| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24727SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-15592-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33985-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22101-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81262-25ul | |||
![N/A CXCL8/IL-8 [Biotin]](http://images.novusbio.com/fullsize2/DATA_IL8_DY208_ELISA_2307.jpg "N/A CXCL8/IL-8 [Biotin]")
![N/A CXCL8/IL-8 [HRP]](http://images.novusbio.com/fullsize2/DATA_CXCL8_IL8_D8050_ELISA_557.jpg "N/A CXCL8/IL-8 [HRP]")
![N/A CXCL8/IL-8 [HRP]](http://images.novusbio.com/fullsize2/DATA_CXCL8_IL8_D8050_ELISA_558.jpg)
![N/A CXCL8/IL-8 [HRP]](http://images.novusbio.com/fullsize2/CXCL8_D8000C_ELISA_378.jpg)
![Toll-like receptor 4 (TLR4) silencing decreases endogenous levels of pro-inflammatory cytokines. a Effect of TLR2/4 silencing in breast cancer cells transfected with negative control (nc) siRNA, or siRNA directed against TLR2 mRNA (si#1 and si#2) or TLR4 mRNA (si#1 and si#2) was analyzed using quantitative real-time PCR; ***P <0.001 (analysis of variance (ANOVA)). b IL-6 (left) and IL-8 (right) ELISA on supernatants from MDA-MB-231 breast cancer cells transfected with nc siRNA, or siRNA directed against TLR2 mRNA (si#1 and si#2) or TLR4 mRNA (si#1 and si#2); n = 4. Error bars standard error of the mean (SEM); *P <0.05, **P <0.01, ***P <0.001 (ANOVA). c Boyden chamber migration assays. Migration of primary human myeloid cells towards supernatants from different cell lines indicated. Human primary peripheral blood mononuclear cells were isolated as previously described [48] and allowed to migrate through a Costar Transwell® Permeable Support 8.0-μm 24-well plate (Corning) to the supernatants of breast cancer supernatants cultured under serum-free conditions. Percentage of migrated CD11b+ cells was analyzed using a flow cytometer and CD11b-APC antibodies (BD Sciences); n = 4. Error bars SEM; *P <0.05, **P <0.01, ***P <0.001 (ANOVA). d Matrigel invasion assays. Invasion of lipopolysaccharide (LPS)-stimulated/un-stimulated MDA-MB-231 cells into matrigel invasion chambers (BD Sciences) as indicated: 25 × 103 MDA-MB-231 cells were stimulated or not with LPS and allowed to invade from 72 h. Amount of invaded cells was analyzed using crystal violet staining and manual counting in four separate experiments; n = 4. Error bars SEM; *P <0.05, **P <0.01, ***P <0.001 (Student’s t test) Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26392082), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/d8000c_human-il-8-cxcl8-quantikine-elisa-kit-22820241795613.jpg)
![Western Blot: NAP1 Antibody [NBP2-24727] - Western blot analysis of NAP1 in human kidney lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-24727 at 0.5 ug/ml.](http://images.novusbio.com/fullsize/NAP1-Antibody-Western-Blot-NBP2-24727-img0002.jpg "Western Blot: NAP1 Antibody [NBP2-24727] - Western blot analysis of NAP1 in human kidney lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-24727 at 0.5 ug/ml.")
![Western Blot: NAP1 Antibody [NBP1-26592] - Detection of Human and Mouse TAB3 by Western Blot. Samples: Whole cell lysate (50, 15 ug) from 293T and (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TAB3 antibody NBP1-26592 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/NAP1-Antibody-Western-Blot-NBP1-26592-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: NAP1 Antibody [NBP1-26592] - Detection of Human and Mouse TAB3 by Western Blot. Samples: Whole cell lysate (50, 15 ug) from 293T and (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TAB3 antibody NBP1-26592 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: NAP1 Antibody [NBP1-26592] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. TAB3 was also immunoprecipitated by rabbit anti-TAB3 antibody NBP1-26593.](http://images.novusbio.com/fullsize/NAP1-Antibody-Western-Blot-NBP1-26592-img0001.jpg)
![Immunoprecipitation: NAP1 Antibody [NBP1-26592] - Detection of human TAB3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TAB3 antibody NBP1-26592 (lot NBP1-26592-2) used for IP at 6 ug per reaction. TAB3 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-26592-1). For blotting immunoprecipitated TAB3, NBP1-26592 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 60 seconds.](http://images.novusbio.com/fullsize/NAP1-Antibody-Immunoprecipitation-NBP1-26592-img0004.jpg)
![Western Blot: NAP1 Antibody [NBP3-15592] - Western blot analysis of extracts of various cell lines, using NAP1 pAb (NBP3-15592) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/NAP1-Antibody-Western-Blot-NBP3-15592-img0002.jpg "Western Blot: NAP1 Antibody [NBP3-15592] - Western blot analysis of extracts of various cell lines, using NAP1 pAb (NBP3-15592) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: NAP1 Antibody [NBP3-15592] - Immunohistochemistry of paraffin-embedded human liver cancer using NAP1 Rabbit pAb (NBP3-15592) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/NAP1-Antibody-Immunohistochemistry-Paraffin-NBP3-15592-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: NAP1 Antibody [NBP2-33985] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol.](http://images.novusbio.com/fullsize/NAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33985-img0006.jpg "Immunocytochemistry/Immunofluorescence: NAP1 Antibody [NBP2-33985] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol.")
![Immunohistochemistry-Paraffin: NAP1 Antibody [NBP2-33985] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/NAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-33985-img0002.jpg)
![Western Blot: NAP1 Antibody (3Y6T7) [NBP3-15942] - Western blot analysis of extracts of various cell lines, using NAP1 antibody (NBP3-15942) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/NAP1-Antibody-3Y6T7-Western-Blot-NBP3-15942-img0001.jpg "Western Blot: NAP1 Antibody (3Y6T7) [NBP3-15942] - Western blot analysis of extracts of various cell lines, using NAP1 antibody (NBP3-15942) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Western Blot: NAP1 Antibody (3Y6T7) [NBP3-15942] - Western blot analysis of extracts of Mouse liver, using NAP1 antibody (NBP3-15942) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/NAP1-Antibody-3Y6T7-Western-Blot-NBP3-15942-img0002.jpg)
![Western Blot: NAP1 Antibody (SR2283) [NBP3-22101] - Western blot analysis using NBP3-22101 in HepG2 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22101_rabbit-nap1-mab-sr2283-5720231784623.jpg "Western Blot: NAP1 Antibody (SR2283) [NBP3-22101] - Western blot analysis using NBP3-22101 in HepG2 cell lysate.")
![Immunohistochemistry-Paraffin: NAP1 Antibody [NBP1-81262] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/NAP1-Antibody-Immunohistochemistry-Paraffin-NBP1-81262-img0005.jpg "Immunohistochemistry-Paraffin: NAP1 Antibody [NBP1-81262] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.")
![Immunohistochemistry: NAP1 Antibody - BSA Free [NBP2-82062] - Immunohistochemistry of NAP1 in human kidney tissue with NAP1 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-82062_rabbit-polyclonal-nap1-antibody-2711202419313275.jpg "Immunohistochemistry: NAP1 Antibody - BSA Free [NBP2-82062] - Immunohistochemistry of NAP1 in human kidney tissue with NAP1 antibody at 5 ug/mL.")