| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-35228-10ug | |||
| Carrier-Free | NBP2-35228-1mg | |||
| Carrier-Free | NBP2-35228-500ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-35307-10ug | |||
| Carrier-Free | NBP2-35307-1000ug | |||
| Carrier-Free | NBP2-35307-500ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AFL201-025 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3340-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB8049-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 6226-GL-010/CF | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-11364-0.025mg | |||
![SDS-Page: Porcine IL-1 beta/IL-1F2 Protein [NBP2-35228]](http://images.novusbio.com/fullsize/Porcine-IL-1-beta-IL-1F2-Protein-SDS-Page-NBP2-35228-img0001.jpg "SDS-Page: Porcine IL-1 beta/IL-1F2 Protein [NBP2-35228]")
![SDS-Page: Primate IL-1 beta/IL-1F2 Protein [NBP2-35307]](http://images.novusbio.com/fullsize/Primate-IL-1-beta-IL-1F2-Protein-SDS-Page-NBP2-35307-img0001.jpg "SDS-Page: Primate IL-1 beta/IL-1F2 Protein [NBP2-35307]")
![ELISA: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - IL-1 beta/IL-1F2 Antibody (NBP3-32464) in indirect ELISA. Indirect ELISA analysis of IL-1 beta was performed by coating wells of a 96-well plate with 50 µl per well of IL-1 beta antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with blocking buffer, and incubated with 50 µl per well of a mouse IL-1 beta monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-27820241141613.jpg "ELISA: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - IL-1 beta/IL-1F2 Antibody (NBP3-32464) in indirect ELISA. Indirect ELISA analysis of IL-1 beta was performed by coating wells of a 96-well plate with 50 µl per well of IL-1 beta antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with blocking buffer, and incubated with 50 µl per well of a mouse IL-1 beta monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.")
![Western Blot: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - Western blot analysis of IL-1 beta/IL-1F2 on different lysates with Mouse anti-IL-1 beta/IL-1F2 antibody (NBP3-32464) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours add 300ng/mL BFA for 3 hours cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32464) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-27820241631654.jpg)
![Immunohistochemistry: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-IL-1 beta/IL-1F2 antibody (NBP3-32464) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32464) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-29820241420948.jpg)
![Western Blot: IL1 beta Overexpression Lysate (Adult Normal) [NBP2-04165] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IL1 beta.](http://images.novusbio.com/fullsize/IL1-beta-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04165-img0001.jpg "Western Blot: IL1 beta Overexpression Lysate (Adult Normal) [NBP2-04165] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IL1 beta.")
![ELISA: IL-1 beta/IL-1F2 Antibody [NBP3-11364] - Recombinant Rabbit IL-1 beta/IL-1F2 detected using Chicken anti Rabbit IL-1 beta/IL-1F2 as the capture reagent and Chicken anti Rabbit IL-1 beta/IL-1F2 beta:Biotin as the detection reagent followed by Streptavidin:HRP.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-ELISA-NBP3-11364-img0001.jpg "ELISA: IL-1 beta/IL-1F2 Antibody [NBP3-11364] - Recombinant Rabbit IL-1 beta/IL-1F2 detected using Chicken anti Rabbit IL-1 beta/IL-1F2 as the capture reagent and Chicken anti Rabbit IL-1 beta/IL-1F2 beta:Biotin as the detection reagent followed by Streptavidin:HRP.")
![Western Blot: IL-1 beta/IL-1F2 Antibody (PS01-39) [NBP3-32449] - Western blot analysis of IL-1 beta/IL-1F2 on different lysates with Rabbit anti-IL-1 beta/IL-1F2 antibody (NBP3-32449) at 1/1,000 dilution. Lane 1: THP-1 whole cell lysate Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours and 300ng/mL BFA for 3 hours whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 31 kDa Observed band size: 31/28 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32449) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32449_rabbit-il-1-beta-il-1f2-mab-ps01-39-278202415212445.jpg "Western Blot: IL-1 beta/IL-1F2 Antibody (PS01-39) [NBP3-32449] - Western blot analysis of IL-1 beta/IL-1F2 on different lysates with Rabbit anti-IL-1 beta/IL-1F2 antibody (NBP3-32449) at 1/1,000 dilution. Lane 1: THP-1 whole cell lysate Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours and 300ng/mL BFA for 3 hours whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 31 kDa Observed band size: 31/28 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32449) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")