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![Immunohistochemistry-Paraffin: SDF4 Antibody [NBP2-47284] - Staining in human small intestine and pancreas tissues using anti-SDF4 antibody. Corresponding SDF4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SDF4-Antibody-Immunohistochemistry-Paraffin-NBP2-47284-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SDF4 Antibody [NBP2-47284] - Staining in human small intestine and pancreas tissues using anti-SDF4 antibody. Corresponding SDF4 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SDF4 Antibody [NBP2-47284] - Staining of human cell line U-251 MG shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SDF4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47284-img0007.jpg)
![Immunohistochemistry-Paraffin: SDF4 Antibody [NBP2-47284] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/SDF4-Antibody-Immunohistochemistry-Paraffin-NBP2-47284-img0004.jpg)
![Immunohistochemistry-Paraffin: SDF4 Antibody [NBP2-47284] - Staining of human small intestine shows high expression.](http://images.novusbio.com/fullsize/SDF4-Antibody-Immunohistochemistry-Paraffin-NBP2-47284-img0005.jpg)
![Immunohistochemistry: SDF4 Antibody [NBP3-35551] - Immunohistochemistry analysis of paraffin-embedded Human colon using SDF4 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35551_rabbit-sdf4-pab-1012202411381217.jpg "Immunohistochemistry: SDF4 Antibody [NBP3-35551] - Immunohistochemistry analysis of paraffin-embedded Human colon using SDF4 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Western Blot: SDF4 Antibody [NBP3-35551] - Western blot analysis of various lysates using SDF4 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35551_rabbit-sdf4-pab-1012202411343312.jpg)
![Western Blot: SDF4 Antibody [NBP3-35551] - Western blot analysis of various lysates using SDF4 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-35551_rabbit-sdf4-pab-101220241136025.jpg)
![Western Blot: SDF4 Antibody [NBP2-20276] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/SDF4-Antibody-Western-Blot-NBP2-20276-img0001.jpg "Western Blot: SDF4 Antibody [NBP2-20276] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: SDF4 Antibody [NBP2-20276] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/SDF4-Antibody-Immunohistochemistry-Paraffin-NBP2-20276-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: SDF4 Antibody [NBP2-98650] - Immunofluorescence staining of SDF4 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SDF4 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/SDF4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98650-img0001.jpg "Immunocytochemistry/Immunofluorescence: SDF4 Antibody [NBP2-98650] - Immunofluorescence staining of SDF4 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SDF4 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.")
![Immunohistochemistry-Paraffin: SDF4 Antibody [NBP2-98650] - Immunochemical staining of human SDF4 in human small intestine with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/SDF4-Antibody-Immunohistochemistry-Paraffin-NBP2-98650-img0003.jpg)
![Immunohistochemistry-Paraffin: SDF4 Antibody [NBP2-98650] - Immunochemical staining of human SDF4 in human gallbladder with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/SDF4-Antibody-Immunohistochemistry-Paraffin-NBP2-98650-img0002.jpg)
![SDS-Page: Recombinant Human SDF4 Protein [H00051150-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-SDF4-Protein-SDS-Page-H00051150-P01-img0001.jpg "SDS-Page: Recombinant Human SDF4 Protein [H00051150-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![ELISA: Human SDF4 ELISA Kit (Colorimetric) [NBP2-75386] - Samples were spiked with high concentrations of Human SDF4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75386_human-sdf4-elisa-kit-colorimetric-13220241620492.jpg "ELISA: Human SDF4 ELISA Kit (Colorimetric) [NBP2-75386] - Samples were spiked with high concentrations of Human SDF4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human SDF4 ELISA Kit (Colorimetric) [NBP2-75386] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75386_human-sdf4-elisa-kit-colorimetric-432024856747.jpg)
![Western Blot: SDF4 Antibody [NBP1-69311] - This Anti-SDF4 antibody was used in Western Blot of Jurkat tissue lysate at a concentration of 2.5ug/ml.](http://images.novusbio.com/fullsize/SDF4-Antibody-Western-Blot-NBP1-69311-img0001.jpg "Western Blot: SDF4 Antibody [NBP1-69311] - This Anti-SDF4 antibody was used in Western Blot of Jurkat tissue lysate at a concentration of 2.5ug/ml.")
![Western Blot: SDF4 Antibody [NBP1-62577] - Human Small Intestine, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/SDF4-Antibody-Western-Blot-NBP1-62577-img0002.jpg "Western Blot: SDF4 Antibody [NBP1-62577] - Human Small Intestine, concentration 0.2-1 ug/ml.")
![ELISA: Mouse SDF4 ELISA Kit (Colorimetric) [NBP2-75384] - Samples were spiked with high concentrations of Mouse SDF4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75384_mouse-sdf4-elisa-kit-colorimetric-13220241692247.jpg "ELISA: Mouse SDF4 ELISA Kit (Colorimetric) [NBP2-75384] - Samples were spiked with high concentrations of Mouse SDF4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse SDF4 ELISA Kit (Colorimetric) [NBP2-75384] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75384_mouse-sdf4-elisa-kit-colorimetric-4320249163339.jpg)
![ELISA: Rat SDF4 ELISA Kit (Colorimetric) [NBP2-75385] - Samples were spiked with high concentrations of Rat SDF4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75385_rat-sdf4-elisa-kit-colorimetric-132202416133656.jpg "ELISA: Rat SDF4 ELISA Kit (Colorimetric) [NBP2-75385] - Samples were spiked with high concentrations of Rat SDF4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat SDF4 ELISA Kit (Colorimetric) [NBP2-75385] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75385_rat-sdf4-elisa-kit-colorimetric-432024955033.jpg)