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![ELISA: Mouse GRP78/HSPA5 ELISA Kit (Colorimetric) [NBP2-82202] - Samples were spiked with high concentrations of Mouse GRP78/HSPA5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-82202_mouse-grp78-hspa5-elisa-kit-colorimetric-13220241620403.jpg "ELISA: Mouse GRP78/HSPA5 ELISA Kit (Colorimetric) [NBP2-82202] - Samples were spiked with high concentrations of Mouse GRP78/HSPA5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse GRP78/HSPA5 ELISA Kit (Colorimetric) [NBP2-82202] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-82202_mouse-grp78-hspa5-elisa-kit-colorimetric-4320249151613.jpg)
![Western Blot: GRP78 Overexpression Lysate (Adult Normal) [NBP2-10471] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GRP78.](http://images.novusbio.com/fullsize/GRP78-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10471-img0001.jpg "Western Blot: GRP78 Overexpression Lysate (Adult Normal) [NBP2-10471] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GRP78.")
![SDS-Page: Recombinant Human GRP78/HSPA5 His Protein [NBC1-18378] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant-Human-GRP78-HSPA5-His-Protein-SDS-Page-NBC1-18378-img0003.jpg "SDS-Page: Recombinant Human GRP78/HSPA5 His Protein [NBC1-18378] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.")
![SDS-Page: Recombinant Mouse GRP78/HSPA5 Protein [NBP2-61186] - 15 % SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-Fc-gamma-RIIIB-CD16b-Protein-SDS-Page-NBP2-61186-img0001.jpg "SDS-Page: Recombinant Mouse GRP78/HSPA5 Protein [NBP2-61186] - 15 % SDS-PAGE (3ug)")
![Immunohistochemistry: GRP78/HSPA5 Antibody (4H12) [NBP3-15138] - IHC image of GRP78/HSPA5 antibody diluted at 1:100 and staining in paraffin-embedded human breast cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-4H12-Immunohistochemistry-NBP3-15138-img0001.jpg "Immunohistochemistry: GRP78/HSPA5 Antibody (4H12) [NBP3-15138] - IHC image of GRP78/HSPA5 antibody diluted at 1:100 and staining in paraffin-embedded human breast cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![SDS-Page: Recombinant Human GRP78/HSPA5 Protein [NBP1-50908] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant-Human-GRP78-HSPA5-Protein-SDS-Page-NBP1-50908-img0002.jpg "SDS-Page: Recombinant Human GRP78/HSPA5 Protein [NBP1-50908] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.")
![SDS-Page: Recombinant Human GRP78/HSPA5 His Protein [NBP2-23104] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant-Human-GRP78-HSPA5-His-Protein-SDS-Page-NBP2-23104-img0002.jpg "SDS-Page: Recombinant Human GRP78/HSPA5 His Protein [NBP2-23104] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.")
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP1-89968] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP1-89968-img0013.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP1-89968] - Staining of human lymph node shows strong cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP1-89968-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: GRP78/HSPA5 Antibody [NBP1-89968] - Analysis using Anti-HSPA5 antibody NBP1-89968 (A) shows similar pattern to independent antibody NBP1-89969 (B).](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP1-89968-img0011.jpg)
![ELISA: Human, Mouse, Rat GRP78/HSPA5 ELISA Kit (Colorimetric) [NBP2-62145] - A typical standard curve for GRP78/HSPA5 ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.](http://images.novusbio.com/fullsize/Human-Mouse-Rat-GRP78-HSPA5-ELISA-Kit-Colorimetric-ELISA-NBP2-62145-img0001.jpg "ELISA: Human, Mouse, Rat GRP78/HSPA5 ELISA Kit (Colorimetric) [NBP2-62145] - A typical standard curve for GRP78/HSPA5 ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.")
![ELISA: Human, Mouse, Rat GRP78/HSPA5 ELISA Kit (Colorimetric) [NBP2-62145] - To assess parallelism, HeLa, 3T3, and C6 lysates and serum were serially diluted into assay buffer and run in the assay. The GRP78 concentration in each sample was assigned using the standard curve. Assigned concentrations were plotted as a function of sa](http://images.novusbio.com/fullsize/Human-Mouse-Rat-GRP78-HSPA5-ELISA-Kit-Colorimetric-ELISA-NBP2-62145-img0002.jpg)
![ELISA: Human, Mouse, Rat GRP78/HSPA5 ELISA Kit (Colorimetric) [NBP2-62145] - ELISA versus Western blot analysis correlation comparison of GRP78/HSPA5 using C6 cell lysates. Lysates were serially diluted and either run in the ELISA or run on a 12% Tris-glycine gel, transferred to nitrocellulose membrane and probed for GRP78 with a](http://images.novusbio.com/fullsize/Human-Mouse-Rat-GRP78-HSPA5-ELISA-Kit-Colorimetric-ELISA-NBP2-62145-img0004.jpg)
![<b>Biological Strategies Validation. </b>ELISA: Human, Mouse, Rat GRP78/HSPA5 ELISA Kit (Colorimetric) [NBP2-62145] - HeLa cells were treated for 5 hours with 5 uM, 50 uM or 200 uM concentrations of cyclosporine A. Following the incubation, treated and untreated cells were lysed with Extraction Reagent #2 as described above. The lysates were then evaluated in the assay.](http://images.novusbio.com/fullsize/Human-Mouse-Rat-GRP78-HSPA5-ELISA-Kit-Colorimetric-ELISA-NBP2-62145-img0003.jpg)