HAF109). A specific band was detected for c-Rel at approximately 69 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of A20 mouse B cell lymphoma cell line, and CH-1 mouse B cell lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse c-Rel Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2699) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for c-Rel at approximately 69 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2699-SP | |||
HAF005). A specific band was detected for c-Rel at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of DA3 mouse myeloma cell line, A20 mouse B cell lymphoma cell line, and CH-1 mouse B cell lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse c-Rel Monoclonal Antibody (Catalog # MAB2699) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for c-Rel at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2699-SP | |||
HAF005). A specific band was detected for c-Rel at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rat Anti-Human c-Rel Monoclonal Antibody (Catalog # MAB4606) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for c-Rel at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4606-SP | |||
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />
![Western Blot: c-Rel Antibody (1E7) [NBP2-37593] - Analysis using c-Rel mouse mAb against Jurkat (1), NIH/3T3 (2), Hela (3), HEK293 (4) and RAJI (5) cell lysate.](http://images.novusbio.com/fullsize/c-Rel-Antibody-1E7-Western-Blot-NBP2-37593-img0002.jpg "Western Blot: c-Rel Antibody (1E7) [NBP2-37593] - Analysis using c-Rel mouse mAb against Jurkat (1), NIH/3T3 (2), Hela (3), HEK293 (4) and RAJI (5) cell lysate.")
![Immunocytochemistry/Immunofluorescence: c-Rel Antibody (1E7) [NBP2-37593] - Analysis of U251 cells using c-Rel mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/c-Rel-Antibody-1E7-Immunocytochemistry-Immunofluorescence-NBP2-37593-img0003.jpg)
![Immunohistochemistry-Paraffin: c-Rel Antibody (1E7) [NBP2-37593] - Analysis of endometrial cancer tissues (left) and liver cancer tissues (right) using c-Rel mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/c-Rel-Antibody-1E7-Immunohistochemistry-NBP2-37593-img0004.jpg)
![ELISA: c-Rel Antibody (1E7) [NBP2-37593] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/c-Rel-Antibody-1E7-ELISA-NBP2-37593-img0001.jpg)
NL013) and counterstained with nuclear stain (green). View our protocol for ![Western Blot: c-Rel Antibody [NB600-474] - All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development.](http://images.novusbio.com/fullsize/c-Rel-Antibody-Western-Blot-NB600-474-img0005.jpg "Western Blot: c-Rel Antibody [NB600-474] - All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development.")
![Western blot of HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development.](http://images.novusbio.com/fullsize/nb600-474_rabbit-polyclonal-c-rel-antibody-255202313292313.jpg)
![Western Blot: c-Rel Antibody [NBP1-52021] - Analysis of c-Rel in Human Spleen lysate (35ug protein in RIPA buffer) using this antibody at 0.5 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/c-Rel-Antibody-Western-Blot-NBP1-52021-img0002.jpg "Western Blot: c-Rel Antibody [NBP1-52021] - Analysis of c-Rel in Human Spleen lysate (35ug protein in RIPA buffer) using this antibody at 0.5 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: c-Rel Antibody [NB100-97834] - Detection of Human c-Rel by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-c-Rel antibody NB100-97834 used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. c-Rel was also immunoprecipitated by rabbit anti-c-Rel antibody NB100-97833, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).](http://images.novusbio.com/fullsize/c-Rel-Antibody-Western-Blot-NB100-97834-img0001.jpg "Western Blot: c-Rel Antibody [NB100-97834] - Detection of Human c-Rel by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-c-Rel antibody NB100-97834 used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. c-Rel was also immunoprecipitated by rabbit anti-c-Rel antibody NB100-97833, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).")
