Caspase-1: Novus Biologicals

NB100-56565

Caspase-1 Antibody (14F468) - BSA Free

(73 Publications)

0.1 mg

NB100-56565

Simple Western lane view shows a specific band for Caspase 1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

<b>Biological Strategies Validation. </b>Caspase-1-Antibody-14F468-Western-Blot-NB100-56565-img0015.jpg

Caspase-1-Antibody-14F468-Western-Blot-NB100-56565-img0017.jpg

<b>Biological Strategies Validation. </b>Caspase-1-Antibody-14F468-Immunohistochemistry-NB100-56565-img0020.jpg

Caspase-1-Antibody-14F468-Western-Blot-NB100-56565-img0016.jpg

Analysis of Caspase-1 using a Caspase-1 monoclonal antibody. Human HeLa (A) and mouse NIH3T3 lysate probed with Caspase-1 antibody at 0.5 ug/ml and 2 ug/ml, respectively.

Adenocarcinoma of the rectum stained with Caspase-1 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Detection of Caspase-1 protein in a section of normal human colon using 5 ug/ml concentration of Caspase 1 antibody (clone 14F468). Distinct cytoplasmic staining along with some nuclear positivity was observed in crypts/mucosa, and staining was found to be more intense in the absorptive columnar epithelial cells. [10X Magnification]

Normal lung from human using 5 ug/ml concentration of Caspase 1 antibody (clone 14F468). In this representative lung section, different type of cells including pseudostratified columnar epithelium of bronchiole and the simple squamous epithelium of alveoli may be seen to develop immunoreactivity for Caspase 1. [10X Magnification]

Normal skin from human using 5 ug/ml concentration of Caspase 1 antibody (clone 14F468). Strong cytoplasmic/nuclear staining developed in all the epidermal cells, blood vessels and some cells of the dermal connective tissues layer. [10X Magnification]

Detection of Caspase-1 in a section of human ovarian cancer using 5 ug/ml concentration of Caspase 1 antibody (clone 14F468).

Tissue section of human intestine using Caspase-1 antibody (clone 14F468) at 5ug/ml concentration (1:200 dilution). The primary antibody binding to Caspase 1 in cells was detected using HRP conjugated anti-Mouse secondary antibody with DAB reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This Caspase 1 antibody generated a diffused but specific cytoplasmic staining in columnar epithelia cells of villi, and a few cells depicted nuclear staining also. Only a subset of connective tissue cells in lamina propria depicted positivity (cytoplasmic) for this protein.

Western Blot: Caspase-1 Antibody (14F468) - BSA Free [NB100-56565] - Glucose fluctuations promoted the activation of NLRP3 inflammasome. (A) Immunofluorescence staining of NLRP3-ASC interaction in rat hearts. The heart sections were labeled with anti-NLRP3 (red), anti-ASC (green), & DAPI (blue) (n = 3 per group). (B–D) The mRNA levels of NLRP3, IL-1 beta & IL-18 in rat hearts of the three groups (n = 4 per group). (E–H) The protein expressions of NLRP3, ASC & cleaved caspase-1 in rat hearts of the three groups (n = 4 per group). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35592403), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Caspase-1 Antibody (14F468) - BSA Free [NB100-56565] - Caspase-1 expression increases with age in human fibroblasts: a Immunoblot analysis of caspase-1 & ASC in human fibroblasts obtained from a donor at the ages of 49, 52 & 64 y/o. b Immunocytochemistry image of human fibroblasts stained for caspase-1 (green) & Phalloidin (red) from a donor at three different ages (49, 52 & 64 y/o). Bar graph: 100 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30473634), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Caspase-1 Antibody (14F468) - BSA Free [NB100-56565] - Aging alters expression of NLRP1 inflammasome components. Representative immunoblots & densitometric analysis of immunoblots for caspase-1 (Casp1), caspase-11 (Casp11), NLRP1, ASC, cleaved XIAP, P2X7 receptor (P2X7R) & pannexin-1 (PanX1) in brain lysates of young (Y) & aged (A) animals. Protein levels of cleaved caspase-1 & -11 are higher in aged animals compared to young. Protein levels of NLRP1 & ASC did not change with age whereas P2X7 receptor, the pannexin-1 protein & the cleaved fragment of XIAP are higher in aged animals than in young animals. beta -Tubulin was used as an internal standard & control for protein loading. Data are presented as mean +/- SEM. *p < 0.05, **p < 0.01, ***p < 0.005 compared with young. n = 6 per group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22133203), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Caspase-1 Antibody (14F468) - BSA Free [NB100-56565] - Role of NF-kappa B in glucose fluctuation-induced inflammasome-related myocardial fibrosis. (A) Immunofluorescence staining of NF-kappa B/p65 nucleation (n = 3 per group). (B,C) The phosphorylation level of NF-kappa B/p65 in rat hearts of the three groups (n = 4 per group). (D) Immunofluorescence staining of intracellular NLRP3–ASC interaction in the GF & GF + TPCA-1 groups. The NRCFs were labeled with anti-NLRP3 (red), anti-ASC (green), & DAPI (blue) (n = 3 per group). (E–G) After application of TPCA-1 (0.5 μM), the protein expressions of cleaved caspase-1 & collagen I were measured by western blot (n = 3 per group). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35592403), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Western Blot: Caspase-1 Antibody (14F468) - BSA Free [NB100-56565] - Inflammasome proteins are elevated in the cytosolic fraction of aged mice: a Representative image of immunoblot analyses of inflammasome proteins in the cytosolic fraction of the cortex of young (Y) & aged (A) mice. Quantification of immunoblot analysis of NLRC4 (b), caspase-1 (c), ASC (d), IL-18 (e) in the cortex. a Representative image of immunoblot analyses of inflammasome proteins in the cytosolic fraction of the hippocampus of young (Y) & aged (A) mice. Quantification of immunoblot analysis of NLRC4 (g), caspase-1 (h), caspase-11 (i), ASC (j), IL-1 beta (k) in the hippocampus. Data presented as mean+/-SEM. N = 5 per group. *p < 0.05 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30473634), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Western Blot: Caspase-1 Antibody (14F468) - BSA Free [NB100-56565] - NLRP3 inhibition with MCC950 reversed myocardial fibrosis induced by glucose fluctuations. (A) Representative images of hematoxylin & eosin (HE) staining for myocardial tissue in the FBG + NaCl & FBG + MCC950 groups (n = 3 per group). (B–C) Representative images of myocardial fibrosis & fibrosis area (%) in the FBG + NaCl & FBG + MCC950 groups (n = 4 per group). (D–K) The protein expressions of NLRP3, cleaved caspase-1, TGF-beta 1, collagen I & collagen III in the FBG + NaCl & FBG + MCC950 groups (n = 6 per group). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35592403), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Western Blot: Caspase-1 Antibody (14F468) - BSA Free [NB100-56565] - Inflammasome proteins are elevated in the cytosolic fraction of aged mice: a Representative image of immunoblot analyses of inflammasome proteins in the cytosolic fraction of the cortex of young (Y) & aged (A) mice. Quantification of immunoblot analysis of NLRC4 (b), caspase-1 (c), ASC (d), IL-18 (e) in the cortex. a Representative image of immunoblot analyses of inflammasome proteins in the cytosolic fraction of the hippocampus of young (Y) & aged (A) mice. Quantification of immunoblot analysis of NLRC4 (g), caspase-1 (h), caspase-11 (i), ASC (j), IL-1 beta (k) in the hippocampus. Data presented as mean+/-SEM. N = 5 per group. *p < 0.05 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30473634), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Western Blot: Caspase-1 Antibody (14F468) - BSA Free [NB100-56565] - Role of inflammasome in glucose fluctuation-induced myocardial fibrosis. (A) Immunofluorescence staining of intracellular NLRP3–ASC interaction examined by confocal microscopy. The NRCFs were labeled with anti-NLRP3 (red), anti-ASC (green), & DAPI (blue) (n = 3 per group). (B–G) After siRNA targeting NLRP3 transfection, the protein expressions of NLRP3, cleaved caspase-1 & collagen I were measured by western blot (n = 3 per group). (H) Representative images of immunofluorescence staining of intracellular alpha -SMA in the NG, HG, GF, & GF + siRNA groups (n = 3 per group). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35592403), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Caspase-1 Antibody (14F468) - BSA Free [NB100-56565] - Epirubicin suppresses the NLRP3 inflammasome in presence of autophagy inhibitors. (A) LPS/ATP-induced IL-1 beta secretion from PM in the absence or presence of bafilomycin & 3-MA is shown. Means ± SEM from 10 (IL-1 beta ) independent experiments are shown. (B) Representative immunoblot of LC3b after epirubicin & LPS/ATP treatments. (C,D) Representative immunoblots of inflammasome & autophagy components after inhibition with (C) bafilomycin or (D) 3-MA in PM whole cell lysates. Cells were treated with indicated concentrations of epirubicin treatment over 24 h, followed by LPS (10 ng/mL) in the absence or presence of bafilomycin (300 nM) or 3-MA (10 mM) for 3 h & NLRP3 activation by ATP (1 mM, 1 h). Whole cell lysates were subjected to SDS-PAGE (10% gel) & processed for immunoblot analysis with specific antibodies. Here, beta -actin served as loading control. Statistical tests: one-sample t-test (A) ** p < 0.01, *** p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31905600), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Western Blot: Caspase-1 Antibody (14F468) - BSA Free [NB100-56565] - Genetic or pharmacologic disruption of Caspase 1 blocks LukAB-induced cytokine secretion but not cell death.(A) THP1 cells were transfected with siRNA against Caspase 1, ASC or a non-targeting sequence. Cell lysates were collected & analyzed by immunoblot to confirm knockdown of pro-Caspase 1 & ASC. (B) THP1 siRNA cells were incubated with propidium iodide & intoxicated with 50 ng/mL LukAB for 1 hour then analyzed by flow cytometry. (C & D) THP1 siRNA cells were either primed with LTA (500ng/mL) (C) or untreated (D) then intoxicated with LukAB (50ng/mL) for 1 hr before culture supernatants were analyzed for release of IL-1 beta (C) or IL-18 (D). (E & F) Primary CD14+ human monocytes were incubated with the indicated concentration of z-YVAD-FMK or VX-765 for 30 minutes. Primary monocytes were incubated in the presence of propidium iodide (E) then intoxicated with LukAB (50ng/mL) & analyzed by flow cytometry. (F) Primary monocytes were intoxicated with LukAB (50ng/mL) & culture supernatants were analyzed for release of IL-18. Propidium iodide staining & IL-18 secretion are reported as a fraction of measurement in primary cells not treated with inhibitor. Bars represent the mean ± standard error of the mean for at least two independent experiments, each performed in triplicate. Asterisks indicate significance at a p-value of ≤ 0.05 by Tukey’s multiple comparisons post-test for 1-way or 2-way ANOVA, as appropriate. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1004970), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Western Blot: Caspase-1 Antibody (14F468) - BSA Free [NB100-56565] - Probenecid reduces protein expression of NLRP1 inflammasome & ameliorates spatial learning deficits in aged rats. (A) Representative immunoblots of cleaved caspase-1, pannexin1 & P2X7R in hippocampal lysates of vehicle (Veh)-treated & probenecid (Pr)-treated 18-month-old rats. beta -tubulin was used as an internal control. (B) Densitometric analysis of immunoblots from brain lysates of cleaved caspase-1 (Casp1), P2X7 receptor (P2X7R), & pannexin1 (PanX1). (C-D) Aged animals underwent behavioral testing following either probenecid or vehicle treatment. (C) In a hippocampal-dependent spatial learning task via Morris water maze, latency to platform was measured on days 1-3 & 8-10. Probenecid-treatment improved latency to platform measured on the final day of testing (D) Mean path length was determined on day 10 of testing & probenecid-treated rats demonstrated significantly shorter mean path lengths than vehicle-treated controls. Drug treatment was administered twice daily for 3 days (days 7-9). Data are presented as mean +/- SEM *p < 0.05, **p < 0.005 compared to vehicle. N = 6-8/per group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22133203), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-5028

Alt Catalog# IMG-5028

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-56565AF350
Alexa Fluor 405	NB100-56565AF405
Alexa Fluor 488	NB100-56565AF488
Alexa Fluor 532	NB100-56565AF532
Alexa Fluor 594	NB100-56565AF594
Alexa Fluor 647	NB100-56565AF647
Alexa Fluor 700	NB100-56565AF700
Alexa Fluor 750	NB100-56565AF750
Alexa Fluor Plus 405	NB100-56565AFP405
Alexa Fluor Plus 488	NB100-56565AFP488
Alexa Fluor Plus 555	NB100-56565AFP555
Alexa Fluor Plus 594	NB100-56565AFP594
Alexa Fluor Plus 647	NB100-56565AFP647
Alexa Fluor Plus 680	NB100-56565AFP680
Allophycocyanin	NB100-56565APC
Biotin	NB100-56565B
CoraFluor 1	NB100-56565CL1
DyLight 350	NB100-56565UV
DyLight 405	NB100-56565V
DyLight 488	NB100-56565G
DyLight 550	NB100-56565R
DyLight 594	NB100-56565DL594
DyLight 650	NB100-56565C
DyLight 680	NB100-56565FR
DyLight 755	NB100-56565IR
FITC	NB100-56565F
HRP	NB100-56565H
Janelia Fluor 525	NB100-56565JF525
Janelia Fluor 549	NB100-56565JF549
Janelia Fluor 646	NB100-56565JF646
Janelia Fluor 669	NB100-56565JF669
PE	NB100-56565PE
PerCP	NB100-56565PCP
mFluor Violet 450 SE	NB100-56565MFV450
mFluor Violet 500 SE	NB100-56565MFV500
mFluor Violet 610 SE	NB100-56565MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-56565SS
Azide and BSA Free	NBP2-33230

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DCA100

Human Caspase-1/ICE Quantikine ELISA Kit

(47 Publications)

1 Kit

DCA100

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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MAB6215

Caspase-1 Antibody (661228) [Unconjugated]

(20 Publications)

100 μg

MAB6215

HAF007). A specific band was detected for Caspase-1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_lightbox">

Western blot shows lysates of A431 human epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Caspase-1 Monoclonal Antibody (Catalog # MAB6215) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF007). A specific band was detected for Caspase-1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of A431 human epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Caspase-1 Monoclonal Antibody (Catalog # MAB6215) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Caspase-1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

Caspase-1 was detected in immersion fixed THP-1 human acute monocytic leukemia cell line using Mouse Anti-Human Caspase-1 Monoclonal Antibody (Catalog # MAB6215) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB6215U-100UG
Alexa Fluor 405	FAB6215V-100UG
Alexa Fluor 488	FAB6215G-100UG
Alexa Fluor 532	FAB6215X-100UG
Alexa Fluor 594	FAB6215T-100UG
Alexa Fluor 647	FAB6215R-100UG
Alexa Fluor 700	FAB6215N-100UG
Alexa Fluor 750	FAB6215S-100UG
Alexa Fluor Plus 405	FAB6215AFP405-100UG
Alexa Fluor Plus 488	FAB6215AFP488-100UG
Alexa Fluor Plus 555	FAB6215AFP555-100UG
Alexa Fluor Plus 594	FAB6215AFP594-100UG
Alexa Fluor Plus 647	FAB6215AFP647-100UG
Alexa Fluor Plus 680	FAB6215AFP680-100UG
Allophycocyanin	FAB6215A
Biotin	FAB6215B
CoraFluor 1	FAB6215CL1
DyLight 350	FAB6215D
DyLight 405	FAB6215E
DyLight 488	FAB6215K
DyLight 550	FAB6215L
DyLight 594	FAB6215M
DyLight 650	FAB6215W
DyLight 680	FAB6215Y
DyLight 755	FAB6215Z
FITC	FAB6215F
HRP	FAB6215H
Janelia Fluor 525	FAB6215JF525
Janelia Fluor 549	FAB6215I
Janelia Fluor 646	FAB6215J
Janelia Fluor 669	FAB6215JF669
PE	FAB6215P
PerCP	FAB6215C
mFluor Violet 450 SE	FAB6215MFV450
mFluor Violet 500 SE	FAB6215MFV500
mFluor Violet 610 SE	FAB6215MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB6215-SP

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NB100-56564

Caspase-1 Antibody - BSA Free

(3 Publications)

0.1 mg

NB100-56564

Immunohistochemistry-Paraffin: Caspase-1 Antibody [NB100-56564] - Human reactive lymph node stained for Caspase-1 expression (NB100-56564) using a DAB chromogen and Hematoxylin counterstain. B is a higher magnification of A. Staining in macrophages is observed.

Sevoflurane can increase the protein expression of NLRP3 inflammasome and its downstream inflammatory factors in prefrontal cortex. A–E Expression levels of NLRP3 inflammatory factors, related components, and downstream cytokine proteins. Western blot images were cropped to remove irrelevant sections of the image and display only the proteins of interest. Loading control: protein extracted from prefrontal cortex tissue from sham subjects treated with saline (lane1). Tissue used for Western blot analysis was collected 24-h after the last drug-administration. F–I NLRP3 inflammatory factors, related components, and downstream cytokine PCR results. Data are expressed as mean +/- SD; n = 4 for each experimental group. ***Compared with the Ctrl group, ###compared with the Sev group. P < 0.05 indicated a statistically significant difference Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36709248), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-5025

Alt Catalog# IMG-5025

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-56564SS

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AF6215

Caspase-1 Antibody [Unconjugated]

(5 Publications)

100 μg

AF6215

HAF109). A specific band was detected for Caspase-1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_lightbox">

Western blot shows lysates of A431 human epithelial carcinoma cell line and THP-1 human acute monocytic leukemia cell line. PVDF Membrane was probed with 0.25 µg/mL of Goat Anti-Human Caspase-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6215) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). A specific band was detected for Caspase-1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of A431 human epithelial carcinoma cell line and THP-1 human acute monocytic leukemia cell line. PVDF Membrane was probed with 0.25 µg/mL of Goat Anti-Human Caspase-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6215) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Caspase-1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

Caspase-1 was detected in immersion fixed THP-1 human acute monocytic leukemia cell line using Goat Anti-Human Caspase-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6215) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

<b>Genetic Strategies Validation. </b>Simple Western lane view shows lysates of A431 human epithelial carcinoma parental cell line and Caspase-1 knockout A431 cell line (KO), loaded at 0.2 mg/mL. A specific band was detected for Caspase-1 at approximately 52 kDa (as indicated) using 25 µg/mL of Goat Anti-Human Caspase-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6215) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

HAF017). A specific band was detected for Caspase-1 at approximately 50 kDa (as indicated) in the parental A431 cell line, but is not detectable in knockout A431 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Genetic Strategies Validation. </b>Western blot shows lysates of A431 human epithelial carcinoma parental cell line and Caspase-1 knockout A431 cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Caspase-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6215) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for Caspase-1 at approximately 50 kDa (as indicated) in the parental A431 cell line, but is not detectable in knockout A431 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF6215U-100UG
Alexa Fluor 405	AF6215V-100UG
Alexa Fluor 488	AF6215G-100UG
Alexa Fluor 532	AF6215X-100UG
Alexa Fluor 594	AF6215T-100UG
Alexa Fluor 647	AF6215R-100UG
Alexa Fluor 700	AF6215N-100UG
Alexa Fluor 750	AF6215S-100UG
Alexa Fluor Plus 405	AF6215AFP405-100UG
Alexa Fluor Plus 488	AF6215AFP488-100UG
Alexa Fluor Plus 555	AF6215AFP555-100UG
Alexa Fluor Plus 594	AF6215AFP594-100UG
Alexa Fluor Plus 647	AF6215AFP647-100UG
Alexa Fluor Plus 680	AF6215AFP680-100UG
Allophycocyanin	AF6215A
Biotin	AF6215B
CoraFluor 1	AF6215CL1
DyLight 350	AF6215D
DyLight 405	AF6215E
DyLight 488	AF6215K
DyLight 550	AF6215L
DyLight 594	AF6215M
DyLight 650	AF6215W
DyLight 680	AF6215Y
DyLight 755	AF6215Z
FITC	AF6215F
HRP	AF6215H
Janelia Fluor 525	AF6215JF525
Janelia Fluor 549	AF6215I
Janelia Fluor 646	AF6215J
Janelia Fluor 669	AF6215JF669
PE	AF6215P
PerCP	AF6215C
mFluor Violet 450 SE	AF6215MFV450
mFluor Violet 500 SE	AF6215MFV500
mFluor Violet 610 SE	AF6215MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF6215-SP

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NBP2-75014

Mouse Caspase-1 ELISA Kit (Colorimetric)

(6 Publications)

1 Kit

NBP2-75014

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Standard Curve:

31.25 - 2000 pg/mL

Sensitivity:

18.75 pg/mL

Sample Type:

Serum, plasma and other biological fluids

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NBP2-15713

Caspase-1 Antibody - BSA Free

(3 Publications)

0.1 ml

NBP2-15713

Immunohistochemistry-Paraffin: Caspase-1 Antibody [NBP2-15713] - Mouse kidney. Caspase 1 stained by Caspase 1 antibody [N1N3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: Caspase-1 Antibody [NBP2-15713] - SW480 xenograft, using Caspase 1 alpha antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP1-76605

Caspase-1 Antibody - BSA Free

(1 Publication)

0.1 mg

NBP1-76605

The impact of Gent on TNF-alpha -induced NLRP3 inflammasome activation in RA-FLS. (A, B) NLRP3 and caspase-1 levels in RA-FLS were assessed via immunofluorescence microscopy. Nuclei were stained with DAPI (blue) while NLRP3 and caspase-1 immunofluorescence were in green (scale bar = 50 um). (C) NLRP3, ASC, and caspase-1 levels were assessed via Western blotting in RA-FLS, with GAPDH as a loading control. Data were presented as the mean +/- SD from three independent experiments. #P < 0.05, ##P < 0.01, and ###P < 0.001 vs. control; *P < 0.05, **P < 0.01 vs. TNF-alpha group. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32477105), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-5554

Alt Catalog# IMG-5554

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-76605AF350
Alexa Fluor 405	NBP1-76605AF405
Alexa Fluor 488	NBP1-76605AF488
Alexa Fluor 532	NBP1-76605AF532
Alexa Fluor 594	NBP1-76605AF594
Alexa Fluor 647	NBP1-76605AF647
Alexa Fluor 700	NBP1-76605AF700
Alexa Fluor 750	NBP1-76605AF750
Alexa Fluor Plus 405	NBP1-76605AFP405
Alexa Fluor Plus 488	NBP1-76605AFP488
Alexa Fluor Plus 555	NBP1-76605AFP555
Alexa Fluor Plus 594	NBP1-76605AFP594
Alexa Fluor Plus 647	NBP1-76605AFP647
Alexa Fluor Plus 680	NBP1-76605AFP680
Allophycocyanin	NBP1-76605APC
Biotin	NBP1-76605B
CoraFluor 1	NBP1-76605CL1
DyLight 350	NBP1-76605UV
DyLight 405	NBP1-76605V
DyLight 488	NBP1-76605G
DyLight 550	NBP1-76605R
DyLight 594	NBP1-76605DL594
DyLight 650	NBP1-76605C
DyLight 680	NBP1-76605FR
DyLight 755	NBP1-76605IR
FITC	NBP1-76605F
HRP	NBP1-76605H
Janelia Fluor 525	NBP1-76605JF525
Janelia Fluor 549	NBP1-76605JF549
Janelia Fluor 646	NBP1-76605JF646
Janelia Fluor 669	NBP1-76605JF669
PE	NBP1-76605PE
PerCP	NBP1-76605PCP
mFluor Violet 450 SE	NBP1-76605MFV450
mFluor Violet 500 SE	NBP1-76605MFV500
mFluor Violet 610 SE	NBP1-76605MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-76605-0.025mg

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NBP1-87680

Caspase-1 Antibody - BSA Free

0.1 ml

NBP1-87680

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-87680-25ul

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NBP3-13233

Caspase-1 Antibody - (cleaved) - BSA Free

(1 Publication)

100 ul

NBP3-13233

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NB100-56564PEP

Caspase-1 Antibody Blocking Peptide

0.1 mg

Applications:

Applications:

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H00000834-Q01

Recombinant Human Caspase-1 GST (N-Term) Protein

10 μg

H00000834-Q01

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Kit Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

CASP1 Products By Gene Name

Caspase-1 Antibody (14F468) - BSA Free

Human Caspase-1/ICE Quantikine ELISA Kit

Caspase-1 Antibody (661228) [Unconjugated]

Caspase-1 Antibody - BSA Free

Caspase-1 Antibody [Unconjugated]

Mouse Caspase-1 ELISA Kit (Colorimetric)

Caspase-1 Antibody - BSA Free

Caspase-1 Antibody - BSA Free

Caspase-1 Antibody - BSA Free

Caspase-1 Antibody - (cleaved) - BSA Free

Caspase-1 Antibody Blocking Peptide

Recombinant Human Caspase-1 GST (N-Term) Protein