor Antibody Pack [NBP1-42823] - SOX2 staining (NB110-37235) of human uterus, endometrial glands.](http://images.novusbio.com/fullsize/Pluripotent-Stem-Cell-Transcription-Factor-Antibody-Pack-Immunohistochemistry-NBP1-42823-img0017.jpg)
![Western Blot: Borealin Antibody [NBP1-77330] - Rat kidney lysate with Borealin antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/Borealin-Antibody-Western-Blot-NBP1-77330-img0005.jpg "Western Blot: Borealin Antibody [NBP1-77330] - Rat kidney lysate with Borealin antibody at (A) 1 and (B) 2 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: Borealin Antibody - BSA Free [NBP1-77330] - Immunofluorescence of Borealin in Rat Kidney cells with Borealin antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77330_rabbit-polyclonal-borealin-antibody-2711202419353815.jpg)
![Western Blot: Dppa5 Overexpression Lysate (Adult Normal) [NBL1-10004] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Dppa5.](http://images.novusbio.com/fullsize/Dppa5-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10004-img0002.jpg "Western Blot: Dppa5 Overexpression Lysate (Adult Normal) [NBL1-10004] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Dppa5.")
![Western Blot: FOPNL Overexpression Lysate (Adult Normal) [NBL1-08223] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FOPNL.](http://images.novusbio.com/fullsize/FOPNL-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-08223-img0002.jpg "Western Blot: FOPNL Overexpression Lysate (Adult Normal) [NBL1-08223] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FOPNL.")
![Western Blot: FOPNL Antibody [NBP3-04830] - Analysis of extracts of Mouse testis, using FOPNL antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit .](http://images.novusbio.com/fullsize/FOPNL-Antibody-Western-Blot-NBP3-04830-img0001.jpg "Western Blot: FOPNL Antibody [NBP3-04830] - Analysis of extracts of Mouse testis, using FOPNL antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit .")
![Immunohistochemistry-Paraffin: DNMT3B Antibody [NB300-516] - Analysis showing positive staining in the nucleus and cytoplasm of Human breast carcinoma (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize/DNMT3B-Antibody-Immunohistochemistry-Paraffin-NB300-516-img0010.jpg)
![Western Blot: DNMT3B Antibody [NB300-516] - Homogenized mouse brain cells. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/DNMT3B-Antibody-Western-Blot-NB300-516-img0006.jpg)
![Immunohistochemistry-Paraffin: DNMT3B Antibody [NB300-516] - Analysis showing positive staining in the nucleus of Human testis tissue (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize/DNMT3B-Antibody-Immunohistochemistry-Paraffin-NB300-516-img0008.jpg)
![Immunohistochemistry-Paraffin: DNMT3B Antibody [NB300-516] - Analysis showing positive staining in the nucleus and cytoplasm of Mouse brain tissue (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize/DNMT3B-Antibody-Immunohistochemistry-Paraffin-NB300-516-img0009.jpg)
![Simple Western: DNMT3B Antibody [NB300-516] - Simple Western lane view shows a specific band for DNMT3B in 0.5 mg/mL of BG01V lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody](http://images.novusbio.com/fullsize/DNMT3B-Antibody-Simple-Western-NB300-516-img0007.jpg)
![Simple Western: DNMT3B Antibody [NB300-516] - Analysis of 25 ug of HeLa cell lysates and a molecular weight protein ladder.](http://images.novusbio.com/fullsize/DNMT3B-Antibody-Simple-Western-NB300-516-img0011.jpg)
![Western Blot: DNMT3B Antibody [NB300-516] - DNMT3B is a direct target of miR-30a & miR-379. a The effect of miRNA mimics (PM-30a or PM-379, 20 nM) on the luciferase activities of the constructs containing the wild-type (wt-3′-UTR) or mutant-type 3′-UTR (mt-30a-3′-UTR or mt-379-3′-UTR) in OEC-M1 cells. The relative luciferase activity of each sample is measured at 48 h after transfection & normalized to Renilla luciferase activity. The data are represented as mean ± SD; ***p < 0.001 versus control mimics (PM-NC). b RT-PCR & Western blot analysis of DNMT3B level in SCC-15 & OEC-M1 cells after treatment with control mimics (NC), or miRNA mimics (PM-30a or PM-379, 20 nM) or miRNA inhibitor (AM-30a or AM-379, 20 nM) for 48 h. GAPDH & alpha -tubulin were used as internal control, respectively. c RT-PCR analysis of ADHFE1 & ALDH1A2 expression level after treatment with control mimics (NC, 20 nM), or miRNA mimics (PM-30a or PM-379, 20 nM) for 48 h in SCC-15 & OEC-M1 cells. GAPDH was used as internal control. d Relative miR-30a & miR-379 expression levels in 33 of adjacent normal tissues (N) compared with their own tumors (T). e Correlation analysis of DNMT3B & miR-30a or miR-379 in OSCC patients (n = 33) by qRT-PCR analysis. Pearson correlation coefficients & p-values were calculated as indicated. Red, tumor part; green, normal part Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32238162), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-516_rabbit-polyclonal-dnmt3b-antibody-310202416205125.jpg)
![Western Blot: DNMT3B Antibody [NB300-516] - Arecoline & NNK induced DNMT3B activity & repressed ADHFE1, ALDH1A2 & miRNAs expression. a qRT–PCR analysis of miR-30a & miR-379 expression level after treatment with arecoline (50 μM) or NNK (10 μM) for indicated days. b RT-PCR analysis of ADHFE1, ALDH1A2 level & western blot analysis of DNMT3B level in DOK cells after treatment with arecoline (50 μM) or NNK (10 μM) for indicated times. GAPDH & alpha -tubulin were used as internal control. c ChIP assay of ADHFE1 & ALDH1A2 promoter region was performed with DOK cells using anti-DNMT3B antibody after treatment with vehicle control (DMSO, 10 nM), arecoline (50 μM) or NNK (10 μM) for 5 days. Mouse IgG (mIgG) antibody was used as negative control. d RT-PCR analysis of ADHFE1 & ALDH1A2 level in DOK cells after treatment with arecoline (50 μM) or NNK (10 μM) alone or combined with 5-aza-dC (5 μM) for 5 days. GAPDH was used as internal control. e qRT-PCR analysis of ADHFE1 & ALDH1A2 level in DOK cells after treatment with vehicle control (C), or arecoline (50 μM) plus control mimics (NC), miR-30a (20 nM), miR-379 (20 nM), or NNK (10 μM) plus control mimics (NC), miR-30a (20 nM), miR-379 (20 nM) for 5 days. GAPDH was used as an internal control. All data are presented as mean ± SD; **p < 0.01; ***p < 0.001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32238162), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-516_rabbit-polyclonal-dnmt3b-antibody-310202416212361.jpg)
![Western Blot: DNMT3B Antibody [NB300-516] - Arecoline & NNK induced DNMT3B activity & repressed ADHFE1, ALDH1A2 & miRNAs expression. a qRT–PCR analysis of miR-30a & miR-379 expression level after treatment with arecoline (50 μM) or NNK (10 μM) for indicated days. b RT-PCR analysis of ADHFE1, ALDH1A2 level & western blot analysis of DNMT3B level in DOK cells after treatment with arecoline (50 μM) or NNK (10 μM) for indicated times. GAPDH & alpha -tubulin were used as internal control. c ChIP assay of ADHFE1 & ALDH1A2 promoter region was performed with DOK cells using anti-DNMT3B antibody after treatment with vehicle control (DMSO, 10 nM), arecoline (50 μM) or NNK (10 μM) for 5 days. Mouse IgG (mIgG) antibody was used as negative control. d RT-PCR analysis of ADHFE1 & ALDH1A2 level in DOK cells after treatment with arecoline (50 μM) or NNK (10 μM) alone or combined with 5-aza-dC (5 μM) for 5 days. GAPDH was used as internal control. e qRT-PCR analysis of ADHFE1 & ALDH1A2 level in DOK cells after treatment with vehicle control (C), or arecoline (50 μM) plus control mimics (NC), miR-30a (20 nM), miR-379 (20 nM), or NNK (10 μM) plus control mimics (NC), miR-30a (20 nM), miR-379 (20 nM) for 5 days. GAPDH was used as an internal control. All data are presented as mean ± SD; **p < 0.01; ***p < 0.001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32238162), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-516_rabbit-polyclonal-dnmt3b-antibody-31020241621836.jpg)
