NL012) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="LRP-1 Cluster III was detected in immersion fixed MG-63 human osteosarcoma cell line using Sheep Anti-Human LRP-1 Cluster III Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4824) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green; NL012) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4824-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-62753-0.025mg | |||
![ELISA: Mouse LRP-1 ELISA Kit (Colorimetric) [NBP3-00449] - Samples were spiked with high concentrations of Mouse LRP-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-00449_mouse-lrp-1-elisa-kit-colorimetric-132202416235752.jpg "ELISA: Mouse LRP-1 ELISA Kit (Colorimetric) [NBP3-00449] - Samples were spiked with high concentrations of Mouse LRP-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse LRP-1 ELISA Kit (Colorimetric) [NBP3-00449] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-00449_mouse-lrp-1-elisa-kit-colorimetric-43202495503.jpg)
![Western Blot: LRP-1 Antibody [NBP2-62753] - Western blot analysis of Mouse, Rat Brain showing detection of 504.6 kDa LRP-1 protein using Rabbit Anti-LRP-1 Polyclonal Antibody (NBP2-62753). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Lane 3: Rat Brain . Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-LRP-1 Polyclonal Antibody (NBP2-62753) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 504.6 kDa. Other Band(s): 80 kDa.](http://images.novusbio.com/fullsize/LRP-1-Antibody-Western-Blot-NBP2-62753-img0003.jpg "Western Blot: LRP-1 Antibody [NBP2-62753] - Western blot analysis of Mouse, Rat Brain showing detection of 504.6 kDa LRP-1 protein using Rabbit Anti-LRP-1 Polyclonal Antibody (NBP2-62753). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Lane 3: Rat Brain . Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-LRP-1 Polyclonal Antibody (NBP2-62753) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 504.6 kDa. Other Band(s): 80 kDa.")
![Immunohistochemistry: LRP-1 Antibody [NBP2-62753] - Immunohistochemistry analysis using Rabbit Anti-LRP1 Polyclonal Antibody (NBP2-62753). Tissue: Liver. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-LRP1 Polyclonal Antibody (NBP2-62753) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.](http://images.novusbio.com/fullsize/LRP-1 Antibody-Immunohistochemistry-NBP2-62753-img0004.jpg)
![Western Blot: LRP-1 Antibody (4O3Y8) [NBP3-15289] - Western blot analysis of extracts of various cell lines, using LRP-1 antibody (NBP3-15289) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/LRP-1-Antibody-4O3Y8-Western-Blot-NBP3-15289-img0001.jpg "Western Blot: LRP-1 Antibody (4O3Y8) [NBP3-15289] - Western blot analysis of extracts of various cell lines, using LRP-1 antibody (NBP3-15289) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![ELISA: Human LRP-1 ELISA Kit (Colorimetric) [NBP3-40028] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40028_human-lrp-1-elisa-kit-colorimetric-2110202415264390.png "ELISA: Human LRP-1 ELISA Kit (Colorimetric) [NBP3-40028] - Standard Curve Reference")
