| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05409-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21552-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26428-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26666-50ul | |||
![Western Blot: CDK2 [p Tyr15] Antibody (SN72-04) [NBP2-67686] - Lane 1: Hela cells, whole cell lysate, 10ug/lane Lane 2: Hela cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-Cdk2(Y15) antibody at 1/500 dilution. Anti-Cdk2 antibody at 1/500 dilution. Anti-GAPDH antibody at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 34 kDa Observed band size: 34 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 30 seconds](http://images.novusbio.com/fullsize/CDK2-p-Tyr15-Antibody-SN72-04-Western-Blot-NBP2-67686-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: CDK2 [p Tyr15] Antibody (SN72-04) [NBP2-67686] - Lane 1: Hela cells, whole cell lysate, 10ug/lane Lane 2: Hela cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-Cdk2(Y15) antibody at 1/500 dilution. Anti-Cdk2 antibody at 1/500 dilution. Anti-GAPDH antibody at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 34 kDa Observed band size: 34 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 30 seconds")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: CDK2 [p Tyr15] Antibody (SN72-04) [NBP2-67686] - Analysis of paraffin-embedded mouse colon tissue using anti-Phospho-Cdk2(Y15) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CDK2-p-Tyr15-Antibody-SN72-04-Immunohistochemistry-Paraffin-NBP2-67686-img0002.jpg)
![Western Blot: CDK2 [p Tyr15] Antibody (SN72-04) [NBP2-67686] - Lane 1: Hela cell lysate (10 ug/Lane)Lane 2: NIH/3T3 cell lysate (10 ug/Lane)Lane 3: Mouse spleen tissue lysate (20 ug/Lane)Predicted band size: 34 kDa Observed band size: 35 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-52) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/CDK2-p-Tyr15-Antibody-SN72-04-Western-Blot-NBP2-67686-img0003.jpg)
![Immunohistochemistry-Paraffin: CDK2 [p Tyr15] Antibody (SN72-04) [NBP2-67686] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-Cdk2(Y15) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CDK2-p-Tyr15-Antibody-SN72-04-Immunohistochemistry-Paraffin-NBP2-67686-img0001.jpg)
![Western Blot: CDK2 [p Thr160] Antibody [NBP3-13301] - Wild-type (WT) and CDK2 (phospho Thr160) knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK2 (phospho Thr160) antibody (NBP3-13301) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/CDK2-[p-Thr160]-Antibody-Western-Blot-NBP3-13301-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CDK2 [p Thr160] Antibody [NBP3-13301] - Wild-type (WT) and CDK2 (phospho Thr160) knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK2 (phospho Thr160) antibody (NBP3-13301) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: CDK2 [p Thr160] Antibody [NBP3-13301] - CDK2 (phospho Thr160) antibody detects CDK2 (phospho Thr160) protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human colon. CDK2 (phospho Thr160) stained by CDK2 (phospho Thr160) antibody (NBP3-13301) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/CDK2-[p-Thr160]-Antibody-Immunohistochemistry-Paraffin-NBP3-13301-img0003.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: CDK2 [p Thr160] Antibody [NBP3-13301] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CDK2 (phospho Thr160) antibody (NBP3-13301) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CDK2-[p-Thr160]-Antibody-Western-Blot-NBP3-13301-img0001.jpg)
![Immunohistochemistry-Paraffin: CDK2 [p Thr160] Antibody [NBP3-13301] - CDK2 (phospho Thr160) antibody detects CDK2 (phospho Thr160) protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. CDK2 (phospho Thr160) stained by CDK2 (phospho Thr160) antibody (NBP3-13301) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/CDK2-[p-Thr160]-Antibody-Immunohistochemistry-Paraffin-NBP3-13301-img0002.jpg)
![Immunohistochemistry-Paraffin: CDK2 [p Thr160] Antibody [NBP3-05409] - Immunohistochemistry of paraffin-embedded human breast carcinoma using CDK2 antibody (NBP3-05409).](http://images.novusbio.com/fullsize/CDK2-[p-Thr160]-Antibody-Immunohistochemistry-Paraffin-NBP3-05409-img0001.jpg "Immunohistochemistry-Paraffin: CDK2 [p Thr160] Antibody [NBP3-05409] - Immunohistochemistry of paraffin-embedded human breast carcinoma using CDK2 antibody (NBP3-05409).")
![Western Blot: CDK2 [p Thr160] Antibody [NBP3-05409] - analysis of C6, using Phospho-CDK2-T160 antibody at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/antibody/nbp3-05409_rabbit-polyclonal-cdk2-p-thr160-antibody-western-blot-159202383355..jpg)
![Western Blot: CDK2 [p Thr160] Antibody - Azide and BSA Free [CDK2] - Western blot analysis of lysates from C6 cells using CDK2 Rabbit pAb at 1:1000 dilution. C6 cells were treated by Nocodazole (10 ng/ml) at 37C for 20 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 20 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-05409_rabbit-polyclonal-cdk2-p-thr160-antibody-52202516345255.jpg)
![Western Blot: CDK2 [p Tyr 15] Antibody (SR1560) [NBP3-21552] - Western blot analysis using NBP3-21552 in (1) HeLa treated with AP; (2) HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21552_rabbit-cdk2-p-tyr-15-mab-sr1560-57202317215182.jpg "<b>Biological Strategies Validation. </b>Western Blot: CDK2 [p Tyr 15] Antibody (SR1560) [NBP3-21552] - Western blot analysis using NBP3-21552 in (1) HeLa treated with AP; (2) HeLa cell lysate.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: CDK2 [p Tyr 15] Antibody (SR1560) [NBP3-21552] - Immunohistochemical analysis of paraffin-embedded human bladder cancer, using NBP3-21552.](http://images.novusbio.com/fullsize/nbp3-21552_rabbit-cdk2-p-tyr-15-mab-sr1560-5720231564720.jpg)
![Immunoprecipitation: CDK2 [p Tyr15] Antibody (2C4) [NBP3-26428] - Immunoprecipitating CDK2 [p Tyr15] in Hela whole cell lysate treated with Pervanadate.Lane 1: Rabbit control IgG (1ug)instead of NBP3-26428 in Hela whole cell lysate treated with Pervanadate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).Lane 2: NBP3-26428 (3ug) + Hela whole cell lysate treated with Pervanadate (1mg).Lane 3: Hela whole cell lysate treated with Pervanadate (20ug).](http://images.novusbio.com/fullsize/nbp3-26428_rabbit-cdk2-p-tyr-15-mab-2c4-262202413221513.jpg "<b>Biological Strategies Validation. </b>Immunoprecipitation: CDK2 [p Tyr15] Antibody (2C4) [NBP3-26428] - Immunoprecipitating CDK2 [p Tyr15] in Hela whole cell lysate treated with Pervanadate.Lane 1: Rabbit control IgG (1ug)instead of NBP3-26428 in Hela whole cell lysate treated with Pervanadate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).Lane 2: NBP3-26428 (3ug) + Hela whole cell lysate treated with Pervanadate (1mg).Lane 3: Hela whole cell lysate treated with Pervanadate (20ug).")
![<b>Biological Strategies Validation. </b>Western Blot: CDK2 [p Tyr15] Antibody (2C4) [NBP3-26428] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate (treated with Pervanadate or not). All lanes: CDK2 [p Tyr15] Antibody at 0.8ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.Predicted band size: 34 KDaObserved band size: 34 KDa](http://images.novusbio.com/fullsize/nbp3-26428_rabbit-cdk2-p-tyr-15-mab-2c4-28220241140949.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CDK2 [p Tyr15] Antibody (2C4) [NBP3-26428] - Analysis of CDK2 [p Tyr15] Antibody (2C4) diluted at 1:80 and staining in Hela cells (treated with Pervanadate). The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26428_rabbit-cdk2-p-tyr-15-mab-2c4-28220241312220.jpg)
![Immunohistochemistry: CDK2 Antibody (6A5) [NBP3-26666] - Image of CDK2 Antibody (6A5) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26666_rabbit-cdk2-mab-6a5-262202414471566.jpg "Immunohistochemistry: CDK2 Antibody (6A5) [NBP3-26666] - Image of CDK2 Antibody (6A5) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: CDK2 Antibody (6A5) [NBP3-26666] - Positive Western Blot detected in: K562 whole cell lysate, HEK293 whole cell lysate, NIH/3T3 whole cell lysate, Hela whole cell lysate, HL-60 whole cell lysate, A549 whole cell lysate. All lanes: CDK2 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 34, 31 kDa Observed band size: 34 kDa](http://images.novusbio.com/fullsize/nbp3-26666_rabbit-cdk2-mab-6a5-282202411351622.jpg)
![Western Blot: CDK2 Antibody (10E0N5) [NBP3-33184] - Western blot analysis of various lysates using CDK2 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-33184_rabbit-cdk2-mab-10e0n5-912202414154382.jpg "Western Blot: CDK2 Antibody (10E0N5) [NBP3-33184] - Western blot analysis of various lysates using CDK2 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")
![<b>Genetic Strategies Validation. </b>Western Blot: CDK2 Antibody (10E0N5) [NBP3-33184] - Western blot analysis of lysates from wild type(WT) and CDK2 knockout (KO) 293T cells, using [KO Validated] CDK2 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-33184_rabbit-cdk2-mab-10e0n5-822025209873.jpg)
![Western Blot: CDK2 [p Thr160] Antibody (ARC57166) [NBP3-33582] - Western Blot analysis of lysates from C6 cells using Phospho-CDK2-T160 Rabbit mAb at 1:1000 dilution. C6 cells were treated by Nocodazole (10ng/ml).Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-33582_rabbit-cdk2-p-thr160-mab-arc57166-31120251744450.jpg "<b>Biological Strategies Validation. </b>Western Blot: CDK2 [p Thr160] Antibody (ARC57166) [NBP3-33582] - Western Blot analysis of lysates from C6 cells using Phospho-CDK2-T160 Rabbit mAb at 1:1000 dilution. C6 cells were treated by Nocodazole (10ng/ml).Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.")
![<b>Biological Strategies Validation. </b>Western Blot: CDK2 [p Thr160] Antibody (ARC57166) [NBP3-33582] - Western Blot analysis of lysates from HeLa cells using Phospho-CDK2-T160 Rabbit mAb at 1:1000 dilution. HeLa cells were treated by Nocodazole (20ng/ml).Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-33582_rabbit-cdk2-p-thr160-mab-arc57166-31120251744481.jpg)
![Western Blot: CDK2 [p Tyr15] Antibody (7Z5H5) [NBP3-33556] - Western blot analysis of lysates from NIH/3T3 cells, using CDK2 Rabbit mAb at 1:1000 dilution. NIH/3T3 cells were treated by Hydroxyurea (4 Mm) at 37C for 20 hours or treated by Nocodazole (100 ng/mL) at 37C for 24 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-33556_rabbit-cdk2-p-tyr15-mab-7z5h5-91220241519583.jpg "Western Blot: CDK2 [p Tyr15] Antibody (7Z5H5) [NBP3-33556] - Western blot analysis of lysates from NIH/3T3 cells, using CDK2 Rabbit mAb at 1:1000 dilution. NIH/3T3 cells were treated by Hydroxyurea (4 Mm) at 37C for 20 hours or treated by Nocodazole (100 ng/mL) at 37C for 24 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 3s.")
