SARS Nucleocapsid Protein Antibody (8G8A) - Azide and BSA Free

Western Blot: SARS Nucleocapsid Protein Antibody (8G8A) [NBP3-07064] - Western Blot Validation of SARS Nucleocapsid Antibody. Lane 1: SARS-CoV-2 Nucleocapsid protein, Lane 2: recombinant SARS Nucleocapsid Protein and Lane 3: MERS Nucleocapsid protein probed with SARS Nucleocapsid Antibody [8G8A], NBP3-07064, at 1:1000 dilution and 4C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37C.

• Reactivity: V
• Applications: WB, ELISA
• Clone: 8G8A
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

SARS Nucleocapsid Protein Antibody (8G8A) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse SARS Nucleocapsid Protein Antibody (8G8A) - Azide and BSA Free (NBP3-07064) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant SARS Nucleocapsid Protein (no tag)
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: N
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1000
  • Western Blot 1:500-2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.01M PBS (pH 7.4)
• Preservative: 0.1% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for SARS Nucleocapsid Protein Antibody (8G8A) - Azide and BSA Free

• N protein
• N structural protein
• N
• NC
• Nucleocapsid protein
• Nucleoprotein
• Protein N
• SARS coronavirus N protein
• SARS coronavirus nucleocapsid protein
• SARS CoV N protein
• SARS CoV nucleocapsid protein
• SARS CoV
• SARS N protein
• SARS Nucleoprotein
• SARSCoV N protein
• SARSCoV nucleocapsid protein
• SARSCoV
• Severe acute respiratory syndrome

Background

It has recently been shown that SARS is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139 kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: N