| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49547-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57251-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97398-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-04381-20ul | |||
![Western Blot: CTR9 Antibody [NB100-68205] - Detection of Human and Mouse CTR9 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CTR9 antibody NB100-68205 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CTR9-Antibody-Western-Blot-NB100-68205-img0012.jpg "<b>Biological Strategies Validation. </b>Western Blot: CTR9 Antibody [NB100-68205] - Detection of Human and Mouse CTR9 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CTR9 antibody NB100-68205 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: CTR9 Antibody [NB100-68205] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-CTR9 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/CTR9-Antibody-Immunohistochemistry-NB100-68205-img0010.jpg)
![Western Blot: CTR9 Antibody [NB100-68205] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. NB100-68205 used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.](http://images.novusbio.com/fullsize/CTR9-Antibody-Western-Blot-NB100-68205-img0002.jpg)
![Immunohistochemistry-Paraffin: CTR9 Antibody [NB100-68205] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CTR9 used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/CTR9-Antibody-Immunohistochemistry-Paraffin-NB100-68205-img0006.jpg)
![Immunohistochemistry-Paraffin: CTR9 Antibody [NB100-68205] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-CTR9 used at a dilution of 1:250.](http://images.novusbio.com/fullsize/CTR9-Antibody-Immunohistochemistry-Paraffin-NB100-68205-img0001.jpg)
![Immunohistochemistry: CTR9 Antibody [NB100-68205] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CTR9 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/CTR9-Antibody-Immunohistochemistry-NB100-68205-img0009.jpg)
![Immunoprecipitation: CTR9 Antibody [NB100-68205] - Detection of human CTR9 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CTR9 antibody NB100-68205 (lot NB100-68205-4) used for IP at 6 ug per reaction. CTR9 was also immunoprecipitated by a previous lot of this antibody (lot NB100-68205-3). For blotting immunoprecipitated CTR9, NB100-68205 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CTR9-Antibody-Immunoprecipitation-NB100-68205-img0013.jpg)
![Western Blot: CTR9 Antibody [NBP2-49547] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MGLane 4: Human plasmaLane 5: Human Liver tissueLane 6: Human Tonsil tissue](http://images.novusbio.com/fullsize/CTR9-Antibody-Western-Blot-NBP2-49547-img0003.jpg "Western Blot: CTR9 Antibody [NBP2-49547] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MGLane 4: Human plasmaLane 5: Human Liver tissueLane 6: Human Tonsil tissue")
![Immunocytochemistry/Immunofluorescence: CTR9 Antibody [NBP2-49547] - Staining of human cell line RT4 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/CTR9-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-49547-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CTR9 Antibody [NBP2-49547] - Staining in human urinary bladder and pancreas tissues using anti-CTR9 antibody. Corresponding CTR9 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CTR9-Antibody-Immunohistochemistry-Paraffin-NBP2-49547-img0007.jpg)
![Immunohistochemistry-Paraffin: CTR9 Antibody [NBP2-49547] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/CTR9-Antibody-Immunohistochemistry-Paraffin-NBP2-49547-img0005.jpg)
![Immunohistochemistry-Paraffin: CTR9 Antibody [NBP2-49547] - Staining of human urinary bladder shows high expression.](http://images.novusbio.com/fullsize/CTR9-Antibody-Immunohistochemistry-Paraffin-NBP2-49547-img0006.jpg)
![Western Blot: CTR9 Antibody [NBP2-16031] - A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 5 % SDS-PAGE CTR9 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/CTR9-Antibody-Western-Blot-NBP2-16031-img0007.jpg "Western Blot: CTR9 Antibody [NBP2-16031] - A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 5 % SDS-PAGE CTR9 antibody dilution: 1:1000")
![Immunohistochemistry-Paraffin: CTR9 Antibody [NBP2-16031] - Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/CTR9-Antibody-Immunohistochemistry-Paraffin-NBP2-16031-img0002.jpg)
![Western Blot: CTR9 Antibody [NBP2-16031] - Sample (30 ug of whole cell lysate) A: A431 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/CTR9-Antibody-Western-Blot-NBP2-16031-img0001.jpg)
![Western Blot: CTR9 Antibody [NBP3-18677] - Figure 2. Western blot analysis using the antibody directed against CTR9 (1) Western blot was performed on nuclear extracts and whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse CTR9 , diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 133 kDa) is indicated on the right.](http://images.novusbio.com/fullsize/CTR9-Antibody-Western-Blot-NBP3-18677-img0001.jpg "Western Blot: CTR9 Antibody [NBP3-18677] - Figure 2. Western blot analysis using the antibody directed against CTR9 (1) Western blot was performed on nuclear extracts and whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse CTR9 , diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 133 kDa) is indicated on the right.")
![ELISA: CTR9 Antibody [NBP3-18677] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse CTR9 . The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:4,600.](http://images.novusbio.com/fullsize/CTR9 Antibody-ELISA-NBP3-18677-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CTR9 Antibody [NBP2-57251] - Staining of human cell line HEK 293 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/CTR9-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57251-img0001.jpg "Immunocytochemistry/Immunofluorescence: CTR9 Antibody [NBP2-57251] - Staining of human cell line HEK 293 shows localization to nucleoplasm.")
![Western Blot: CTR9 Overexpression Lysate (Adult Normal) [NBL1-09582] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CTR9.](http://images.novusbio.com/fullsize/CTR9-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09582-img0002.jpg "Western Blot: CTR9 Overexpression Lysate (Adult Normal) [NBL1-09582] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CTR9.")
![Immunocytochemistry/Immunofluorescence: CTR9 Antibody [NBP2-97398] - Immunofluorescence staining of CTR9 in RT4 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97398 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp2-97398_rabbit-polyclonal-ctr9-antibody-235202514492344.png "Immunocytochemistry/Immunofluorescence: CTR9 Antibody [NBP2-97398] - Immunofluorescence staining of CTR9 in RT4 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97398 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.")
![Western Blot: CTR9 Antibody [NBP3-04381] - Analysis of extracts of various cell lines, using CTR9 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/CTR9-Antibody-Western-Blot-NBP3-04381-img0001.jpg "Western Blot: CTR9 Antibody [NBP3-04381] - Analysis of extracts of various cell lines, using CTR9 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")