| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26527-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48188 | |||
| Azide and BSA Free | NBP2-34399-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14162 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-24312 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14096 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26526-50ul | |||
![Immunohistochemistry: Actin Antibody (25E3) [NBP3-26527] - Image of Actin Antibody (25E3) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26527_rabbit-actin-mab-25e3-262202414471559.jpg "Immunohistochemistry: Actin Antibody (25E3) [NBP3-26527] - Image of Actin Antibody (25E3) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Western Blot: Actin Antibody (25E3) [NBP3-26527] - Positive Western Blot detected in: Hela whole cell lysate, A549 whole cell lysate, Raw264.7 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, Rat brain tissue, Rat heart tissue. All lanes: Actin Antibody at 0.95ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.Predicted band size: 42 KDaObserved band size: 42 KDa](http://images.novusbio.com/fullsize/nbp3-26527_rabbit-actin-mab-25e3-282202411485845.jpg)
![Immunocytochemistry/Immunofluorescence: Actin Antibody (25E3) [NBP3-26527] - Staining of HepG2 cells with Actin Antibody (25E3) at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26527_rabbit-actin-mab-25e3-28220241323632.jpg)
![Flow Cytometry: Actin Antibody (25E3) [NBP3-26527] - Overlay histogram showing Hela cells stained with Actin Antibody (25E3) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26527_rabbit-actin-mab-25e3-282202414312731.jpg)
![Western Blot: pan Actin Antibody (A2E1-R) [NBP3-32712] - Western blot analysis of pan Actin on different lysates with Mouse anti-pan Actin antibody (NBP3-32712) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: MCF7 cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: NIH/3T3 cell lysate (20 ug/Lane) Lane 5: PC-12 cell lysate (20 ug/Lane) Lane 6: Mouse smooth muscle tissue lysate (40 ug/Lane) Lane 7: Rat heart tissue lysate (40 ug/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32712) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32712_mouse-pan-actin-mab-a2e1-r-2782024163166.jpg "Western Blot: pan Actin Antibody (A2E1-R) [NBP3-32712] - Western blot analysis of pan Actin on different lysates with Mouse anti-pan Actin antibody (NBP3-32712) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: MCF7 cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: NIH/3T3 cell lysate (20 ug/Lane) Lane 5: PC-12 cell lysate (20 ug/Lane) Lane 6: Mouse smooth muscle tissue lysate (40 ug/Lane) Lane 7: Rat heart tissue lysate (40 ug/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32712) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: pan Actin Antibody (A2E1-R) [NBP3-32712] - Immunohistochemical analysis of paraffin-embedded human heart tissue with Mouse anti-pan Actin antibody (NBP3-32712) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32712) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32712_mouse-pan-actin-mab-a2e1-r-29820241492695.jpg)
![Immunocytochemistry/ Immunofluorescence: pan Actin Antibody (A2E1-R) [NBP3-32712] - Immunocytochemistry analysis of HeLa cells labeling pan Actin with Mouse anti-pan Actin antibody (NBP3-32712) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-pan Actin antibody (NBP3-32712) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32712_mouse-pan-actin-mab-a2e1-r-29202410334034.jpg)
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (SPM160) [NBP2-34351] - Formalin-paraffin leiomyosarcoma (20X) stained with muscle specific actin MAb (SPM160)](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-SPM160-Immunohistochemistry-Paraffin-NBP2-34351-img0002.jpg "Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (SPM160) [NBP2-34351] - Formalin-paraffin leiomyosarcoma (20X) stained with muscle specific actin MAb (SPM160)")
![Immunohistochemistry-Paraffin: Actin Antibody (ACTA1/360) [NBP3-13759] - Formalin-fixed, paraffin-embedded human skeletal muscle stained with Actin antibody (ACTA1/360). Inset: PBS instead of primary antibody; secondary only negative control.](http://images.novusbio.com/fullsize/Actin-Antibody-ACTA1-360-Immunohistochemistry-Paraffin-NBP3-13759-img0001.jpg "Immunohistochemistry-Paraffin: Actin Antibody (ACTA1/360) [NBP3-13759] - Formalin-fixed, paraffin-embedded human skeletal muscle stained with Actin antibody (ACTA1/360). Inset: PBS instead of primary antibody; secondary only negative control.")
![Immunohistochemistry-Paraffin: Actin Antibody (ACTA1/360) [NBP3-13759] - Formalin-fixed, paraffin-embedded human skeletal muscle stained with Actin antibody (ACTA1/360). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. AB, 5min.](http://images.novusbio.com/fullsize/Actin-Antibody-ACTA1-360-Immunohistochemistry-Paraffin-NBP3-13759-img0002.jpg)
![Western Blot: Actin Antibody (S05-4F6) [NBP3-19993] - Western blot detection of Actin in Hela, CHO-K1, C6, Rat Brain lysates using NBP3-19993 (1:1000 diluted). Predicted band size: 42kDa. Observed band size: 42kDa.](http://images.novusbio.com/fullsize/Actin-Antibody-S05-4F6-Western-Blot-NBP3-19993-img0002.jpg "Western Blot: Actin Antibody (S05-4F6) [NBP3-19993] - Western blot detection of Actin in Hela, CHO-K1, C6, Rat Brain lysates using NBP3-19993 (1:1000 diluted). Predicted band size: 42kDa. Observed band size: 42kDa.")
![Immunohistochemistry-Paraffin: Actin Antibody (S05-4F6) [NBP3-19993] - Immunohistochemistry of Actin in paraffin-embedded Human colon cancer tissue using NBP3-19993 at dilution 1/50](http://images.novusbio.com/fullsize/Actin-Antibody-S05-4F6-Immunohistochemistry-Paraffin-NBP3-19993-img0001.jpg)
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (ACTA1/9105R) [NBP3-23827] - Formalin-fixed, paraffin-embedded human skeletal muscle stained with Actin (Muscle Specific) Antibody (ACTA1/9105R). Inset: PBS instead of primary antibody; secondary only negative control.](http://images.novusbio.com/fullsize/nbp3-23827_rabbit-actin-muscle-specific-mab-acta1-9105r-2412025874814.jpg "Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (ACTA1/9105R) [NBP3-23827] - Formalin-fixed, paraffin-embedded human skeletal muscle stained with Actin (Muscle Specific) Antibody (ACTA1/9105R). Inset: PBS instead of primary antibody; secondary only negative control.")
![Western Blot: Actin Antibody [NBP2-15256] - Sample (30 ug of whole cell lysate) A: A549 B: HeLa 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Actin-Alpha-1-Skeletal-Muscle-Antibody-Western-Blot-NBP2-15256-img0002.jpg "Western Blot: Actin Antibody [NBP2-15256] - Sample (30 ug of whole cell lysate) A: A549 B: HeLa 10% SDS PAGE; antibody diluted at 1:1000.")
![Western Blot: Actin Antibody [NBP2-15256] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Actin-Alpha-1-Skeletal-Muscle-Antibody-Western-Blot-NBP2-15256-img0001.jpg)
![Immunohistochemistry: Actin Antibody (4C1) [NBP3-26526] - Image of Actin Antibody diluted at 1:50 and staining in paraffin-embedded human skeletal muscle tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.72% DAB.](http://images.novusbio.com/fullsize/nbp3-26526_rabbit-actin-mab-4c1-262202415475615.jpg "Immunohistochemistry: Actin Antibody (4C1) [NBP3-26526] - Image of Actin Antibody diluted at 1:50 and staining in paraffin-embedded human skeletal muscle tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.72% DAB.")
![Immunohistochemistry: Actin Antibody (4C1) [NBP3-26526] - Image of Actin Antibody diluted at 1:50 and staining in paraffin-embedded human skeletal muscle tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.72% DAB.](http://images.novusbio.com/fullsize/nbp3-26526_rabbit-actin-mab-4c1-26220241525331.jpg)
![Flow Cytometry: Actin Antibody (4C1) [NBP3-26526] - Overlay Peak curve showing A549 cells stained with Actin Antibody (4C1) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26526_rabbit-actin-mab-4c1-282202414312747.jpg)
![Western Blot: Actin Antibody (9Y9L6) [NBP3-16885] - Western blot analysis of extracts of various cell lines, using Actin antibody (NBP3-16885) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Actin-Antibody-9Y9L6-Western-Blot-NBP3-16885-img0001.jpg "Western Blot: Actin Antibody (9Y9L6) [NBP3-16885] - Western blot analysis of extracts of various cell lines, using Actin antibody (NBP3-16885) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: Actin Antibody (9Y9L6) [NBP3-16885] - Western blot analysis of lysates from Arabidopsis thaliana tissue using Actin antibody (NBP3-16885). Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Actin-Antibody-9Y9L6-Western-Blot-NBP3-16885-img0002.jpg)