| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79941 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55796-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14197 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-21067 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-21062 | |||
![SDS-Page: Recombinant Human Vinculin Protein [H00007414-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00007414-P01-1.jpg "SDS-Page: Recombinant Human Vinculin Protein [H00007414-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunohistochemistry-Paraffin: Vinculin Antibody (VCL/2573) - Azide and BSA Free [NBP2-79941] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Vinculin Mouse Monoclonal Antibody (VCL/2573).](http://images.novusbio.com/fullsize/Vinculin-Antibody-VCL-2573-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-79941-img0001.jpg "Immunohistochemistry-Paraffin: Vinculin Antibody (VCL/2573) - Azide and BSA Free [NBP2-79941] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Vinculin Mouse Monoclonal Antibody (VCL/2573).")
![Protein Array: Vinculin Antibody (VCL/2573) - Azide and BSA Free [NBP2-79941] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (mAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.](http://images.novusbio.com/fullsize/Vinculin-Antibody-VCL-2573-Azide-and-BSA-Free-Protein-Array-NBP2-79941-img0002.jpg)
![Immunohistochemistry-Paraffin: Vinculin Antibody (VCL/2573) [NBP2-79807] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Vinculin Antibody (VCL/2573).](http://images.novusbio.com/fullsize/Vinculin-Antibody-VCL-2573-Immunohistochemistry-Paraffin-NBP2-79807-img0001.jpg "Immunohistochemistry-Paraffin: Vinculin Antibody (VCL/2573) [NBP2-79807] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Vinculin Antibody (VCL/2573).")
![Protein Array: Vinculin Antibody (VCL/2573) [NBP2-79807] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Vinculin Antibody (VCL/2573) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5](http://images.novusbio.com/fullsize/Vinculin-Antibody-VCL-2573-Protein-Array-NBP2-79807-img0002.jpg)
![Western Blot: Vinculin Antibody [NBP2-55796] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Vinculin-Antibody-Western-Blot-NBP2-55796-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vinculin Antibody [NBP2-55796] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Vinculin Antibody [NBP2-55796] - Staining of human cell line U-2 OS shows localization to focal adhesion sites. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Vinculin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55796-img0001.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Vinculin Antibody [NBP2-55796] - Analysis in human cell line PC-3 and human cell line MCF-7.](http://images.novusbio.com/fullsize/Vinculin-Antibody-Western-Blot-NBP2-55796-img0002.jpg)
![Western Blot: Vinculin Antibody (9I3Y8) [NBP3-16145] - Western blot analysis of lysates from HeLa cells using Vinculin Rabbit mAb at 1:320000-1:1280000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-16145_rabbit-vinculin-mab-9i3y8-5220251265322.jpg "Western Blot: Vinculin Antibody (9I3Y8) [NBP3-16145] - Western blot analysis of lysates from HeLa cells using Vinculin Rabbit mAb at 1:320000-1:1280000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")
![Immunocytochemistry/ Immunofluorescence: Vinculin Antibody (9I3Y8) [NBP3-16145] - Confocal imaging of paraffin-embedded Human colon cancer tissue using Vinculin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-16145_rabbit-vinculin-mab-9i3y8-522025125423.jpg)
![Western Blot: Vinculin Antibody (3F8-1D4) [H00007414-M02] - Vinculin monoclonal antibody, clone 3F8-1D4. Western Blot analysis of VCL expression in HeLa.](http://images.novusbio.com/fullsize/Vinculin-Antibody-3F8-1D4-Western-Blot-H00007414-M02-img0005.jpg "Western Blot: Vinculin Antibody (3F8-1D4) [H00007414-M02] - Vinculin monoclonal antibody, clone 3F8-1D4. Western Blot analysis of VCL expression in HeLa.")
![Immunocytochemistry/Immunofluorescence: Vinculin Antibody (3F8-1D4) [H00007414-M02] - Analysis of monoclonal antibody to VCL on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Vinculin-Antibody-3F8-1D4-Immunocytochemistry-Immunofluorescence-H00007414-M02-img0003.jpg)
![ELISA: Vinculin Antibody (3F8-1D4) [H00007414-M02] - Detection limit for recombinant GST tagged VCL is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Vinculin-Antibody-3F8-1D4-ELISA-H00007414-M02-img0004.jpg)
![Immunohistochemistry-Paraffin: Vinculin Antibody (VCL/7091R) [NBP3-13989] - Formalin-fixed, paraffin-embedded human placentastained with Vinculin antibody (VCL/7091R) at 2ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.](http://images.novusbio.com/fullsize/Vinculin-Antibody-VCL-7091R-Immunohistochemistry-Paraffin-NBP3-13989-img0001.jpg "Immunohistochemistry-Paraffin: Vinculin Antibody (VCL/7091R) [NBP3-13989] - Formalin-fixed, paraffin-embedded human placentastained with Vinculin antibody (VCL/7091R) at 2ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.")
![Immunohistochemistry-Paraffin: Vinculin Antibody (VCL/7091R) [NBP3-13989] - Formalin-fixed, paraffin-embedded human pancreasstained with Vinculin antibody (VCL/7091R) at 2ug/ml.](http://images.novusbio.com/fullsize/Vinculin-Antibody-VCL-7091R-Immunohistochemistry-Paraffin-NBP3-13989-img0002.jpg)
![Western Blot: Vinculin Overexpression Lysate (Adult Normal) [NBL1-17706] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Vinculin.](http://images.novusbio.com/fullsize/Vinculin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17706-img0002.jpg "Western Blot: Vinculin Overexpression Lysate (Adult Normal) [NBL1-17706] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Vinculin.")
