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![Western Blot: MLF1 Interacting Protein [p Thr78] Antibody [NBP1-77938] - Shows detection of MLF1IP phosphorylated at Thr78. HeLa cells were co-infected with the indicated adenoviruses expressing GFP-tagged Plk1 or PBIP1. Blots were probed with the anti-MLF1IP pT78 antibody, stripped, and then reprobed with anti-GFP antibody (KA/G & Park, et al., 2006).](http://images.novusbio.com/fullsize/MLF1-Interacting-Protein-[p-Thr78]-Antibody-Western-Blot-NBP1-77938-img0004.jpg "Western Blot: MLF1 Interacting Protein [p Thr78] Antibody [NBP1-77938] - Shows detection of MLF1IP phosphorylated at Thr78. HeLa cells were co-infected with the indicated adenoviruses expressing GFP-tagged Plk1 or PBIP1. Blots were probed with the anti-MLF1IP pT78 antibody, stripped, and then reprobed with anti-GFP antibody (KA/G & Park, et al., 2006).")
![Immunohistochemistry: MLF1 Interacting Protein [p Thr78] Antibody [NBP1-77938] - Used at 20 g/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderately positive staining of mitotic cells in colon adenocarcinoma at 60X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.](http://images.novusbio.com/fullsize/MLF1-Interacting-Protein-[p-Thr78]-Antibody-Immunohistochemistry-NBP1-77938-img0007.jpg)
![Immunohistochemistry: MLF1 Interacting Protein [p Thr78] Antibody [NBP1-77938] - Analysis of MLF1IP pT78 at the kinetochores of HeLa cells in different phases of the cell cycle. Fluorescent signals were detectable at the kinetochores as early as G2, became most abundant in prophase cells with a discernible nuclear envelope, and gradually diminished as cells proceeded through mitosis (Kang & Park, et al., 2006).](http://images.novusbio.com/fullsize/MLF1-Interacting-Protein-[p-Thr78]-Antibody-Immunohistochemistry-NBP1-77938-img0006.jpg)
![Western Blot: MLF1 Interacting Protein Antibody - N-terminal [NB110-85541] - Analysis of endogenous MLF1IP protein (a tier of four modified protein bands indicated by the arrowheads) in lysates of Hela cells (- lane). Cells treated with MLF1IP / PBIP1 shRNA (+ lane) show no staining. The identities of the higher and lower molecular weight bands are unknown. Primary antibody was used at 1:1,000.](http://images.novusbio.com/fullsize/MLF1-Interacting-Protein-Antibody---N-terminal-Western-Blot-NB110-85541-img0007.jpg "Western Blot: MLF1 Interacting Protein Antibody - N-terminal [NB110-85541] - Analysis of endogenous MLF1IP protein (a tier of four modified protein bands indicated by the arrowheads) in lysates of Hela cells (- lane). Cells treated with MLF1IP / PBIP1 shRNA (+ lane) show no staining. The identities of the higher and lower molecular weight bands are unknown. Primary antibody was used at 1:1,000.")
![Immunohistochemistry: MLF1 Interacting Protein Antibody - N-terminal [NB110-85541] - Tissue: heart. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-MLF1 at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: MLF1 as precipitated red signal with hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/MLF1-Interacting-Protein-Antibody---N-terminal-Immunohistochemistry-NB110-85541-img0005.jpg)
![Immunohistochemistry: MLF1 Interacting Protein Antibody - N-terminal [NB110-85541] - Tissue: brain, cerebellum. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-MLF1 at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: MLF1 as precipitated red signal with hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/MLF1-Interacting-Protein-Antibody---N-terminal-Immunohistochemistry-NB110-85541-img0004.jpg)
