| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-97069-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38175-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-99489-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90308-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-06585-50ul | |||
![Western Blot: GPD1 Antibody (HL1959) - Azide and BSA Free [NBP3-25488] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GPD1 antibody [HL1959] (NBP3-25488) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25488_rabbit-gpd1-mab-hl1959-azide-and-bsa-free-2120249393521.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: GPD1 Antibody (HL1959) - Azide and BSA Free [NBP3-25488] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GPD1 antibody [HL1959] (NBP3-25488) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: GPD1 Antibody (HL1959) - Azide and BSA Free [NBP3-25488] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GPD1 antibody [HL1959] (NBP3-25488) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25488_rabbit-gpd1-mab-hl1959-azide-and-bsa-free-2120249362727.jpg)
![Western Blot: GPD1 Antibody (HL1959) - Azide and BSA Free [NBP3-25488] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GPD1 antibody [HL1959] (NBP3-25488) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25488_rabbit-gpd1-mab-hl1959-azide-and-bsa-free-2120249383174.jpg)
![Immunohistochemistry-Paraffin: GPD1 Antibody (HL1959) - Azide and BSA Free [NBP3-25488] - GPD1 antibody [HL1959] detects GPD1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. GPD1 stained by GPD1 antibody [HL1959] (NBP3-25488) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25488_rabbit-gpd1-mab-hl1959-azide-and-bsa-free-212024939153.jpg)
![GPD1 antibody [HL1959] detects GPD1 protein at cell membrane and cytoplasm by immunofluorescent analysis.Sample: Caco-2 cells were fixed in ice-cold MeOH for 5 min.Green: GPD1 stained by GPD1 antibody [HL1959] (NBP3-25488) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25488_rabbit-gpd1-mab-hl1959-azide-and-bsa-free-immunocytochemistry-immunofluorescence-141120251841527.jpg)
![GPD1 antibody [HL1959] detects GPD1 protein by immunohistochemical analysis.Sample: Paraffin-embedded human tissues.GPD1 stained by GPD1 antibody [HL1959] (NBP3-25488) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25488_rabbit-gpd1-mab-hl1959-azide-and-bsa-free-immunohistochemistry-paraffin-1411202518404323.jpg)
![Western Blot: Glycerol 3 Phosphate Dehydrogenase Antibody [NBP2-16688] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/Glycerol-3-Phosphate-Dehydrogenase-Antibody-Western-Blot-NBP2-16688-img0008.jpg "Western Blot: Glycerol 3 Phosphate Dehydrogenase Antibody [NBP2-16688] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE diluted at 1:1000")
![Immunocytochemistry/Immunofluorescence: Glycerol 3 Phosphate Dehydrogenase Antibody [NBP2-16688] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GPD1 protein stained by GPD1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Glycerol-3-Phosphate-Dehydrogenase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16688-img0011.jpg)
![Immunohistochemistry-Paraffin: Glycerol 3 Phosphate Dehydrogenase Antibody [NBP2-16688] - Paraffin-embedded D54MG xenograft. GPD1 antibody dilution: 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min](http://images.novusbio.com/fullsize/Glycerol-3-Phosphate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP2-16688-img0010.jpg)
![Immunohistochemistry-Paraffin: Glycerol 3 Phosphate Dehydrogenase Antibody [NBP2-16688] - RT2 xenograft, using GPD1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Glycerol-3-Phosphate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP2-16688-img0006.jpg)
![Immunohistochemistry-Paraffin: Glycerol 3 Phosphate Dehydrogenase Antibody [NBP2-16688] - Paraffin-embedded Mahlarvu xenograft. GPD1 antibody dilution: 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min](http://images.novusbio.com/fullsize/Glycerol-3-Phosphate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP2-16688-img0009.jpg)
![Western Blot: Glycerol 3 Phosphate Dehydrogenase Antibody (3C10-1C4) [H00002819-M01] - Analysis of GPD1 expression in transfected 293T cell line by GPD1 monoclonal antibody (M01), clone 3C10-1C4.Lane 1: GPD1 transfected lysate (Predicted MW: 37.6 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Glycerol-3-Phosphate-Dehydrogenase-Antibody-3C10-1C4-Western-Blot-H00002819-M01-img0004.jpg "Western Blot: Glycerol 3 Phosphate Dehydrogenase Antibody (3C10-1C4) [H00002819-M01] - Analysis of GPD1 expression in transfected 293T cell line by GPD1 monoclonal antibody (M01), clone 3C10-1C4.Lane 1: GPD1 transfected lysate (Predicted MW: 37.6 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: Glycerol 3 Phosphate Dehydrogenase Antibody (3C10-1C4) [H00002819-M01] - GPD1 monoclonal antibody (M01), clone 3C10-1C4. Analysis of GPD1 expression in human kidney.](http://images.novusbio.com/fullsize/Glycerol-3-Phosphate-Dehydrogenase-Antibody-3C10-1C4-Western-Blot-H00002819-M01-img0003.jpg)
![Sandwich ELISA: Glycerol 3 Phosphate Dehydrogenase Antibody (3C10-1C4) [H00002819-M01] - Detection limit for recombinant GST tagged GPD1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Glycerol-3-Phosphate-Dehydrogenase-Antibody-3C10-1C4-Sandwich-ELISA-H00002819-M01-img0002.jpg)
![Western Blot: Glycerol 3 Phosphate Dehydrogenase Antibody [NBP2-97069] - Anti-Glycerol 3 Phosphate Dehydrogenase rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 35 kDa](http://images.novusbio.com/fullsize/Glycerol-3-Phosphate-Dehydrogenase-Antibody-Western-Blot-NBP2-97069-img0004.jpg "Western Blot: Glycerol 3 Phosphate Dehydrogenase Antibody [NBP2-97069] - Anti-Glycerol 3 Phosphate Dehydrogenase rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 35 kDa")
![Immunoprecipitation: Glycerol 3 Phosphate Dehydrogenase Antibody [NBP2-97069] - Glycerol 3 Phosphate Dehydrogenase was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. 0.5 ul anti-Glycerol 3 Phosphate Dehydrogenase rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Glycerol 3 Phosphate Dehydrogenase rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 38 kDa](http://images.novusbio.com/fullsize/Glycerol-3-Phosphate-Dehydrogenase-Antibody-Immunoprecipitation-NBP2-97069-img0003.jpg)
![Western Blot: Glycerol 3 Phosphate Dehydrogenase Antibody [NBP3-38175] - Western blot analysis of various lysates using Glycerol 3 Phosphate Dehydrogenase Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-38175_rabbit-glycerol-3-phosphate-dehydrogenase-pab-912202415171181.jpg "Western Blot: Glycerol 3 Phosphate Dehydrogenase Antibody [NBP3-38175] - Western blot analysis of various lysates using Glycerol 3 Phosphate Dehydrogenase Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.")
![Western Blot: Glycerol 3 Phosphate Dehydrogenase Antibody [NBP1-55332] - Titration: 0.2-1 ug/ml, Positive Control: Transfected 293T.](http://images.novusbio.com/fullsize/Glycerol-3-Phosphate-Dehydrogenase-Antibody-Western-Blot-NBP1-55332-img0003.jpg "Western Blot: Glycerol 3 Phosphate Dehydrogenase Antibody [NBP1-55332] - Titration: 0.2-1 ug/ml, Positive Control: Transfected 293T.")
![Western Blot: Glycerol 3 Phosphate Dehydrogenase Overexpression Lysate (Adult Normal) [NBL1-11224] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Glycerol 3 Phosphate Dehydrogenase.](http://images.novusbio.com/fullsize/Glycerol-3-Phosphate-Dehydrogenase-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11224-img0002.jpg "Western Blot: Glycerol 3 Phosphate Dehydrogenase Overexpression Lysate (Adult Normal) [NBL1-11224] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Glycerol 3 Phosphate Dehydrogenase.")
![SDS-Page: Recombinant Human Glycerol 3 Phosphate Dehydrogenase Protein [NBP1-40405] - GPD1, 37.5 kDa (349aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE](http://images.novusbio.com/fullsize/Glycerol-3-Phosphate-Dehydrogenase-Protein-SDS-Page-NBP1-40405-img0001.jpg "SDS-Page: Recombinant Human Glycerol 3 Phosphate Dehydrogenase Protein [NBP1-40405] - GPD1, 37.5 kDa (349aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE")
![Immunohistochemistry-Paraffin: Glycerol 3 Phosphate Dehydrogenase Antibody [NBP2-99489] - Immunochemical staining of human Glycerol 3 Phosphate Dehydrogenase in human kidney with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Glycerol-3-Phosphate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP2-99489-img0001.jpg "Immunohistochemistry-Paraffin: Glycerol 3 Phosphate Dehydrogenase Antibody [NBP2-99489] - Immunochemical staining of human Glycerol 3 Phosphate Dehydrogenase in human kidney with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.")