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![Western Blot: Peroxiredoxin 5 Antibody [NBP2-38370] - Analysis using Anti-PRDX5 antibody NBP2-38370 (A) shows similar pattern to independent antibody NBP2-38371 (B).](http://images.novusbio.com/fullsize2/Peroxiredoxin 5 Antibody-Western Blot-NBP2-38370-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Peroxiredoxin 5 Antibody [NBP2-38370] - Analysis using Anti-PRDX5 antibody NBP2-38370 (A) shows similar pattern to independent antibody NBP2-38371 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Peroxiredoxin 5 Antibody [NBP2-38370] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.](http://images.novusbio.com/fullsize2/Peroxiredoxin-5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38370-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Peroxiredoxin 5 Antibody [NBP2-38370] - Staining in human fallopian tube and liver tissues using anti-PRDX5 antibody. Corresponding PRDX5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/Peroxiredoxin-5-Antibody-Immunohistochemistry-Paraffin-NBP2-38370-img0008.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 5 Antibody [NBP2-38370] - Staining of human fallopian tube shows high expression.](http://images.novusbio.com/fullsize2/Peroxiredoxin-5-Antibody-Immunohistochemistry-Paraffin-NBP2-38370-img0006.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 5 Antibody [NBP2-38370] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize2/Peroxiredoxin-5-Antibody-Immunohistochemistry-Paraffin-NBP2-38370-img0007.jpg)
![Western Blot: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A: Hela Whole Cell Lysate Lane B: 293T Whole Cell LysateLane C: Jurkat Whole Cell Lysate Lane D: 293 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:22 kDaObserved band size:15 kDa](http://images.novusbio.com/fullsize2/Peroxiredoxin-5-Antibody-(002)-Western-Blot-NBP2-89862-img0002.jpg "Western Blot: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A: Hela Whole Cell Lysate Lane B: 293T Whole Cell LysateLane C: Jurkat Whole Cell Lysate Lane D: 293 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:22 kDaObserved band size:15 kDa")
![Immunocytochemistry/Immunofluorescence: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Staining of PRDX5 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human PRDX5 monoclonal antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize2/Peroxiredoxin-5-Antibody-(002)-Immunocytochemistry-Immunofluorescence-NBP2-89862-img0003.jpg)
![Immunoprecipitation: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg Jurkat Whole Cell LysateLane C:0.5 mg 293T Whole Cell Lysate 2 uL anti-PRDX5 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody:Anti-PRDX5 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 22 kDa Observed band size: 22 kDa](http://images.novusbio.com/fullsize2/Peroxiredoxin-5-Antibody-(002)-Immunoprecipitation-NBP2-89862-img0001.jpg)
![Knockout Validated: Peroxiredoxin 5 Antibody (002) [NBP2-89862] - Lane A: PRDX5 konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:22 kDa Observed band size:15 kDa](http://images.novusbio.com/fullsize2/Peroxiredoxin-5-Antibody-(002)-Knockout-Validated-NBP2-89862-img0004.jpg)
![Western Blot: Peroxiredoxin 5 Antibody [NBP2-38371] - Analysis using Anti-PRDX5 antibody NBP2-38371 (A) shows similar pattern to independent antibody NBP2-38370 (B).](http://images.novusbio.com/fullsize2/Peroxiredoxin-5-Antibody-Western-Blot-NBP2-38371-img0007.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Peroxiredoxin 5 Antibody [NBP2-38371] - Analysis using Anti-PRDX5 antibody NBP2-38371 (A) shows similar pattern to independent antibody NBP2-38370 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Peroxiredoxin 5 Antibody [NBP2-38371] - Staining in human fallopian tube and liver tissues using anti-PRDX5 antibody. Corresponding PRDX5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/Peroxiredoxin-5-Antibody-Immunohistochemistry-Paraffin-NBP2-38371-img0006.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 5 Antibody [NBP2-38371] - Staining of human fallopian tube shows high expression.](http://images.novusbio.com/fullsize2/Peroxiredoxin-5-Antibody-Immunohistochemistry-Paraffin-NBP2-38371-img0004.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 5 Antibody [NBP2-38371] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize2/Peroxiredoxin-5-Antibody-Immunohistochemistry-Paraffin-NBP2-38371-img0005.jpg)
![Western Blot: Peroxiredoxin 5 Antibody (6A10) [NBP2-59388] - Lane 1: Recombinant PRDX5 protein, Lane 2: MCF-7 cell lysates, Lane 3: A549 cell lysates, Lane 4: HeLa cell lysates, Lane 5: 293T cell lysates, Lane 6: Jurkat cell lysates](http://images.novusbio.com/fullsize2/Peroxiredoxin-5-Antibody-(6A10)-Western-Blot-NBP2-59388-img0001.jpg "Western Blot: Peroxiredoxin 5 Antibody (6A10) [NBP2-59388] - Lane 1: Recombinant PRDX5 protein, Lane 2: MCF-7 cell lysates, Lane 3: A549 cell lysates, Lane 4: HeLa cell lysates, Lane 5: 293T cell lysates, Lane 6: Jurkat cell lysates")
![Immunocytochemistry/Immunofluorescence: Peroxiredoxin 5 Antibody (6A10) [NBP2-59388] - Analysis of PRDX5 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PRDX5 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize2/Peroxiredoxin-5-Antibody-(6A10)-Immunocytochemistry-Immunofluorescence-NBP2-59388-img0002.jpg)
![Western Blot: Peroxiredoxin 5 Antibody [NBP1-58348] - Sample Tissue: Human Fetal Lung Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize2/Peroxiredoxin-5-Antibody-Western-Blot-NBP1-58348-img0004.jpg "Western Blot: Peroxiredoxin 5 Antibody [NBP1-58348] - Sample Tissue: Human Fetal Lung Antibody Dilution: 1.0 ug/ml")
![Western Blot: Peroxiredoxin 5 Antibody [NBP1-58348] - Titration: 0.2-1 ug/ml, Positive Control: Human Lung.](http://images.novusbio.com/fullsize2/Peroxiredoxin-5-Antibody-Western-Blot-NBP1-58348-img0003.jpg)
![Western Blot: Peroxiredoxin 5 Overexpression Lysate (Adult Normal) [NBL1-14742] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Peroxiredoxin 5.](http://images.novusbio.com/fullsize/Peroxiredoxin 5 Overexpression Lysate (Adult Normal)-Western Blot-NBL1-14742-img0002.jpg "Western Blot: Peroxiredoxin 5 Overexpression Lysate (Adult Normal) [NBL1-14742] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Peroxiredoxin 5.")
![Western Blot: Peroxiredoxin 5 Overexpression Lysate (Adult Normal) [NBP2-04918] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Peroxiredoxin 5.](http://images.novusbio.com/fullsize/Peroxiredoxin 5 Overexpression Lysate (Adult Normal)-Western Blot-NBP2-04918-img0001.jpg "Western Blot: Peroxiredoxin 5 Overexpression Lysate (Adult Normal) [NBP2-04918] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Peroxiredoxin 5.")
![ELISA: Human Peroxiredoxin 5 ELISA Kit (Colorimetric) [NBP2-60586] - Standard curve.](http://images.novusbio.com/fullsize2/Human-Peroxiredoxin-5-ELISA-Kit-(Colorimetric)-ELISA-NBP2-60586-img0001.jpg "ELISA: Human Peroxiredoxin 5 ELISA Kit (Colorimetric) [NBP2-60586] - Standard curve.")
![Sandwich ELISA: Peroxiredoxin 5 Antibody (3E6) [H00025824-M01] - Detection limit for recombinant GST tagged PRDX5 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize2/Peroxiredoxin 5 Antibody (3E6)-Sandwich ELISA-H00025824-M01-img0002.jpg "Sandwich ELISA: Peroxiredoxin 5 Antibody (3E6) [H00025824-M01] - Detection limit for recombinant GST tagged PRDX5 is approximately 0.3ng/ml as a capture antibody.")