| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84045-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22627-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38920-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-15573-20ul | |||
![Immunocytochemistry/Immunofluorescence: SEC62 Antibody [NBP1-84045] - Staining of human cell line A-431 shows positivity in endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SEC62-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84045-img0004.jpg "Immunocytochemistry/Immunofluorescence: SEC62 Antibody [NBP1-84045] - Staining of human cell line A-431 shows positivity in endoplasmic reticulum. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: SEC62 Antibody [NBP1-84045] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/SEC62-Antibody-Immunohistochemistry-Paraffin-NBP1-84045-img0011.jpg)
![Immunohistochemistry-Paraffin: SEC62 Antibody [NBP1-84045] - Staining of human skeletal muscle shows weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/SEC62-Antibody-Immunohistochemistry-Paraffin-NBP1-84045-img0008.jpg)
![Immunohistochemistry-Paraffin: SEC62 Antibody [NBP1-84045] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/SEC62-Antibody-Immunohistochemistry-Paraffin-NBP1-84045-img0009.jpg)
![Immunohistochemistry-Paraffin: SEC62 Antibody [NBP1-84045] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/SEC62-Antibody-Immunohistochemistry-Paraffin-NBP1-84045-img0010.jpg)
![Western Blot: SEC62 Antibody [NBP2-81813] - Analysis of SEC62 in rat brain tissue lysate with SEC62 antibody at (A) 0.5 and (B) 1 ug/ml.](http://images.novusbio.com/fullsize/SEC62-Antibody-Western-Blot-NBP2-81813-img0001.jpg "Western Blot: SEC62 Antibody [NBP2-81813] - Analysis of SEC62 in rat brain tissue lysate with SEC62 antibody at (A) 0.5 and (B) 1 ug/ml.")
![Immunohistochemistry: SEC62 Antibody [NBP2-81813] - Immunohistochemistry of SEC62 in mouse brain tissue with SEC62 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/SEC62-Antibody-Immunohistochemistry-NBP2-81813-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: SEC62 Antibody - BSA Free [NBP2-81813] - Immunofluorescence of SEC62 in mouse brain tissue with SEC62 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-81813_rabbit-polyclonal-sec62-antibody-271120242074636.jpg)
![Western Blot: SEC62 Antibody (JE62-80) [NBP3-32941] - Western blot analysis of SEC62 on different lysates with Rabbit anti-SEC62 antibody (NBP3-32941) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: HepG2 cell lysate Lane 4: K-562 cell lysate Lane 5: A431 cell lysate Lane 6: HEK-293 cell lysate Lane 7: PANC-1 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 46 kDa Observed band size: 50 kDa Exposure time: 4 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32941) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32941_rabbit-sec62-mab-je62-80-28820241244293.jpg "Western Blot: SEC62 Antibody (JE62-80) [NBP3-32941] - Western blot analysis of SEC62 on different lysates with Rabbit anti-SEC62 antibody (NBP3-32941) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: HepG2 cell lysate Lane 4: K-562 cell lysate Lane 5: A431 cell lysate Lane 6: HEK-293 cell lysate Lane 7: PANC-1 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 46 kDa Observed band size: 50 kDa Exposure time: 4 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32941) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SEC62 Antibody (JE62-80) [NBP3-32941] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SEC62 antibody (NBP3-32941) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32941) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32941_rabbit-sec62-mab-je62-80-298202414161819.jpg)
![Flow Cytometry: SEC62 Antibody (JE62-80) [NBP3-32941] - Flow cytometric analysis of HeLa cells labeling SEC62. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32941, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32941_rabbit-sec62-mab-je62-80-29202416453268.jpg)
![Western Blot: SEC62 Antibody (SR1865) [NBP3-22627] - Western blot analysis using NBP3-22627 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22627_rabbit-sec62-mab-sr1865-57202314565419.jpg "Western Blot: SEC62 Antibody (SR1865) [NBP3-22627] - Western blot analysis using NBP3-22627 in HeLa cell lysate.")
![Immunoprecipitation:SEC62 AntibodyNBP3-29517] -Detection of human Sec62 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Sec62 antibody NBP3-29517 (lot NBP3-29517-1) used for IP at 6 µg/mg lysate. Sec62 was also immunoprecipitated by rabbit anti-Sec62 antibody . For blotting immunoprecipitated Sec62, NBP3-29517 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29517_rabbit-sec62-pab-3012202418231.jpg "Immunoprecipitation:SEC62 AntibodyNBP3-29517] -Detection of human Sec62 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Sec62 antibody NBP3-29517 (lot NBP3-29517-1) used for IP at 6 µg/mg lysate. Sec62 was also immunoprecipitated by rabbit anti-Sec62 antibody . For blotting immunoprecipitated Sec62, NBP3-29517 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: SEC62 Antibody (PSH0-25) [NBP3-32939] - Western blot analysis of SEC62 on different lysates with Rabbit anti-SEC62 antibody (NBP3-32939) at 1/5,000 dilution. Lane 1: Hela cell lysate Lane 2: Hela cell lysate Lane 3: K562 cell lysate Lane 4: SH-SY5Y cell lysate Lane 5: HepG2 cell lysate Lane 6: 293T cell lysate Lane 7: Jurkat cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 46 kDa Observed band size: 56 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32939) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32939_rabbit-sec62-mab-psh0-25-288202412461683.jpg "Western Blot: SEC62 Antibody (PSH0-25) [NBP3-32939] - Western blot analysis of SEC62 on different lysates with Rabbit anti-SEC62 antibody (NBP3-32939) at 1/5,000 dilution. Lane 1: Hela cell lysate Lane 2: Hela cell lysate Lane 3: K562 cell lysate Lane 4: SH-SY5Y cell lysate Lane 5: HepG2 cell lysate Lane 6: 293T cell lysate Lane 7: Jurkat cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 46 kDa Observed band size: 56 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32939) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Western Blot: SEC62 Antibody (PSH0-91) [NBP3-32940] - Western blot analysis of SEC62 on different lysates with Rabbit anti-SEC62 antibody (NBP3-32940) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: SH-SY5Y cell lysate (20 ug/Lane) Lane 4: HepG2 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: MCF7 cell lysate (20 ug/Lane) Lane 8: Daudi cell lysate (20 ug/Lane) Lane 9: PC-12 cell lysate (20 ug/Lane) Lane 10: Mouse liver tissue lysate (40 ug/Lane) Lane 11: Mouse brain tissue lysate (40 ug/Lane) Lane 12: Rat spleen tissue lysate (40 ug/Lane) Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32940) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32940_rabbit-sec62-mab-psh0-91-288202412461647.jpg "Western Blot: SEC62 Antibody (PSH0-91) [NBP3-32940] - Western blot analysis of SEC62 on different lysates with Rabbit anti-SEC62 antibody (NBP3-32940) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: SH-SY5Y cell lysate (20 ug/Lane) Lane 4: HepG2 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: MCF7 cell lysate (20 ug/Lane) Lane 8: Daudi cell lysate (20 ug/Lane) Lane 9: PC-12 cell lysate (20 ug/Lane) Lane 10: Mouse liver tissue lysate (40 ug/Lane) Lane 11: Mouse brain tissue lysate (40 ug/Lane) Lane 12: Rat spleen tissue lysate (40 ug/Lane) Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32940) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SEC62 Antibody [NBP2-38920] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells, glial cells and endothelial cells.](http://images.novusbio.com/fullsize/SEC62-Antibody-Immunohistochemistry-NBP2-38920-img0001.jpg "Immunohistochemistry: SEC62 Antibody [NBP2-38920] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells, glial cells and endothelial cells.")
![Western Blot: SEC62 Antibody [NBP1-98450] - NCI-H226 Cell Lysate 1.0ug/ml, Gel Concentration: 12%](http://images.novusbio.com/fullsize/SEC62-Antibody-Western-Blot-NBP1-98450-img0002.jpg "Western Blot: SEC62 Antibody [NBP1-98450] - NCI-H226 Cell Lysate 1.0ug/ml, Gel Concentration: 12%")
![Western Blot: SEC62 Overexpression Lysate (Adult Normal) [NBP2-08026] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SEC62.](http://images.novusbio.com/fullsize/SEC62-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08026-img0001.jpg "Western Blot: SEC62 Overexpression Lysate (Adult Normal) [NBP2-08026] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SEC62.")
![Western Blot: SEC62 Antibody - Azide and BSA Free [SEC62] - Western blot analysis of various lysates using SEC62 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-15573_rabbit-sec62-pab-52202517344297.jpg "Western Blot: SEC62 Antibody - Azide and BSA Free [SEC62] - Western blot analysis of various lysates using SEC62 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 45s.")