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![Western Blot: UBE1 Overexpression Lysate (Adult Normal) [NBL1-17494] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UBE1.](http://images.novusbio.com/fullsize/UBE1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17494-img0002.jpg "Western Blot: UBE1 Overexpression Lysate (Adult Normal) [NBL1-17494] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for UBE1.")
![Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody (05) [NBP3-05789] - Lane A: HepG2 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:111 kDaObserved band size:100 kDa](http://images.novusbio.com/fullsize/Ubiquitin-activating-Enzyme-UBE1-Antibody-05-Western-Blot-NBP3-05789-img0001.jpg "Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody (05) [NBP3-05789] - Lane A: HepG2 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:111 kDaObserved band size:100 kDa")
![Immunoprecipitation: Ubiquitin-activating Enzyme/UBE1 Antibody (05) [NBP3-05789] - Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg K562 Whole Cell Lysate0.5 uL anti-UBA1 mouse monoclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-UBA1 mouse monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 111 kDaObserved band size: 111 kDa](http://images.novusbio.com/fullsize/Ubiquitin-activating-Enzyme-UBE1-Antibody-05-Immunoprecipitation-NBP3-05789-img0002.jpg)