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NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">
NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="FGF-20 was detected in immersion fixed SW480 human colorectal adenocarcinoma cell line using Rat Anti-Human FGF-20 Monoclonal Antibody (Catalog # MAB2547) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
2547-FG) stimulates proliferation in the the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF-20 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF-20 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2547). The ND50 is typically 0.05-0.35 µg/mL in the presence of heparin (1 µg/mL)." class="big_lightbox">
2547-FG) stimulates proliferation in the the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF-20 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF-20 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2547). The ND50 is typically 0.05-0.35 µg/mL in the presence of heparin (1 µg/mL)." alt="Recombinant Human FGF-20 (Catalog # 2547-FG) stimulates proliferation in the the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF-20 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF-20 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2547). The ND50 is typically 0.05-0.35 µg/mL in the presence of heparin (1 µg/mL)." class="big_thumb" />