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![ELISA: Rat Tau ELISA Kit (Colorimetric) [NBP2-81164] - Samples were spiked with high concentrations of Rat Tau and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-81164_rat-tau-elisa-kit-colorimetric-13220241617342.jpg "ELISA: Rat Tau ELISA Kit (Colorimetric) [NBP2-81164] - Samples were spiked with high concentrations of Rat Tau and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Tau ELISA Kit (Colorimetric) [NBP2-81164] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-81164_rat-tau-elisa-kit-colorimetric-4320248582587.jpg)
![Western Blot: Rabbit Tau [p Thr217] mAb (HL1276) - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (p Thr217) antibody [HL1276] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45156_rabbit-tau-p-thr217-mab-hl1276-azide-and-bsa-free-western-blot-2942025165210..jpg "Western Blot: Rabbit Tau [p Thr217] mAb (HL1276) - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (p Thr217) antibody [HL1276] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![ELISA: Rabbit Tau [p Thr217] mAb (HL1276) [NBP3-45156] - Indirect ELISA analysis was performed by coating the plate with Tau peptide and Tau (p Thr217) peptide (1000-15.63 ng/mL). Coated peptides were probed with Tau (p Thr217) antibody [HL1276] (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-45156_rabbit-tau-p-thr217-mab-hl1276-azide-and-bsa-free-elisa-2942025164816..jpg)
![ELISA: Rat Tau [p Ser610] ELISA Kit (Colorimetric) [NBP2-76716] - Standard Curve Reference](http://images.novusbio.com/fullsize/Rat-Tau-p-Ser610-ELISA-Kit-Colorimetric-ELISA-NBP2-76716-img0001.jpg "ELISA: Rat Tau [p Ser610] ELISA Kit (Colorimetric) [NBP2-76716] - Standard Curve Reference")
![ELISA: Rat Tau [p Ser610] ELISA Kit (Colorimetric) [NBP2-76716] - Samples were spiked with high concentrations of Rat Tau and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76716_rat-tau-p-ser610-elisa-kit-colorimetric-132202416235753.jpg)
![ELISA: Rat Tau [p Ser610] ELISA Kit (Colorimetric) [NBP2-76716] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76716_rat-tau-p-ser610-elisa-kit-colorimetric-432024903597.jpg)
![ELISA: Mouse Tau ELISA Kit (Colorimetric) [NBP2-81260] - Samples were spiked with high concentrations of Mouse Tau and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-81260_mouse-tau-elisa-kit-colorimetric-132202416232385.jpg "ELISA: Mouse Tau ELISA Kit (Colorimetric) [NBP2-81260] - Samples were spiked with high concentrations of Mouse Tau and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Tau ELISA Kit (Colorimetric) [NBP2-81260] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-81260_mouse-tau-elisa-kit-colorimetric-432024855058.jpg)
![Western Blot: Tau [p Ser202, p Ser199, p Ser198] Antibody [NBP3-16924] - Western blot analysis of extracts of NIH/3T3 cells, using Tau pAb (NBP3-16924) at 1:1000 dilution or Tau antibody.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 120s.](http://images.novusbio.com/fullsize/Tau-[p-Ser202-p-Ser199-p-Ser198]-Antibody-Western-Blot-NBP3-16924-img0001.jpg "Western Blot: Tau [p Ser202, p Ser199, p Ser198] Antibody [NBP3-16924] - Western blot analysis of extracts of NIH/3T3 cells, using Tau pAb (NBP3-16924) at 1:1000 dilution or Tau antibody.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 120s.")
![Immunohistochemistry: Tau [p Ser199] Antibody (23E8) [NBP3-26411] - Image of Tau [p Ser199] Antibody (23E8) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.10% DAB.](http://images.novusbio.com/fullsize/nbp3-26411_rabbit-tau-p-ser-199-mab-23e8-27220248471916.jpg "Immunohistochemistry: Tau [p Ser199] Antibody (23E8) [NBP3-26411] - Image of Tau [p Ser199] Antibody (23E8) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.10% DAB.")
![Flow Cytometry: Tau [p Ser199] Antibody (23E8) [NBP3-26411] - Overlay Peak curve showing SH-SY5Y cells stained with Tau [p Ser199] Antibody (23E8) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26411_rabbit-tau-p-ser-199-mab-23e8-282202414183638.jpg)
![Western Blot: Tau Antibody (S09-5E2) [NBP3-20020] - Western blot detection of Tau in Rat Brain cell using NBP3-20020 (1:1000 diluted). Predicted band size: 79kDa. Observed band size: 50-80kDa.](http://images.novusbio.com/fullsize/Tau-Antibody-S09-5E2-Western-Blot-NBP3-20020-img0001.jpg "Western Blot: Tau Antibody (S09-5E2) [NBP3-20020] - Western blot detection of Tau in Rat Brain cell using NBP3-20020 (1:1000 diluted). Predicted band size: 79kDa. Observed band size: 50-80kDa.")
![Western Blot: Tau Antibody (S03-9I2) [NBP3-20059] - Western blot detection of Tau in Human Hippocampus lysates using NBP3-20059 (1:1000 diluted). Predicted band size: 79KDa. Observed band size: 50-80KDa.](http://images.novusbio.com/fullsize/Tau-Antibody-S03-9I2-Western-Blot-NBP3-20059-img0001.jpg "Western Blot: Tau Antibody (S03-9I2) [NBP3-20059] - Western blot detection of Tau in Human Hippocampus lysates using NBP3-20059 (1:1000 diluted). Predicted band size: 79KDa. Observed band size: 50-80KDa.")
![Western Blot: Tau [p Ser202] Antibody (S01-5A7) [NBP3-20058] - Western blot detection of Tau in Rat Brain lysates using NBP3-20058 (1:1000 diluted). Predicted band size: 79kDa. Observed band size: 50-80kDa.](http://images.novusbio.com/fullsize/Tau-p-Ser202-Antibody-S01-5A7-Western-Blot-NBP3-20058-img0001.jpg "Western Blot: Tau [p Ser202] Antibody (S01-5A7) [NBP3-20058] - Western blot detection of Tau in Rat Brain lysates using NBP3-20058 (1:1000 diluted). Predicted band size: 79kDa. Observed band size: 50-80kDa.")
![Western Blot: Tau [p Ser214] Antibody (JE66-83) [NBP3-32805] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32805) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 79 kDa Observed band size: 40-55 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32805) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32805_rabbit-tau-p-ser214-mab-je66-83-288202412442910.jpg "Western Blot: Tau [p Ser214] Antibody (JE66-83) [NBP3-32805] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32805) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 79 kDa Observed band size: 40-55 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32805) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Tau [p Ser198] Antibody (JE66-79) [NBP3-32801] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32801) at 1/2,000 dilution. Lane 1: Mouse hippocampus tissue lysate Lane 2: Rat hippocampus tissue lysate Lane 3: Mouse hippocampus treated with pp for 1 hour tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32801) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32801_rabbit-tau-p-ser198-mab-je66-79-288202412442947.jpg "Western Blot: Tau [p Ser198] Antibody (JE66-79) [NBP3-32801] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32801) at 1/2,000 dilution. Lane 1: Mouse hippocampus tissue lysate Lane 2: Rat hippocampus tissue lysate Lane 3: Mouse hippocampus treated with pp for 1 hour tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32801) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")