| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-56000-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35231-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-97067-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-69023-25ul | |||
![Western Blot: Atlastin-3 Antibody (PSH02-47) [NBP3-32060] - Western blot analysis of Atlastin-3 on different lysates with Rabbit anti-Atlastin-3 antibody (NBP3-32060) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: A549 cell lysate Lane 4: HepG2 cell lysate Lane 5: COS-1 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: RAW264.7 cell lysate Lane 8: Neuro-2a cell lysate Lane 9: PC-12 cell lysate Lane 10: L6 cell lysate Lane 11: Mouse liver tissue lysate Lane 12: Rat liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32060) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32060_rabbit-atlastin-3-mab-psh02-47-27820241631612.jpg "Western Blot: Atlastin-3 Antibody (PSH02-47) [NBP3-32060] - Western blot analysis of Atlastin-3 on different lysates with Rabbit anti-Atlastin-3 antibody (NBP3-32060) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: A549 cell lysate Lane 4: HepG2 cell lysate Lane 5: COS-1 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: RAW264.7 cell lysate Lane 8: Neuro-2a cell lysate Lane 9: PC-12 cell lysate Lane 10: L6 cell lysate Lane 11: Mouse liver tissue lysate Lane 12: Rat liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32060) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Atlastin-3 Antibody (PSH02-47) [NBP3-32060] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Atlastin-3 antibody (NBP3-32060) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32060) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32060_rabbit-atlastin-3-mab-psh02-47-298202413585540.jpg)
![Immunocytochemistry/ Immunofluorescence: Atlastin-3 Antibody (PSH02-47) [NBP3-32060] - Immunocytochemistry analysis of HeLa cells labeling Atlastin-3 with Rabbit anti-Atlastin-3 antibody (NBP3-32060) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Atlastin-3 antibody (NBP3-32060) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32060_rabbit-atlastin-3-mab-psh02-47-29202410484884.jpg)
![Flow Cytometry: Atlastin-3 Antibody (PSH02-47) [NBP3-32060] - Flow cytometric analysis of HeLa cells labeling Atlastin-3. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32060, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32060_rabbit-atlastin-3-mab-psh02-47-2920241645326.jpg)
![Western Blot: Atlastin-3 Antibody [NBP2-15508] - Sample (30 ug of whole cell lysate) A: NT2D1 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Atlastin-3-Antibody-Western-Blot-NBP2-15508-img0001.jpg "Western Blot: Atlastin-3 Antibody [NBP2-15508] - Sample (30 ug of whole cell lysate) A: NT2D1 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Atlastin-3 Antibody [NBP2-15508] - Paraffin-embedded rat liver. ATL3 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Atlastin-3-Antibody-Immunohistochemistry-Paraffin-NBP2-15508-img0004.jpg)
![Immunohistochemistry-Paraffin: Atlastin-3 Antibody [NBP2-15508] - Paraffin-embedded rat cervix. ATL3 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Atlastin-3-Antibody-Immunohistochemistry-Paraffin-NBP2-15508-img0002.jpg)
![Immunohistochemistry-Paraffin: Atlastin-3 Antibody [NBP2-15508] - Paraffin-embedded rat brain. ATL3 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Atlastin-3-Antibody-Immunohistochemistry-Paraffin-NBP2-15508-img0003.jpg)
![Western Blot: Atlastin-3 Antibody [NBP2-56000] - Analysis in human cell line RT-4.](http://images.novusbio.com/fullsize/Atlastin-3-Antibody-Western-Blot-NBP2-56000-img0002.jpg "Western Blot: Atlastin-3 Antibody [NBP2-56000] - Analysis in human cell line RT-4.")
![Immunocytochemistry/Immunofluorescence: Atlastin-3 Antibody [NBP2-56000] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum.](http://images.novusbio.com/fullsize/Atlastin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56000-img0001.jpg)
![Western Blot: Atlastin-3 Antibody [NBP3-35231] - Western blot analysis of various lysates using Atlastin-3 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-35231_rabbit-atlastin-3-pab-912202415133419.jpg "Western Blot: Atlastin-3 Antibody [NBP3-35231] - Western blot analysis of various lysates using Atlastin-3 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.")
![Western Blot:[NBP3-29125]-Detection of human and mouse Atlastin-3 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , Jurkat , HEK293T and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-Atlastin-3 antibody used for WB at 0. 04 µg/ml and 1 µg/ml and used for IP at 6 µg/mg lysate. Atlastin-3 was also immunoprecipitated by rabbit anti-Atlastin-3 antibody , which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds and 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29125_rabbit-atlastin-3-pab-231202512101644.jpg "Western Blot:[NBP3-29125]-Detection of human and mouse Atlastin-3 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , Jurkat , HEK293T and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-Atlastin-3 antibody used for WB at 0. 04 µg/ml and 1 µg/ml and used for IP at 6 µg/mg lysate. Atlastin-3 was also immunoprecipitated by rabbit anti-Atlastin-3 antibody , which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds and 10 seconds.")
![Western Blot:[NBP3-29579]-Detection of human and mouse Atlastin-3 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , Jurkat , HEK293T and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-Atlastin-3 antibody used for WB at 0. 04 µg/ml and 1 µg/ml and used for IP at 6 µg/mg lysate. Atlastin-3 was also immunoprecipitated by rabbit anti-Atlastin-3 antibody , which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds and 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29579_rabbit-atlastin-3-pab-23120251252870.jpg "Western Blot:[NBP3-29579]-Detection of human and mouse Atlastin-3 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , Jurkat , HEK293T and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-Atlastin-3 antibody used for WB at 0. 04 µg/ml and 1 µg/ml and used for IP at 6 µg/mg lysate. Atlastin-3 was also immunoprecipitated by rabbit anti-Atlastin-3 antibody , which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds and 10 seconds.")
![Western Blot: Atlastin-3 Antibody [NBP2-97067] - Anti-Atlastin-3 rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse liver Tissue Lysate. Lane B: 293T Whole Cell Lysate. Lane C: NIH-3T3 Whole Cell Lysate. Lane D: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 61 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Atlastin-3-Antibody-Western-Blot-NBP2-97067-img0004.jpg "Western Blot: Atlastin-3 Antibody [NBP2-97067] - Anti-Atlastin-3 rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse liver Tissue Lysate. Lane B: 293T Whole Cell Lysate. Lane C: NIH-3T3 Whole Cell Lysate. Lane D: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 61 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: Atlastin-3 Antibody [NBP2-97067] - Atlastin-3 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 2 ul anti-Atlastin-3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Atlastin-3 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 61 kDa](http://images.novusbio.com/fullsize/Atlastin-3-Antibody-Immunoprecipitation-NBP2-97067-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Atlastin-3 Antibody [NBP2-69023] - Staining of human cell line SiHa shows localization to endoplasmic reticulum.](http://images.novusbio.com/fullsize/Atlastin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-69023-img0001.jpg "Immunocytochemistry/Immunofluorescence: Atlastin-3 Antibody [NBP2-69023] - Staining of human cell line SiHa shows localization to endoplasmic reticulum.")
![Western Blot: Atlastin-3 Antibody [NBP1-59035] - Human Lung lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/Atlastin-3-Antibody-Western-Blot-NBP1-59035-img0002.jpg "Western Blot: Atlastin-3 Antibody [NBP1-59035] - Human Lung lysate, concentration 0.2-1 ug/ml.")
![Western Blot: Atlastin-3 Antibody [NBP1-59034] - HCT15 cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/Atlastin-3-Antibody-Western-Blot-NBP1-59034-img0002.jpg "Western Blot: Atlastin-3 Antibody [NBP1-59034] - HCT15 cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: Atlastin-3 Overexpression Lysate (Adult Normal) [NBP2-07170] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Atlastin-3.](http://images.novusbio.com/fullsize/Atlastin-3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07170-img0001.jpg "Western Blot: Atlastin-3 Overexpression Lysate (Adult Normal) [NBP2-07170] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Atlastin-3.")