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![ELISA: SARS-CoV-2 Nucleocapsid Antibody (ARC5077-01-02) - Azide and BSA Free [NBP3-05764] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-ARC5077-01-02-Azide-and-BSA-Free-ELISA-NBP3-05764-img0001.jpg "ELISA: SARS-CoV-2 Nucleocapsid Antibody (ARC5077-01-02) - Azide and BSA Free [NBP3-05764] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.")
![Surface Plasmon Resonance: SARS-CoV-2 Nucleocapsid Antibody (ARC5077-01-02) - Azide and BSA Free [NBP3-05764] - Cat. No. NBP3-05764, captured on COOH chip can bind AB1 with an affinity constant of 1.20nM as determined in a LSPR assay .](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-ARC5077-01-02-Azide-and-BSA-Free-Surface-Plasmon-Resonance-NBP3-05764-img0003.jpg)
![ELISA: SARS-CoV-2 Nucleocapsid Antibody (ARC5077-01-02) - Azide and BSA Free [NBP3-05764] - No cross-reactivity in ELISA with MERS-CoV Nucleoprotein protein and SARS Coronavirus Nucleocapsid.](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-ARC5077-01-02-Azide-and-BSA-Free-ELISA-NBP3-05764-img0002.jpg)
![N/A: SARS-CoV-2 Nucleocapsid Antibody (1C7C7) [NBP3-07043] - Exp1293 cells were transfected with expression plasmid coding for SARS-CoV-2 Nucleocapsid and incubated for 48 hours. Cells were permeabilized, incubated with dilutions of supernatant collected from cells transfected with DNA plasmids coding for the heavy and light chains of 1C7 and binding identified by the addition of goat anti-mouse IgG FITC.](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-1C7C7-N-A-NBP3-07043-img0001.jpg "N/A: SARS-CoV-2 Nucleocapsid Antibody (1C7C7) [NBP3-07043] - Exp1293 cells were transfected with expression plasmid coding for SARS-CoV-2 Nucleocapsid and incubated for 48 hours. Cells were permeabilized, incubated with dilutions of supernatant collected from cells transfected with DNA plasmids coding for the heavy and light chains of 1C7 and binding identified by the addition of goat anti-mouse IgG FITC.")
![Western Blot: SARS-CoV-2 Nucleocapsid Antibody (1C7C7) - Azide and BSA Free [NBP3-07043] - Lysates were derived from A549 cells (Transduced with ACE2 by adenoviral vector), mock vs. SARS-CoV-2-infected MO12, 24 hours, with 60ug of lysates loaded/lane, Image Courtesy of Jeff Johnson Laboratory, ISMMS.](http://images.novusbio.com/fullsize/nbp3-07043_mouse-sars-cov-2-nucleocapsid-mab-1c7c7-azide-bsa-free-271120241937776.jpg)
![Western Blot: SARS Nucleocapsid Protein Antibody (05) [NBP3-06413] - Anti-SARS-NP mouse monoclonal antibody at 1:1000 dilution.Lane A: SARS-CoV NP Protein (30ng)Lane B: SARS-CoV NP Protein (5ng)Lane C: SARS-CoV-2 (2019-nCoV) NP Protein (30ng)Lane D: SARS-CoV-2 (2019-nCoV) NP Protein (5ng)Secondary: Goat Anti-Mouse IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-05-Western-Blot-NBP3-06413-img0001.jpg "Western Blot: SARS Nucleocapsid Protein Antibody (05) [NBP3-06413] - Anti-SARS-NP mouse monoclonal antibody at 1:1000 dilution.Lane A: SARS-CoV NP Protein (30ng)Lane B: SARS-CoV NP Protein (5ng)Lane C: SARS-CoV-2 (2019-nCoV) NP Protein (30ng)Lane D: SARS-CoV-2 (2019-nCoV) NP Protein (5ng)Secondary: Goat Anti-Mouse IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.")
![Western Blot: SARS-CoV-2 Nucleocapsid Antibody (040) [NBP3-12736] - Lane A: SARS-COV-2 Nucleocapsid overexpressed HEK293 Whole Cell LysateLane B: HEK293 Whole Cell LysateLysates/proteins at 10 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing condition](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-040-Western-Blot-NBP3-12736-img0004.jpg "Western Blot: SARS-CoV-2 Nucleocapsid Antibody (040) [NBP3-12736] - Lane A: SARS-COV-2 Nucleocapsid overexpressed HEK293 Whole Cell LysateLane B: HEK293 Whole Cell LysateLysates/proteins at 10 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing condition")
![Western Blot: SARS-CoV-2 Nucleocapsid Antibody (040) [NBP3-12736] - Anti-SARS-NP rabbit monoclonal antibody at 1:1000 dilution.Lane A: SARS-CoV NP Protein Lane B: SARS-CoV NP Protein Lane C: SARS-CoV-2 (2019-nCoV) NP Protein Lane D: SARS-CoV-2 (2019-nCoV) NP Protein SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-040-Western-Blot-NBP3-12736-img0001.jpg)
![Western Blot: SARS-CoV-2 Nucleocapsid Antibody [NBP3-07039] - Coomassie blue staining non-reduced and reduced SDS-PAGE analysis](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-Western-Blot-NBP3-07039-img0001.jpg "Western Blot: SARS-CoV-2 Nucleocapsid Antibody [NBP3-07039] - Coomassie blue staining non-reduced and reduced SDS-PAGE analysis")
![Sandwich ELISA: SARS-CoV-2 Nucleocapsid Antibody [NBP3-07039] - Sandwich ELISA for SARS-CoV-2 Nucleocapsid Matched Pair Antibodies. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (NBP3-07039, 2ug/ml) as capture antibody, a nucleocapsid recombinant protein as the binding protein, and the anti-SARS-CoV-2 Nucleocapsid antibody (NBP3-07068, 0.5ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 15.82 ng](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-Sandwich-ELISA-NBP3-07039-img0002.jpg)
![Western Blot: SARS-CoV-2 Nucleocapsid Antibody (N009) [NBP3-05721] - Western Blot Validation with Recombinant Protein. Loading: 1ug of SARS-CoV-2 (COVID-19) nucleocapsid recombinant protein per lane. Antibodies: SARS-CoV-2 Nucleocapsid Antibody, NBP3-05721, 1:2000. Secondary: Goat anti-human IgG HRP conjugate at 1:5000 dilution.](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-N009-Western-Blot-NBP3-05721-img0002.jpg "Western Blot: SARS-CoV-2 Nucleocapsid Antibody (N009) [NBP3-05721] - Western Blot Validation with Recombinant Protein. Loading: 1ug of SARS-CoV-2 (COVID-19) nucleocapsid recombinant protein per lane. Antibodies: SARS-CoV-2 Nucleocapsid Antibody, NBP3-05721, 1:2000. Secondary: Goat anti-human IgG HRP conjugate at 1:5000 dilution.")
![ELISA: SARS-CoV-2 Nucleocapsid Antibody (N009) [NBP3-05721] - ELISA Coating original concentration: 2 ug/mL, 100 uL/well sample is SARS-CoV-2 Nucleocapsid Recombinant Protein, 100 uL/well. Stop buffer 50 uL/well.](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-N009-ELISA-NBP3-05721-img0001.jpg)
![ELISA: SARS-CoV-2 Nucleocapsid Antibody (NP1189) [NBP3-07071] - Sandwich ELISA for SARS-CoV-2 Nucleocapsid Matched Pair Antibodies. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (NBP3-07069, 2 ug/mL) as capture antibody, a Nucleocapsid recombinant protein as the binding protein, and the anti-SARS-CoV-2 Nucleocapsid antibody (NBP3-07071, 0.1 ug/mL) as the detection antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 6.9 ng.](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-NP1189-Sandwich-ELISA-NBP3-07071-img0001.jpg "ELISA: SARS-CoV-2 Nucleocapsid Antibody (NP1189) [NBP3-07071] - Sandwich ELISA for SARS-CoV-2 Nucleocapsid Matched Pair Antibodies. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (NBP3-07069, 2 ug/mL) as capture antibody, a Nucleocapsid recombinant protein as the binding protein, and the anti-SARS-CoV-2 Nucleocapsid antibody (NBP3-07071, 0.1 ug/mL) as the detection antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 6.9 ng.")
![Immunohistochemistry: SARS-CoV-2 Nucleocapsid Antibody (NP1189) - Azide and BSA Free [NBP3-07071] - Validation of SARS-CoV-2 (COVID-19) Nucleocapsid in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Nucleocapsid antibody (1 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Nucleocapsid protein was observed in the macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.](http://images.novusbio.com/fullsize/nbp3-07071_rabbit-monoclonal-sars-cov-2-nucleocapsid-antibody-np1189-2711202420272423.jpg)
![Sandwich ELISA: SARS-CoV-2 Nucleocapsid Antibody (3868) [NBP3-07070] - Sandwich ELISA for SARS-CoV-2 Nucleocapsid Matched Pair Antibodies. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (NBP3-07038, 2ug/ml) as capture antibody, a nucleocapsid recombinant protein as the binding protein, and the anti-SARS-CoV-2 Nucleocapsid antibody (NBP3-07070, 0.5ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 4.27 ng](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-3868-Sandwich-ELISA-NBP3-07070-img0001.jpg "Sandwich ELISA: SARS-CoV-2 Nucleocapsid Antibody (3868) [NBP3-07070] - Sandwich ELISA for SARS-CoV-2 Nucleocapsid Matched Pair Antibodies. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (NBP3-07038, 2ug/ml) as capture antibody, a nucleocapsid recombinant protein as the binding protein, and the anti-SARS-CoV-2 Nucleocapsid antibody (NBP3-07070, 0.5ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 4.27 ng")
![Sandwich ELISA: SARS-CoV-2 Nucleocapsid Antibody (3868) [NBP3-07070] - Sandwich ELISA for SARS-CoV-2 Nucleocapsid Antibodies. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (NBP3-07039, 2ug/ml) as capture antibody, the Nucleocapsid recombinant protein as the binding protein, and the anti-SARS-CoV-2 Nucleocapsid antibody (NBP3-07068, 0.5ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 15.82 ng](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-3868-Sandwich-ELISA-NBP3-07070-img0002.jpg)
![ELISA: SARS-CoV-2 Nucleocapsid Antibody (75G5a) [NBP3-07040] - Microtiter wells were coated with SARS-CoV-2 Nucleocapsid and SARS-CoV Nucleocapsid at 1 ug/mL. Purified rabbit monoclonal antibodies were serially diluted 1:2 starting at 1 ug/mL, which showed very strong and specific binding to COVID-19 Nucleocapsid antigen, with no significant cross-reactivity to SARS-CoV Nucleocapsid antigen.](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-75G5a-ELISA-NBP3-07040-img0001.jpg "ELISA: SARS-CoV-2 Nucleocapsid Antibody (75G5a) [NBP3-07040] - Microtiter wells were coated with SARS-CoV-2 Nucleocapsid and SARS-CoV Nucleocapsid at 1 ug/mL. Purified rabbit monoclonal antibodies were serially diluted 1:2 starting at 1 ug/mL, which showed very strong and specific binding to COVID-19 Nucleocapsid antigen, with no significant cross-reactivity to SARS-CoV Nucleocapsid antigen.")
![Sandwich ELISA: SARS-CoV-2 Nucleocapsid Antibody (75G5a) [NBP3-07040] - A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid specific rabbit monoclonal antibodies: NBP3-07040 as a capture antibody and a biotin-conjugated antibody as a detection antibody. SARS-CoV-2 Nucleocapsid was serially diluted 1:2 starting at 25 ng/mL. Rabbit monoclonal antibodies NBP3-07040 and a biotin-conjugated antibody detected SARS-CoV-2 Nucleocapsid antigen at very high sensitivity as low as 100 pg/mL (100 pg).](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-75G5a-Sandwich-ELISA-NBP3-07040-img0002.jpg)
![Sandwich ELISA: SARS-CoV-2 Nucleocapsid Antibody (75G5a) [NBP3-07040] - A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid specific rabbit monoclonal antibodies: NBP3-07040 as a capture antibody and a biotin-conjugated antibody as a detection antibody. SARS-CoV-2 Nucleocapsid was serially diluted 1:2 starting at 25 ng/mL. Rabbit monoclonal antibodies NBP3-07040 and a biotin-conjugated antibody detected SARS-CoV-2 Nucleocapsid antigen at very high sensitivity as low as 25 pg/mL (25 pg).](http://images.novusbio.com/fullsize/SARS-CoV-2-Nucleocapsid-Antibody-75G5a-Sandwich-ELISA-NBP3-07040-img0003.jpg)
