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![Western Blot: PAK1 Antibody [NBP2-95107] - Western blot analysis of extracts of various cell lines, using PAK1 antibody (NBP2-95107) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/PAK1 Antibody-Western Blot-NBP2-95107-img0001.jpg "Western Blot: PAK1 Antibody [NBP2-95107] - Western blot analysis of extracts of various cell lines, using PAK1 antibody (NBP2-95107) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western Blot: PAK1 Antibody - Azide and BSA Free [PAK1] - Western blot analysis of various lysates using PAK1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp2-95107_rabbit-polyclonal-pak1-antibody-5220251638053.jpg)
![Western Blot: PAK1 [p Thr84] Antibody [NBP2-29511] - Western blot of mouse hippocampal lysate showing specific immunolabeling of the ~68k PAK1 protein phosphorylated at Thr84 (control). Phosphospecificity is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (800 units/1mg protein for 30min). The immunolabeling is completely eliminated by treatment with lambda-Ptase.](http://images.novusbio.com/fullsize/PAK1-[p-Thr84]-Antibody-Western-Blot-NBP2-29511-img0002.jpg "Western Blot: PAK1 [p Thr84] Antibody [NBP2-29511] - Western blot of mouse hippocampal lysate showing specific immunolabeling of the ~68k PAK1 protein phosphorylated at Thr84 (control). Phosphospecificity is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (800 units/1mg protein for 30min). The immunolabeling is completely eliminated by treatment with lambda-Ptase.")
![Western Blot: PAK1/2/3 [p Ser141, p Ser139, p Ser144] Antibody (1T7A5) [NBP3-16930] - Western blot analysis of extracts of various cell lines, using PAK1/2/3 Rabbit mAb (NBP3-16930) at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (30 uM) at 37C for 30 minutes after serum-starvation overnight Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PAK1-2-3-p-Ser141-p-Ser139-p-Ser144-Antibody-1T7A5-Western-Blot-NBP3-16930-img0001.jpg "Western Blot: PAK1/2/3 [p Ser141, p Ser139, p Ser144] Antibody (1T7A5) [NBP3-16930] - Western blot analysis of extracts of various cell lines, using PAK1/2/3 Rabbit mAb (NBP3-16930) at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (30 uM) at 37C for 30 minutes after serum-starvation overnight Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: PAK1/2/3 [p Thr402] Antibody [NB100-723] - Rat hippocampal lysate showing specific immunolabeling of the ~68k to ~70k PAK protein (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in -Ptase (1200 units for 30 min) before being exposed to the phospho-Thr402 PAK-1,2,3 antibody. The immunolabeling of PAK is completely eliminated by treatment with lambda-phosphatase.](http://images.novusbio.com/fullsize/PAK1-2-3-[p-Thr402]-Antibody-Western-Blot-NB100-723-img0001.jpg "Western Blot: PAK1/2/3 [p Thr402] Antibody [NB100-723] - Rat hippocampal lysate showing specific immunolabeling of the ~68k to ~70k PAK protein (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in -Ptase (1200 units for 30 min) before being exposed to the phospho-Thr402 PAK-1,2,3 antibody. The immunolabeling of PAK is completely eliminated by treatment with lambda-phosphatase.")
![Western Blot: PAK1 Overexpression Lysate (Adult Normal) [NBP2-09482] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PAK1.](http://images.novusbio.com/fullsize/PAK1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09482-img0001.jpg "Western Blot: PAK1 Overexpression Lysate (Adult Normal) [NBP2-09482] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PAK1.")
![Western Blot: PAK1 [p Ser204] Antibody [NBP2-29665] - Untreated and pervanadate-treated NIH/3T3 cells (lanes 1 and 2, respectively) were resolved by electrophoresis, transferred to PVDF and probed with anti-phospho-PAK1 (Ser204) (1:1,000). Blocking Peptide Analysis: Pervanadate-treated NIH/3T3 lysate was either unblocked, phosphorylated-peptide blocked, or non-phosphorylated peptide blocked (lanes 3, 4, and 5, respectively) that are that were resolved by electrophoresis, transferred to PVDF and probed with anti-phospho-PAK1 (Ser204) (1:1,000). Lanes 4 and 5 were blocking peptides were the phosphorylated peptide used as the immunogen and the non-phopshrylated form of the peptide. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phosphorylated PAK1.](http://images.novusbio.com/fullsize/PAK1-[p-Ser204]-Antibody-Western-Blot-NBP2-29665-img0001.jpg "Western Blot: PAK1 [p Ser204] Antibody [NBP2-29665] - Untreated and pervanadate-treated NIH/3T3 cells (lanes 1 and 2, respectively) were resolved by electrophoresis, transferred to PVDF and probed with anti-phospho-PAK1 (Ser204) (1:1,000). Blocking Peptide Analysis: Pervanadate-treated NIH/3T3 lysate was either unblocked, phosphorylated-peptide blocked, or non-phosphorylated peptide blocked (lanes 3, 4, and 5, respectively) that are that were resolved by electrophoresis, transferred to PVDF and probed with anti-phospho-PAK1 (Ser204) (1:1,000). Lanes 4 and 5 were blocking peptides were the phosphorylated peptide used as the immunogen and the non-phopshrylated form of the peptide. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phosphorylated PAK1.")