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![Western Blot: Septin-12 Antibody [H00124404-B01P] - Analysis of SEPT12 expression in transfected 293T cell line by SEPT12 polyclonal antibody. Lane 1: SEPT12 transfected lysate(39.38 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Septin-12-Antibody-Western-Blot-H00124404-B01P-img0006.jpg "Western Blot: Septin-12 Antibody [H00124404-B01P] - Analysis of SEPT12 expression in transfected 293T cell line by SEPT12 polyclonal antibody. Lane 1: SEPT12 transfected lysate(39.38 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Septin-12 Antibody [H00124404-B01P] - An IFA revealed multiple localizations of SEPT12 and LAMINB1 signals. A total of 32 male germ cells were were evaluated as (a.) Round spermatids (n = 9; labeled with anti-SEPT12 and anti-LAMINB1 antibodies as the presented figure,9/9); (b.) elongating and (c.) elongated spermatids (n = 11;11/11); (d.) ejaculated spermatozoa (n = 10;10/10). (a.-d.) Anti-SEPT12 antibody (left panel; green), anti-LAMINB1 antibody (middle panel; red), and a combination of the left and middle panels (right panel). The arrow indicates SEPT12 or LAMINB1 signals. Scale bar: 5 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0120722) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Septin-12-Antibody-Immunocytochemistry-Immunofluorescence-H00124404-B01P-img0007.jpg)
![Immunohistochemistry-Paraffin: Septin-12 Antibody [H00124404-B01P] - Analysis of purified antibody to SEPT12 on formalin-fixed paraffin-embedded human colon. (antibody concentration 3 ug/ml)](http://images.novusbio.com/fullsize/Septin-12-Antibody-Immunohistochemistry-Paraffin-H00124404-B01P-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Septin-12 Antibody [H00124404-B01P] - Analysis of purified antibody to SEPT12 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/Septin-12-Antibody-Immunocytochemistry-Immunofluorescence-H00124404-B01P-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Septin-12 Antibody [H00124404-B01P] - SEPT12 interacts & colocalizes with LAMINB1 male germ cells.(A) Co-IP of FLAG-SPAG4 & LAIMINB1 with SEPT12-GFP. The cell lysates of the NT2D1 cells, transfected with pEGFP-SEPT12 & a pFLAG-SPAG4 vector, are subjected to IP with an anti-GFP antibody (right panel, Row 3) or a nonspecific control IgG (right panel, Row 2), followed by IB with an anti-GFP, anti-FLAG, or anti-LAMINB1 antibody. An input protein (5%) was used as a control during the IB of the transfected cell lysates (right panel, Row 1). (B) An IFA revealed multiple localizations of SEPT12 & LAMINB1 signals. A total of 32 male germ cells were were evaluated as (A) Round spermatids (n = 9; labeled with anti-SEPT12 & anti-LAMINB1antibodies as the presented figure,9/9); (B) elongating & (C) elongated spermatids (n = 11;11/11); (D) ejaculated spermatozoa (n = 10;10/10). (A–D) Anti-SEPT12 antibody (left panel; green), anti-LAMINB1 antibody (middle panel; red), & a combination of the left & middle panels (right panel). The arrow indicates SEPT12 or LAMINB1 signals. Scale bar: 5 μm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0120722), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00124404-b01p_mouse-polyclonal-septin-12-antibody-310202415483631.jpg)
![Western Blot: Septin-12 Antibody [NBP2-20300] - Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Septin-12-Antibody-Western-Blot-NBP2-20300-img0001.jpg "Western Blot: Septin-12 Antibody [NBP2-20300] - Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Septin-12 Antibody [NBP2-20300] - Sample: A431 cells were fixed in iced-cold MeOH for 5 min. Green: SEPT12 protein stained by SEPT12 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Septin-12-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20300-img0003.jpg)
![Immunohistochemistry-Paraffin: Septin-12 Antibody [NBP2-20300] - Immunohistochemical analysis of paraffin-embedded hela xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Septin-12-Antibody-Immunohistochemistry-Paraffin-NBP2-20300-img0002.jpg)
![Western Blot: Septin-12 Antibody [NBP1-91640] - Analysis in control (vector only transfected HEK293T lysate) and LY408232 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Septin-12-Antibody-Western-Blot-NBP1-91640-img0004.jpg "Western Blot: Septin-12 Antibody [NBP1-91640] - Analysis in control (vector only transfected HEK293T lysate) and LY408232 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: Septin-12 Antibody [NBP1-91640] - Staining of human cell line U-2 OS shows localization to microtubules. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Septin-12-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91640-img0005.jpg)
![Western Blot: Septin-12 Antibody [NBP1-58117] - 721_B cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/Septin-12-Antibody-Western-Blot-NBP1-58117-img0002.jpg "Western Blot: Septin-12 Antibody [NBP1-58117] - 721_B cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: Septin-12, variant 2 Overexpression Lysate (Adult Normal) [NBL1-07153] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Septin-12, variant 2.](http://images.novusbio.com/fullsize/Septin-12-variant-2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07153-img0002.jpg "Western Blot: Septin-12, variant 2 Overexpression Lysate (Adult Normal) [NBL1-07153] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Septin-12, variant 2.")