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![Western Blot: CDK8 Antibody (7L5X2) [NBP3-16850] - Western blot analysis of extracts of various cell lines, using CDK8 Rabbit mAb (NBP3-16850) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/CDK8-Antibody-7L5X2-Western-Blot-NBP3-16850-img0001.jpg "Western Blot: CDK8 Antibody (7L5X2) [NBP3-16850] - Western blot analysis of extracts of various cell lines, using CDK8 Rabbit mAb (NBP3-16850) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western Blot: Cdk8 Overexpression Lysate (Adult Normal) [NBL1-09046] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cdk8.](http://images.novusbio.com/fullsize/Cdk8-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09046-img0002.jpg "Western Blot: Cdk8 Overexpression Lysate (Adult Normal) [NBL1-09046] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cdk8.")
![Immunoprecipitation:CDK8 AntibodyNBP3-29908] -Detection of human CDK8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDK8 antibody NBP3-29908 (lot NBP3-29908-2) used for IP at 6 µg per reaction. CDK8 was also immunoprecipitated by a previous lot of this antibody (lot NBP3-29908-1). For blotting immunoprecipitated CDK8, NBP3-29908 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29908_rabbit-cdk8-pab-301220241917281.jpg "Immunoprecipitation:CDK8 AntibodyNBP3-29908] -Detection of human CDK8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDK8 antibody NBP3-29908 (lot NBP3-29908-2) used for IP at 6 µg per reaction. CDK8 was also immunoprecipitated by a previous lot of this antibody (lot NBP3-29908-1). For blotting immunoprecipitated CDK8, NBP3-29908 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")