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![Western Blot: CCL4/MIP-1 beta Antibody (SR1111) [NBP3-21790] - Western blot analysis using NBP3-21790 in (1) THP-1 cell lysate; (2) THP-1 cell treated with PMA+LPS+Brefeldin A.](http://images.novusbio.com/fullsize/nbp3-21790_rabbit-ccl4-mip-1-beta-mab-sr1111-57202315234914.jpg "Western Blot: CCL4/MIP-1 beta Antibody (SR1111) [NBP3-21790] - Western blot analysis using NBP3-21790 in (1) THP-1 cell lysate; (2) THP-1 cell treated with PMA+LPS+Brefeldin A.")
![Western Blot: CCL4L1/LAG-1 Antibody [NBP1-77897] - Shows detection of recombinant swine MIP-1b (CCL4) raised in yeast. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 4ug/mL primary antibody overnight at 4C. After washing, membrane was probed with IRDye800 (TM) conjugated Goat Anti-Rabbit IgG at 1:20,000 for 45 min at room temperature.](http://images.novusbio.com/fullsize/CCL4L1-LAG-1-Antibody-Western-Blot-NBP1-77897-img0002.jpg "Western Blot: CCL4L1/LAG-1 Antibody [NBP1-77897] - Shows detection of recombinant swine MIP-1b (CCL4) raised in yeast. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 4ug/mL primary antibody overnight at 4C. After washing, membrane was probed with IRDye800 (TM) conjugated Goat Anti-Rabbit IgG at 1:20,000 for 45 min at room temperature.")
![Western Blot: CCL4/MIP-1 beta Antibody (JE33-21) [NBP3-32115] - Western blot analysis of CCL4/MIP-1 beta on different lysates with Rabbit anti-CCL4/MIP-1 beta antibody (NBP3-32115) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours cell lysate Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 10 kDa Observed band size: 10 kDa Exposure time: 1 minute 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32115) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32115_rabbit-ccl4-mip-1-beta-mab-je33-21-278202416331237.jpg "Western Blot: CCL4/MIP-1 beta Antibody (JE33-21) [NBP3-32115] - Western blot analysis of CCL4/MIP-1 beta on different lysates with Rabbit anti-CCL4/MIP-1 beta antibody (NBP3-32115) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours cell lysate Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 treated with 100nM TPA overnight then add 100ng/mL LPS for 7 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 10 kDa Observed band size: 10 kDa Exposure time: 1 minute 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32115) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![ELISA: Rat CCL4/MIP-1 beta ELISA Kit (Colorimetric) [NBP3-08149] - Samples were spiked with high concentrations of Rat CCL4/MIP-1 beta and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-08149_rat-ccl4-mip-1-beta-elisa-kit-colorimetric-132202416254035.jpg "ELISA: Rat CCL4/MIP-1 beta ELISA Kit (Colorimetric) [NBP3-08149] - Samples were spiked with high concentrations of Rat CCL4/MIP-1 beta and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat CCL4/MIP-1 beta ELISA Kit (Colorimetric) [NBP3-08149] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-08149_rat-ccl4-mip-1-beta-elisa-kit-colorimetric-4320248574395.jpg)
![Western Blot: Macrophage Inflammatory Protein 1 beta Overexpression Lysate (Adult Normal) [NBL1-08858] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Macrophage Inflammatory Protein 1 beta.](http://images.novusbio.com/fullsize/Macrophage-Inflammatory-Protein-1-beta-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-08858-img0002.jpg "Western Blot: Macrophage Inflammatory Protein 1 beta Overexpression Lysate (Adult Normal) [NBL1-08858] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Macrophage Inflammatory Protein 1 beta.")
![Western Blot: CCL4/MIP-1 beta Antibody [NBP3-05561] - Western blot analysis of extracts of various cell lines, using CCL4/MIP-1 beta antibody (NBP3-05561) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/CCL4-MIP-1-beta-Antibody-Western-Blot-NBP3-05561-img0001.jpg "Western Blot: CCL4/MIP-1 beta Antibody [NBP3-05561] - Western blot analysis of extracts of various cell lines, using CCL4/MIP-1 beta antibody (NBP3-05561) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")