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![Western Blot: CYP27A1 Antibody (HL1820) - Azide and BSA Free [NBP3-25429] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP27A1 antibody [HL1820] (NBP3-25429) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25429_rabbit-cyp27a1-mab-hl1820-azide-and-bsa-free-2120249374976.jpg "Western Blot: CYP27A1 Antibody (HL1820) - Azide and BSA Free [NBP3-25429] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP27A1 antibody [HL1820] (NBP3-25429) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: CYP27A1 Antibody (HL1820) - Azide and BSA Free [NBP3-25429] - CYP27A1 antibody [HL1820] detects CYP27A1 protein at mitochondria by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: CYP27A1 stained by CYP27A1 antibody [HL1820] (NBP3-25429) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25429_rabbit-cyp27a1-mab-hl1820-azide-and-bsa-free-2120249374969.jpg)
![Immunohistochemistry-Paraffin: CYP27A1 Antibody (HL1820) - Azide and BSA Free [NBP3-25429] - CYP27A1 antibody [HL1820] detects CYP27A1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. CYP27A1 stained by CYP27A1 antibody [HL1820] (NBP3-25429) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25429_rabbit-cyp27a1-mab-hl1820-azide-and-bsa-free-2120249342711.jpg)
![Immunohistochemistry-Paraffin: CYP27A1 Antibody (HL1820) - Azide and BSA Free [NBP3-25429] - CYP27A1 antibody [HL1820] detects CYP27A1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. CYP27A1 stained by CYP27A1 antibody [HL1820] (NBP3-25429) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25429_rabbit-cyp27a1-mab-hl1820-azide-and-bsa-free-2120249432835.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: CYP27A1 Antibody (HL1820) - Azide and BSA Free [NBP3-25429] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP27A1 antibody [HL1820] (NBP3-25429) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25429_rabbit-cyp27a1-mab-hl1820-azide-and-bsa-free-212024940872.jpg)
![Western Blot: CYP27A1 Antibody (HL1820) - Azide and BSA Free [NBP3-25429] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP27A1 antibody [HL1820] (NBP3-25429) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25429_rabbit-cyp27a1-mab-hl1820-azide-and-bsa-free-2120249432852.jpg)
![Immunohistochemistry-Paraffin: CYP27A1 Antibody (HL1819) - Azide and BSA Free [NBP3-25428] - CYP27A1 antibody [HL1819] detects CYP27A1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. CYP27A1 stained by CYP27A1 antibody [HL1819] (NBP3-25428) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25428_rabbit-cyp27a1-mab-hl1819-azide-and-bsa-free-2120249393514.jpg "Immunohistochemistry-Paraffin: CYP27A1 Antibody (HL1819) - Azide and BSA Free [NBP3-25428] - CYP27A1 antibody [HL1819] detects CYP27A1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. CYP27A1 stained by CYP27A1 antibody [HL1819] (NBP3-25428) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: CYP27A1 Antibody (HL1819) - Azide and BSA Free [NBP3-25428] - CYP27A1 antibody [HL1819] detects CYP27A1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. CYP27A1 stained by CYP27A1 antibody [HL1819] (NBP3-25428) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25428_rabbit-cyp27a1-mab-hl1819-azide-and-bsa-free-2120249362746.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: CYP27A1 Antibody (HL1819) - Azide and BSA Free [NBP3-25428] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP27A1 antibody [HL1819] (NBP3-25428) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25428_rabbit-cyp27a1-mab-hl1819-azide-and-bsa-free-2120249415912.jpg)
![Western Blot: CYP27A1 Antibody (6L9D1) [NBP3-33416] - Western blot analysis of various lysates, using CYP27A1 Rabbit mAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-33416_rabbit-cyp27a1-mab-6l9d1-1012202411592847.jpg "Western Blot: CYP27A1 Antibody (6L9D1) [NBP3-33416] - Western blot analysis of various lysates, using CYP27A1 Rabbit mAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")