| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89184-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52639-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-24037 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55546-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-24341 | |||
![Western Blot: ErbB2/Her2 Antibody (PD00-99) [NBP3-32423] - Western blot analysis of ErbB2/Her2 on SK-Br-3 cell lysates with Rabbit anti-ErbB2/Her2 antibody (NBP3-32423) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 138 kDa Observed band size: 180 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32423) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32423_rabbit-erbb2-her2-mab-pd00-99-278202415242676.jpg "Western Blot: ErbB2/Her2 Antibody (PD00-99) [NBP3-32423] - Western blot analysis of ErbB2/Her2 on SK-Br-3 cell lysates with Rabbit anti-ErbB2/Her2 antibody (NBP3-32423) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 138 kDa Observed band size: 180 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32423) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ErbB2/Her2 Antibody (PD00-99) [NBP3-32423] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-ErbB2/Her2 antibody (NBP3-32423) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32423) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32423_rabbit-erbb2-her2-mab-pd00-99-298202414333621.jpg)
![Western Blot: ErbB2/Her2 [p Tyr1139] Antibody [NBP3-12918] - Untreated (-) and treated (+) A431 whole cell extracts (5 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ErbB2/Her2 (phospho Tyr1139) antibody (NBP3-12918) diluted at 1:10000.](http://images.novusbio.com/fullsize/ErbB2-Her2-[p-Tyr1139]-Antibody-Western-Blot-NBP3-12918-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: ErbB2/Her2 [p Tyr1139] Antibody [NBP3-12918] - Untreated (-) and treated (+) A431 whole cell extracts (5 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ErbB2/Her2 (phospho Tyr1139) antibody (NBP3-12918) diluted at 1:10000.")
![<b>Biological Strategies Validation. </b>Western Blot: ErbB2/Her2 [p Tyr1139] Antibody [NBP3-12918] - ErbB2 (phospho Tyr1139) antibody detects ErbB2 (phospho Tyr1139) protein by western blot analysis. Un-treated (-) and treated (+, 200ng/ml Trichostatin A treatment for 15min) A431 whole cell extracts (5 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ErbB2 (phospho Tyr1139) antibody (NBP3-12918) at a dilution of 1:10000.](http://images.novusbio.com/fullsize/ErbB2-Her2-[p-Tyr1139]-Antibody-Western-Blot-NBP3-12918-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (002) [NBP2-89184] - Staining of Human ErbB2 in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human ErbB2 monoclonal antibody (1:100) at 4c overnight. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody ( green ) and counterstained with DAPI ( blue ). Positive staining was localized to plasma membrane.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-002-Immunocytochemistry-Immunofluorescence-NBP2-89184-img0001.jpg "Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (002) [NBP2-89184] - Staining of Human ErbB2 in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human ErbB2 monoclonal antibody (1:100) at 4c overnight. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody ( green ) and counterstained with DAPI ( blue ). Positive staining was localized to plasma membrane.")
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (002) [NBP2-89184] - Staining of human ERBB2 in human breast carcinoma with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-002-Immunohistochemistry-Paraffin-NBP2-89184-img0002.jpg)
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (4D5-8 (trastuzumab)) - Chimeric [NBP2-52639] - Immunohistochemical Anti-erbB-2 (Her-2/neu) staining of paraffin embedded human breast tissue using the rabbit-chimeric version of 4D5-8 (trastuzumab) (NBP2-52639). Primary antibody incubation with NBP2-52639 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Weak membrane staining exclusively of ductal epithelial cells may be observed. Recommended concentration, 4-6 ug/ml.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-4D5-8-trastuzumab-Chimeric-Immunohistochemistry-Paraffin-NBP2-52639-img0001.jpg "Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (4D5-8 (trastuzumab)) - Chimeric [NBP2-52639] - Immunohistochemical Anti-erbB-2 (Her-2/neu) staining of paraffin embedded human breast tissue using the rabbit-chimeric version of 4D5-8 (trastuzumab) (NBP2-52639). Primary antibody incubation with NBP2-52639 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Weak membrane staining exclusively of ductal epithelial cells may be observed. Recommended concentration, 4-6 ug/ml.")
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (ERBB2/7158R) [NBP3-23595] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with ErbB2/Her2 Antibody (ERBB2/7158R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/nbp3-23595_rabbit-erbb2-her2-mab-erbb2-7158r-2412025872385.jpg "Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (ERBB2/7158R) [NBP3-23595] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with ErbB2/Her2 Antibody (ERBB2/7158R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.")
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (ERBB2/7158R) [NBP3-23595] - IHC analysis of formalin-fixed, paraffin-embedded human breast stroma. Negative tissue control using ErbB2/Her2 Antibody (ERBB2/7158R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/nbp3-23595_rabbit-erbb2-her2-mab-erbb2-7158r-2412025863690.jpg)
![Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody [NBP2-55546] - Staining of human cell line A-431 shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55546-img0001.jpg "Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody [NBP2-55546] - Staining of human cell line A-431 shows localization to plasma membrane. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (rERBB2/6637) [NBP3-23581] - IHC analysis of formalin-fixed, paraffin-embedded human breast carcinoma. Stained using ErbB2/Her2 Antibody (rERBB2/6637) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/nbp3-23581_mouse-erbb2-her2-mab-rerbb2-6637-2412025872320.jpg "Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (rERBB2/6637) [NBP3-23581] - IHC analysis of formalin-fixed, paraffin-embedded human breast carcinoma. Stained using ErbB2/Her2 Antibody (rERBB2/6637) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.")
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (rERBB2/6637) [NBP3-23581] - IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using ErbB2/Her2 Antibody (rERBB2/6637) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/nbp3-23581_mouse-erbb2-her2-mab-rerbb2-6637-2412025863666.jpg)
![Western Blot: ErbB2/Her2 [p Tyr1196] Antibody [NBP3-13268] - Untreated (-) and treated (+) MCF-7 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ErbB2/Her2 (phospho Tyr1196) antibody (NBP3-13268) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ErbB2-Her2-[p-Tyr1196]-Antibody-Western-Blot-NBP3-13268-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: ErbB2/Her2 [p Tyr1196] Antibody [NBP3-13268] - Untreated (-) and treated (+) MCF-7 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ErbB2/Her2 (phospho Tyr1196) antibody (NBP3-13268) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: ErbB2/HER2 Overexpression Lysate (Adult Normal) [NBL1-10314] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ErbB2/HER2.](http://images.novusbio.com/fullsize/ErbB2-HER2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10314-img0002.jpg "Western Blot: ErbB2/HER2 Overexpression Lysate (Adult Normal) [NBL1-10314] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ErbB2/HER2.")
![Western Blot: ErbB2/Her2 [p Tyr1139] Antibody (7N1S0) [NBP3-16960] - Western blot analysis of extracts of A-431 cells, using ErbB2/Her2 (NBP3-16960) at 1:500 dilution.A-431 cells were treated by EGF (100 ng/ml) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/ErbB2-Her2-p-Tyr1139-Antibody-7N1S0-Western-Blot-NBP3-16960-img0001.jpg "Western Blot: ErbB2/Her2 [p Tyr1139] Antibody (7N1S0) [NBP3-16960] - Western blot analysis of extracts of A-431 cells, using ErbB2/Her2 (NBP3-16960) at 1:500 dilution.A-431 cells were treated by EGF (100 ng/ml) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Flow Cytometry: ErbB2/Her2 Antibody (24D2) [NBP3-09136] - Separation of MCF-7 cells stained using anti-human CD340 (24D2) purified antibody (concentration in sample 5,0 ug/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-24D2-Flow-Cytometry-NBP3-09136-img0001.jpg "Flow Cytometry: ErbB2/Her2 Antibody (24D2) [NBP3-09136] - Separation of MCF-7 cells stained using anti-human CD340 (24D2) purified antibody (concentration in sample 5,0 ug/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).")