| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-02960-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34643-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89187-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34641-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20928 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-48005-0.1mg | |||
![Immunohistochemistry: ErbB2/Her2 Antibody (PD00-53) [NBP3-32422] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-ErbB2/Her2 antibody (NBP3-32422) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32422) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32422_rabbit-erbb2-her2-mab-pd00-53-298202414262699.jpg "Immunohistochemistry: ErbB2/Her2 Antibody (PD00-53) [NBP3-32422] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-ErbB2/Her2 antibody (NBP3-32422) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32422) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 Antibody (PD00-53) [NBP3-32422] - Immunocytochemistry analysis of MCF-7 cells labeling ErbB2/Her2 with Rabbit anti-ErbB2/Her2 antibody (NBP3-32422) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-ErbB2/Her2 antibody (NBP3-32422) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32422_rabbit-erbb2-her2-mab-pd00-53-29202410234277.jpg)
![Flow Cytometry: ErbB2/Her2 Antibody (PD00-53) [NBP3-32422] - Flow cytometric analysis of SK-Br-3 cells labeling ErbB2/Her2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32422, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32422_rabbit-erbb2-her2-mab-pd00-53-29202416453290.jpg)
![Western Blot: ErbB2/Her2 Antibody [NB100-1710] - Detection of Human ErbB2 on HeLa whole cell lysate using NB100-1710. ErbB2 was also immunoprecipitated using rabbit anti-ErbB2 antibodies NB100-1711 and NB100-1712.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-Western-Blot-NB100-1710-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: ErbB2/Her2 Antibody [NB100-1710] - Detection of Human ErbB2 on HeLa whole cell lysate using NB100-1710. ErbB2 was also immunoprecipitated using rabbit anti-ErbB2 antibodies NB100-1711 and NB100-1712.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NB100-1710] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ErbB2 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-Immunohistochemistry-NB100-1710-img0003.jpg)
![Western Blot: ErbB2/Her2 Antibody [NBP3-02960] - Analysis of extracts of Mouse lung, using HER2 / ErbB2 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-Western-Blot-NBP3-02960-img0006.jpg "Western Blot: ErbB2/Her2 Antibody [NBP3-02960] - Analysis of extracts of Mouse lung, using HER2 / ErbB2 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP3-02960] - Rat testis using [KO Validated] HER2 / ErbB2 Rabbit pAb at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-Immunohistochemistry-Paraffin-NBP3-02960-img0007.jpg)
![Western Blot: ErbB2/Her2 Antibody [NBP3-02960] - Analysis of extracts of SK-BR-3 cells, using HER2 / ErbB2 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-Western-Blot-NBP3-02960-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/718) [NBP2-32864] - Immunofluorescent staining of PFA-fixed MCF-7 cells with ErbB2/Her2 Monospecific Mouse Monoclonal Antibody (HRB2/718) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-718-Immunocytochemistry-Immunofluorescence-NBP2-32864-img0005.jpg "Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/718) [NBP2-32864] - Immunofluorescent staining of PFA-fixed MCF-7 cells with ErbB2/Her2 Monospecific Mouse Monoclonal Antibody (HRB2/718) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).")
![Flow Cytometry: ErbB2/Her2 Antibody (HRB2/718) [NBP2-32864] - Flow Cytometric Analysis of human MCF-7 cells. ErbB2/Her2 Antibody (HRB2/718) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-718-Flow-Cytometry-NBP2-32864-img0003.jpg)
![Protein Array: ErbB2/Her2 Antibody (HRB2/718) [NBP2-32864] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ErbB2/Her2 Antibody (HRB2/718) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-718-Protein-Array-NBP2-32864-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (511) [NBP2-89187] - Staining of Human ErbB2 in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human ErbB2 monoclonal antibody (1:100) at 4c overnight. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-511-Immunocytochemistry-Immunofluorescence-NBP2-89187-img0001.jpg "Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (511) [NBP2-89187] - Staining of Human ErbB2 in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human ErbB2 monoclonal antibody (1:100) at 4c overnight. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.")
![Flow Cytometry: ErbB2/Her2 Antibody (511) [NBP2-89187] - Analysis of anti-ErbB2 on MCF-7 cells. MCF-7 cells were detached using 1X trypsin, washed, then stained with purified rabbit anti-human ErbB2. Second step staining with FITC conjugated goat anti-rabbit IgG (H+L) polyclonal antibody.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-511-Flow-Cytometry-NBP2-89187-img0003.jpg)
![Flow Cytometry: ErbB2/Her2 Antibody (511) [NBP2-89187] - Analysis of anti-ErbB2 on SK-BR3 cells. SK-BR3 cells were detached using 1X trypsin, washed, then stained with Purified Rabbit anti-Human ErbB2. Second step staining with FITC conjugated Goat anti-Rabbit IgG polyclonal antibody (BD Pharmingen (R).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-511-Flow-Cytometry-NBP2-89187-img0002.jpg)
![Flow Cytometry: ErbB2/Her2 Antibody (HRB2/258) [NBP2-32862] - TUBO murine mammary cancer cells expression of rat ErbB-2 receptor. Grey histogram is unstained cells, while red is cells incubated with 1:500 dilution of anti-rat-ErbB-2-AF488 in the dark for 30 minutes on ice. Image from verified customer review. Image using the AlexaFluor 488 format of this antibody.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-258-Flow-Cytometry-NBP2-32862-img0002.jpg "Flow Cytometry: ErbB2/Her2 Antibody (HRB2/258) [NBP2-32862] - TUBO murine mammary cancer cells expression of rat ErbB-2 receptor. Grey histogram is unstained cells, while red is cells incubated with 1:500 dilution of anti-rat-ErbB-2-AF488 in the dark for 30 minutes on ice. Image from verified customer review. Image using the AlexaFluor 488 format of this antibody.")
![Western Blot: ErbB2/Her2 [p Tyr1139] Antibody (JE60-90) [NBP3-32768] - Western blot analysis of ErbB2/Her2 on different lysates with Rabbit anti-ErbB2/Her2 antibody (NBP3-32768) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: A431 treated with 100ng/mL EGF for 10 minutes cell lysate Lane 3: A431 treated with 100ng/mL EGF for 10 minutes cell lysate, then the membrane treated with pp for 1 hour Lane 4: SK-Br-3 cell lysate Lane 5: SK-Br-3 starved for 4 hours add 200ng/mL EGF for 15 minutes cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 138 kDa Observed band size: 180 kDa Exposure time: 10 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32768) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32768_rabbit-erbb2-her2-p-tyr1139-mab-je60-90-288202412442922.jpg "Western Blot: ErbB2/Her2 [p Tyr1139] Antibody (JE60-90) [NBP3-32768] - Western blot analysis of ErbB2/Her2 on different lysates with Rabbit anti-ErbB2/Her2 antibody (NBP3-32768) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: A431 treated with 100ng/mL EGF for 10 minutes cell lysate Lane 3: A431 treated with 100ng/mL EGF for 10 minutes cell lysate, then the membrane treated with pp for 1 hour Lane 4: SK-Br-3 cell lysate Lane 5: SK-Br-3 starved for 4 hours add 200ng/mL EGF for 15 minutes cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 138 kDa Observed band size: 180 kDa Exposure time: 10 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32768) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 [p Tyr1139] Antibody (JE60-90) [NBP3-32768] - Immunocytochemistry analysis of A431 cells treated with or without 100ng/mL EGF for 10 minutes labeling ErbB2/Her2 with Rabbit anti-ErbB2/Her2 antibody (NBP3-32768) at 1/5,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ErbB2/Her2 antibody (NBP3-32768) at 1/5,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32768_rabbit-erbb2-her2-p-tyr1139-mab-je60-90-29202410484847.jpg)
![Flow Cytometry: ErbB2/Her2 [p Tyr1139] Antibody (JE60-90) [NBP3-32768] - Flow cytometric analysis of A431 cells treated with or without 100ng/mL EGF for 10 minutes labeling ErbB2/Her2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32768, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32768_rabbit-erbb2-her2-p-tyr1139-mab-je60-90-2920241637217.jpg)
![Western Blot: ErbB2/Her2 [p Tyr1222, p Tyr1221] Antibody [NBP3-13232] - Untreated (-) and treated (+) MCF-7 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ErbB2/Her2 (phospho Tyr1221/Tyr1222) antibody (NBP3-13232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ErbB2-Her2-[p-Tyr1222--p-Tyr1221]-Antibody-Western-Blot-NBP3-13232-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: ErbB2/Her2 [p Tyr1222, p Tyr1221] Antibody [NBP3-13232] - Untreated (-) and treated (+) MCF-7 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ErbB2/Her2 (phospho Tyr1221/Tyr1222) antibody (NBP3-13232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/273) [NBP2-44984] - ICC/IF staining of MCF-7 cells with followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Red Dot (Red).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-273-Immunofluorescence-NBP2-44984-img0001.jpg "Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/273) [NBP2-44984] - ICC/IF staining of MCF-7 cells with followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Red Dot (Red).")