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![Immunocytochemistry/Immunofluorescence: ERK2 Antibody (PCRP-MAPK1-1D1) [NBP3-13754] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained usingERK2 Antibody (PCRP-MAPK1-1D1)followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.](http://images.novusbio.com/fullsize/ERK2-Antibody-PCRP-MAPK1-1D1-Immunocytochemistry-Immunofluorescence-NBP3-13754-img0002.jpg "Immunocytochemistry/Immunofluorescence: ERK2 Antibody (PCRP-MAPK1-1D1) [NBP3-13754] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained usingERK2 Antibody (PCRP-MAPK1-1D1)followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.")
![Flow Cytometry: ERK2 Antibody (PCRP-MAPK1-1D1) [NBP3-13754] - Flow cytometric analysis of PFA-fixed HeLa cells. ERK2 antibody (PCRP-MAPK1-1D1) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).](http://images.novusbio.com/fullsize/ERK2-Antibody-PCRP-MAPK1-1D1-Flow-Cytometry-NBP3-13754-img0001.jpg)
![Protein Array: ERK2 Antibody (PCRP-MAPK1-1D1) [NBP3-13754] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ERK2 Antibody (PCRP-MAPK1-1D1).](http://images.novusbio.com/fullsize/ERK2-Antibody-PCRP-MAPK1-1D1-Protein-Array-NBP3-13754-img0004.jpg)
![Western Blot: ERK2 Antibody (RM483) [NBP3-23465] - Western Blot of 293 cell lysate using Clone RM483) at 1:2000 dilution.](http://images.novusbio.com/fullsize/nbp3-23465_rabbit-erk2-mab-246202413261.png "Western Blot: ERK2 Antibody (RM483) [NBP3-23465] - Western Blot of 293 cell lysate using Clone RM483) at 1:2000 dilution.")
![Immunohistochemistry-Paraffin: ERK2 Antibody (RM483) [NBP3-23465] - Immunohistochemical staining of formalin fixed and paraffin embedded human Lung Squamous Carcinoma tissue sections using Clone RM483) at 1:100 dilution.](http://images.novusbio.com/fullsize/nbp3-23465_rabbit-erk2-mab-2462024132636.png)
![Immunohistochemistry-Paraffin: ERK2 Antibody (RM483) [NBP3-23465] - Immunohistochemical staining of formalin fixed and paraffin embedded human Brain tissue sections using Clone RM483) at 1:100 dilution.](http://images.novusbio.com/fullsize/nbp3-23465_rabbit-erk2-mab-226202422321232.png)
![Western Blot: ERK2 Antibody [NBP3-02973] - Western blot analysis of extracts from normal (control) and ERK2 knockout (KO) HeLa cells, using ERK2 antibody (NBP3-02973) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/ERK2-Antibody-Western-Blot-NBP3-02973-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ERK2 Antibody [NBP3-02973] - Western blot analysis of extracts from normal (control) and ERK2 knockout (KO) HeLa cells, using ERK2 antibody (NBP3-02973) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![<b>Genetic Strategies Validation. </b>Western Blot: ERK2 Antibody [NBP3-02973] - Western blot analysis of extracts of various cell lines, using ERK2 antibody (NBP3-02973) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/ERK2 Antibody-Western Blot-NBP3-02973-img0002.jpg)
![SDS-Page: Recombinant Human ERK2 Protein [NBP1-30309] - 15% SDS-Page (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-ERK2-Protein-SDS-Page-NBP1-30309-img0001.jpg "SDS-Page: Recombinant Human ERK2 Protein [NBP1-30309] - 15% SDS-Page (3ug)")
![Protein Array: ERK2 Antibody (PCRP-MAPK1-1D1) - Azide and BSA Free [NBP3-14157] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ERK2 Antibody (PCRP-MAPK1-1D1).](http://images.novusbio.com/fullsize/ERK2-Antibody-PCRP-MAPK1-1D1-Azide-and-BSA-Free-Protein-Array-NBP3-14157-img0001.jpg "Protein Array: ERK2 Antibody (PCRP-MAPK1-1D1) - Azide and BSA Free [NBP3-14157] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ERK2 Antibody (PCRP-MAPK1-1D1).")
![Western Blot: ERK2 Knockout A549 Cell Lysate [NBP3-18632] - Lane 1: Opal Prestained MW Marker Lane 2: A549 WCL Parental. Lane 3: A549 MAPK1 KO Clone 4. Lane 4: A549 MAPK1 KO Clone 10. Lane 5: A549 MAPK1 KO Clone 15. Lane 6: HeLa WCL Parental Load: 10ug lysate/lane. Primary Antibody [Blot A] Anti-MAPK1(ERK2) ~44kDa; [Blot B] stripped and re-probed with Anti-Tubulin ~50kDa; at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:30,000 for 1hr at RT. Block: BlockOut Buffer for 1hr at RT. No detection of expected band at ~44kDa is observed in MAPK1 (ERK2) knockout A549 when compared with unmodified A549 cell lysates by Western blot.](http://images.novusbio.com/fullsize/ERK2-Knockout-A549-Cell-Lysate-Western-Blot-NBP3-18632-img0001.jpg "Western Blot: ERK2 Knockout A549 Cell Lysate [NBP3-18632] - Lane 1: Opal Prestained MW Marker Lane 2: A549 WCL Parental. Lane 3: A549 MAPK1 KO Clone 4. Lane 4: A549 MAPK1 KO Clone 10. Lane 5: A549 MAPK1 KO Clone 15. Lane 6: HeLa WCL Parental Load: 10ug lysate/lane. Primary Antibody [Blot A] Anti-MAPK1(ERK2) ~44kDa; [Blot B] stripped and re-probed with Anti-Tubulin ~50kDa; at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:30,000 for 1hr at RT. Block: BlockOut Buffer for 1hr at RT. No detection of expected band at ~44kDa is observed in MAPK1 (ERK2) knockout A549 when compared with unmodified A549 cell lysates by Western blot.")
![Western Blot: ERK2 Antibody (5-D2-R) [NBP3-32317] - Western blot analysis of ERK2 on different lysates with Mouse anti-ERK2 antibody (NBP3-32317) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: A549 cell lysate (20 ug/Lane) Lane 4: A431 cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: HEK-293 cell lysate (20 ug/Lane) Lane 7: NIH/3T3 cell lysate (20 ug/Lane) Lane 8: RAW264.7 cell lysate (20 ug/Lane) Lane 9: C6 cell lysate (20 ug/Lane) Lane 10: PC-12 cell lysate (20 ug/Lane) Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32317) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Alpaca Anti-Mouse IgG - HRP for IP Nano-Secondary Antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32317_mouse-erk2-mab-5-d2-r-278202415295598.jpg "Western Blot: ERK2 Antibody (5-D2-R) [NBP3-32317] - Western blot analysis of ERK2 on different lysates with Mouse anti-ERK2 antibody (NBP3-32317) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: A549 cell lysate (20 ug/Lane) Lane 4: A431 cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: HEK-293 cell lysate (20 ug/Lane) Lane 7: NIH/3T3 cell lysate (20 ug/Lane) Lane 8: RAW264.7 cell lysate (20 ug/Lane) Lane 9: C6 cell lysate (20 ug/Lane) Lane 10: PC-12 cell lysate (20 ug/Lane) Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32317) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Alpaca Anti-Mouse IgG - HRP for IP Nano-Secondary Antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.")
![Western Blot: ERK2 Antibody (C3B12-R) [NBP3-32273] - Western blot analysis of ERK2 on different lysates with Mouse anti-ERK2 antibody (NBP3-32273) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: A549 cell lysate (20 ug/Lane) Lane 4: A431 cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: HEK-293 cell lysate (20 ug/Lane) Lane 7: NIH/3T3 cell lysate (20 ug/Lane) Lane 8: RAW264.7 cell lysate (20 ug/Lane) Lane 9: C6 cell lysate (20 ug/Lane) Lane 10: Human brain tissue lysate (40 ug/Lane) Lane 11: Mouse brain tissue lysate (40 ug/Lane) Lane 12: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32273) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Alpaca Anti-Mouse IgG - HRP for IP Nano-Secondary Antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32273_mouse-erk2-mab-c3b12-r-278202415242691.jpg "Western Blot: ERK2 Antibody (C3B12-R) [NBP3-32273] - Western blot analysis of ERK2 on different lysates with Mouse anti-ERK2 antibody (NBP3-32273) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: A549 cell lysate (20 ug/Lane) Lane 4: A431 cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: HEK-293 cell lysate (20 ug/Lane) Lane 7: NIH/3T3 cell lysate (20 ug/Lane) Lane 8: RAW264.7 cell lysate (20 ug/Lane) Lane 9: C6 cell lysate (20 ug/Lane) Lane 10: Human brain tissue lysate (40 ug/Lane) Lane 11: Mouse brain tissue lysate (40 ug/Lane) Lane 12: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32273) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Alpaca Anti-Mouse IgG - HRP for IP Nano-Secondary Antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.")
![Western Blot: ERK2 Overexpression Lysate (Adult Normal) [NBL1-12866] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ERK2.](http://images.novusbio.com/fullsize/ERK2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12866-img0002.jpg "Western Blot: ERK2 Overexpression Lysate (Adult Normal) [NBL1-12866] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ERK2.")
![Western Blot: ERK2 Overexpression Lysate (Adult Normal) [NBL1-12865] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ERK2.](http://images.novusbio.com/fullsize/ERK2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12865-img0002.jpg "Western Blot: ERK2 Overexpression Lysate (Adult Normal) [NBL1-12865] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ERK2.")