| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98336-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-05342-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98433-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-23977 | |||
![Western Blot: E-Cadherin Antibody [NBP2-98336] - Anti-E-Cadherin rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: MDA-MB-468 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 97 kDa. Observed band size: 150 kDa](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-Western-Blot-NBP2-98336-img0004.jpg "Western Blot: E-Cadherin Antibody [NBP2-98336] - Anti-E-Cadherin rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: MDA-MB-468 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 97 kDa. Observed band size: 150 kDa")
![Immunohistochemistry-Paraffin: E-Cadherin Antibody [NBP2-98336] - Immunochemical staining of mouse E-Cadherin in mouse stomach with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-Immunohistochemistry-Paraffin-NBP2-98336-img0003.jpg)
![Immunoprecipitation: E-Cadherin Antibody [NBP2-98336] - Mouse E-Cadherin was immunoprecipitated using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate. 2 ul anti-Mouse E-Cadherin rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse E-Cadherin rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 130 kDa. Observed band size: 130 kDa](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-Immunoprecipitation-NBP2-98336-img0001.jpg)
![Western Blot: E-Cadherin [p Ser838, p Ser840] Antibody (S03-3B3) [NBP3-19826] - Western blot detection of E-Cadherin (phospho-Ser838/Ser840) in HepG2, U2OS, U251, Lncap using NBP3-19826 (1:1000 diluted)](http://images.novusbio.com/fullsize/E-Cadherin-p-Ser838-p-Ser840-Antibody-S03-3B3-Western-Blot-NBP3-19826-img0002.jpg "Western Blot: E-Cadherin [p Ser838, p Ser840] Antibody (S03-3B3) [NBP3-19826] - Western blot detection of E-Cadherin (phospho-Ser838/Ser840) in HepG2, U2OS, U251, Lncap using NBP3-19826 (1:1000 diluted)")
![Immunohistochemistry-Paraffin: E-Cadherin [p Ser838, p Ser840] Antibody (S03-3B3) [NBP3-19826] - Immunohistochemistry of E-Cadherin (phospho-Ser838/Ser840) in paraffin-embedded Human breast cancer tissue using NBP3-19826 at dilution 1/50](http://images.novusbio.com/fullsize/E-Cadherin-p-Ser838-p-Ser840-Antibody-S03-3B3-Immunohistochemistry-Paraffin-NBP3-19826-img0001.jpg)
![Western Blot: pan Cadherin Antibody (2M6G8) [NBP3-05342] - analysis of extracts of various cell lines, using E-Cadherin antibody at 1:700 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/antibody/nbp3-05342_mouse-polyclonal-pan-cadherin-antibody-amc0108-western-blot-199202311401..jpg "Western Blot: pan Cadherin Antibody (2M6G8) [NBP3-05342] - analysis of extracts of various cell lines, using E-Cadherin antibody at 1:700 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")
![ICC/IF-pan Cadherin Antibody (2M6G8) [NBP3-05342] - Analysis of mouse kidney cells using E-Cadherin Mouse mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/antibody/nbp3-05342_mouse-polyclonal-pan-cadherin-antibody-amc0108-immunocytochemistry-immunofluorescence-1992023114216..jpg)
![Western Blot: pan Cadherin Antibody (6N5P8) [NBP3-16509] - Western blot analysis of extracts of various cell lines, using pan Cadherin Rabbit mAb (NBP3-16509) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/pan-Cadherin-Antibody-6N5P8-Western-Blot-NBP3-16509-img0002.jpg "Western Blot: pan Cadherin Antibody (6N5P8) [NBP3-16509] - Western blot analysis of extracts of various cell lines, using pan Cadherin Rabbit mAb (NBP3-16509) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: pan Cadherin Antibody (6N5P8) [NBP3-16509] - Immunohistochemistry of paraffin-embedded human thyroid cancer using pan Cadherin Rabbit mAb (NBP3-16509) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/pan-Cadherin-Antibody-6N5P8-Immunohistochemistry-Paraffin-NBP3-16509-img0001.jpg)
![Western Blot: E-Cadherin Antibody [NBP2-98433] - Lane A: MCF7 Whole Cell LysateLane B: Caco-2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDaObserved band size:50 kDa](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-Western-Blot-NBP2-98433-img0002.jpg "Western Blot: E-Cadherin Antibody [NBP2-98433] - Lane A: MCF7 Whole Cell LysateLane B: Caco-2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDaObserved band size:50 kDa")
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody [NBP2-98433] - Immunofluorescence staining of E-Cadherin in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-rat E-Cadherin polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG Secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cell membrane and cytoplasm.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98433-img0001.jpg)
![Western Blot: E-Cadherin Antibody (A0-G11-2-R) [NBP3-32289] - Western blot analysis of E-Cadherin on different lysates with Mouse anti-E-Cadherin antibody (NBP3-32289) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: SW480 cell lysate Lane 3: MCF7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 97.5 kDa Observed band size: 130 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32289) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32289_mouse-e-cadherin-mab-a0-g11-2-r-27820241628529.jpg "Western Blot: E-Cadherin Antibody (A0-G11-2-R) [NBP3-32289] - Western blot analysis of E-Cadherin on different lysates with Mouse anti-E-Cadherin antibody (NBP3-32289) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: SW480 cell lysate Lane 3: MCF7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 97.5 kDa Observed band size: 130 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32289) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: E-Cadherin Antibody (A0-G11-2-R) [NBP3-32289] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-E-Cadherin antibody (NBP3-32289) at 1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32289) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32289_mouse-e-cadherin-mab-a0-g11-2-r-298202413585533.jpg)
![Western Blot: pan Cadherin Antibody (GT2610) [NBP3-13558] - Non-transfected (-) and transfected (+) MCF-7 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Pan Cadherin antibody [GT2610] (NBP3-13558) diluted at 1:500.](http://images.novusbio.com/fullsize/pan-Cadherin-Antibody-GT2610-Western-Blot-NBP3-13558-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: pan Cadherin Antibody (GT2610) [NBP3-13558] - Non-transfected (-) and transfected (+) MCF-7 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Pan Cadherin antibody [GT2610] (NBP3-13558) diluted at 1:500.")
![<b>Genetic Strategies Validation. </b>Western Blot: pan Cadherin Antibody (GT2610) [NBP3-13558] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Pan Cadherin antibody [GT2610] (NBP3-13558) diluted at 1:1000.](http://images.novusbio.com/fullsize/pan-Cadherin-Antibody-GT2610-Western-Blot-NBP3-13558-img0001.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (rCDH1/6769) [NBP3-23653] - IHC analysis of formalin-fixed, paraffin-embedded human stomach. Stained using E-Cadherin Antibody (rCDH1/6769) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/nbp3-23653_mouse-e-cadherin-mab-rcdh1-6769-2412025863614.jpg "Immunohistochemistry-Paraffin: E-Cadherin Antibody (rCDH1/6769) [NBP3-23653] - IHC analysis of formalin-fixed, paraffin-embedded human stomach. Stained using E-Cadherin Antibody (rCDH1/6769) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.")
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (rCDH1/6769) [NBP3-23653] - Formalin-fixed, paraffin-embedded human colon stained with E-Cadherin Antibody (rCDH1/6769). Inset: PBS instead of primary antibody; secondary only negative control.](http://images.novusbio.com/fullsize/nbp3-23653_mouse-e-cadherin-mab-rcdh1-6769-2412025872375.jpg)
![Western Blot: E-Cadherin Overexpression Lysate (Adult Normal) [NBL1-09024] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for E-Cadherin.](http://images.novusbio.com/fullsize/E-Cadherin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09024-img0002.jpg "Western Blot: E-Cadherin Overexpression Lysate (Adult Normal) [NBL1-09024] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for E-Cadherin.")
![ELISA: Mouse E-Cadherin ELISA Kit (Colorimetric) [NBP2-67947] - Samples were spiked with high concentrations of Mouse E-Cadherin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-67947_mouse-e-cadherin-elisa-kit-colorimetric-132202416133646.jpg "ELISA: Mouse E-Cadherin ELISA Kit (Colorimetric) [NBP2-67947] - Samples were spiked with high concentrations of Mouse E-Cadherin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse E-Cadherin ELISA Kit (Colorimetric) [NBP2-67947] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-67947_mouse-e-cadherin-elisa-kit-colorimetric-4320249163356.jpg)
![ELISA: Mouse E-Cadherin ELISA Kit (Chemiluminescence) [NBP2-67946] - Samples were spiked with high concentrations of Mouse E-Cadherin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-67946_mouse-e-cadherin-elisa-kit-chemiluminescence-132202416254014.jpg "ELISA: Mouse E-Cadherin ELISA Kit (Chemiluminescence) [NBP2-67946] - Samples were spiked with high concentrations of Mouse E-Cadherin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse E-Cadherin ELISA Kit (Chemiluminescence) [NBP2-67946] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-67946_mouse-e-cadherin-elisa-kit-chemiluminescence-432024955049.jpg)
![Immunohistochemistry: E-Cadherin Antibody (1E1) [NBP3-15180] - IHC image of E-Cadherin antibody diluted at 1:100 and staining in paraffin-embedded human breast cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-1E1-Immunohistochemistry-NBP3-15180-img0001.jpg "Immunohistochemistry: E-Cadherin Antibody (1E1) [NBP3-15180] - IHC image of E-Cadherin antibody diluted at 1:100 and staining in paraffin-embedded human breast cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")