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![Western Blot: iNOS Antibody (M398) [NBP3-23204] - Western blot analysis of mouse macrophages untreated (lanes 1 & 3) or treated with LPS (1ug/ml) for 18 hrs (lanes 2 & 4). The blots were probed with mouse monoclonal anti-iNOS at 1:500 (NBP3-23204) (lanes 1 & 2) or rabbit polyclonal anti-iNOS at 1:250 (NBP3-23216) (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23204_mouse-inos-mab-m398-19920231410117.jpg "Western Blot: iNOS Antibody (M398) [NBP3-23204] - Western blot analysis of mouse macrophages untreated (lanes 1 & 3) or treated with LPS (1ug/ml) for 18 hrs (lanes 2 & 4). The blots were probed with mouse monoclonal anti-iNOS at 1:500 (NBP3-23204) (lanes 1 & 2) or rabbit polyclonal anti-iNOS at 1:250 (NBP3-23216) (lanes 3 & 4).")
![Western Blot: iNOS Antibody [NBP3-23216] - Western blot analysis of mouse macrophages untreated (lanes 1 & 3) or treated with LPS (1ug/ml) for 18 hrs (lanes 2 & 4). The blots were probed with mouse monoclonal anti-iNOS at 1:500 (NBP3-23204) (lanes 1 & 2) or rabbit polyclonal anti-iNOS at 1:250 (NBP3-23216) (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23216_rabbit-inos-pab-199202312523648.jpg "Western Blot: iNOS Antibody [NBP3-23216] - Western blot analysis of mouse macrophages untreated (lanes 1 & 3) or treated with LPS (1ug/ml) for 18 hrs (lanes 2 & 4). The blots were probed with mouse monoclonal anti-iNOS at 1:500 (NBP3-23204) (lanes 1 & 2) or rabbit polyclonal anti-iNOS at 1:250 (NBP3-23216) (lanes 3 & 4).")
![Western Blot: iNOS Overexpression Lysate (Adult Normal) [NBL1-13721] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for iNOS.](http://images.novusbio.com/fullsize/iNOS-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13721-img0002.jpg "Western Blot: iNOS Overexpression Lysate (Adult Normal) [NBL1-13721] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for iNOS.")
![Western Blot: iNOS Antibody (JE59-61) [NBP3-32472] - Western blot analysis of iNOS on different lysates with Rabbit anti-iNOS antibody (NBP3-32472) at 1/1,000 dilution. Lane 1: RAW264.7 whole cell lysate Lane 2: RAW264.7 treated with 1ug/mL LPS for 24 hours whole cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 131 kDa Observed band size: 131 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32472) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32472_rabbit-inos-mab-je59-61-27820241631695.jpg "Western Blot: iNOS Antibody (JE59-61) [NBP3-32472] - Western blot analysis of iNOS on different lysates with Rabbit anti-iNOS antibody (NBP3-32472) at 1/1,000 dilution. Lane 1: RAW264.7 whole cell lysate Lane 2: RAW264.7 treated with 1ug/mL LPS for 24 hours whole cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 131 kDa Observed band size: 131 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32472) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Western Blot: iNOS Antibody [NBP2-42880] - Untreated (--) and treated (+) Raw264.7 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with iNOS antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/iNOS-Antibody-Western-Blot-NBP2-42880-img0004.jpg "Western Blot: iNOS Antibody [NBP2-42880] - Untreated (--) and treated (+) Raw264.7 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with iNOS antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![ELISA: Human iNOS ELISA Kit (Colorimetric) [NBP2-80255] - Samples were spiked with high concentrations of Human iNOS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-80255_human-inos-elisa-kit-colorimetric-132202416201937.jpg "ELISA: Human iNOS ELISA Kit (Colorimetric) [NBP2-80255] - Samples were spiked with high concentrations of Human iNOS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human iNOS ELISA Kit (Colorimetric) [NBP2-80255] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-80255_human-inos-elisa-kit-colorimetric-43202485971.jpg)
![ELISA: Mouse iNOS ELISA Kit (Colorimetric) [NBP2-80256] - Samples were spiked with high concentrations of Mouse iNOS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-80256_mouse-inos-elisa-kit-colorimetric-13220241622103.jpg "ELISA: Mouse iNOS ELISA Kit (Colorimetric) [NBP2-80256] - Samples were spiked with high concentrations of Mouse iNOS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse iNOS ELISA Kit (Colorimetric) [NBP2-80256] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-80256_mouse-inos-elisa-kit-colorimetric-432024903527.jpg)
![Immunocytochemistry/Immunofluorescence: iNOS Antibody [NBP2-99091] - Immunofluorescence staining of iNOS in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human iNOS polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/iNOS-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99091-img0001.jpg "Immunocytochemistry/Immunofluorescence: iNOS Antibody [NBP2-99091] - Immunofluorescence staining of iNOS in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human iNOS polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm.")