BAM1402) were stained with the indicated concentrations of Recombinant Human B7‑1/CD80 His-tag Alexa Fluor® 647 Protein (Catalog # AFR9050). " alt="Streptavidin coated beads conjugated to biotinylated Human B7‑1/CD80 (BAM1402) were stained with the indicated concentrations of Recombinant Human B7‑1/CD80 His-tag Alexa Fluor® 647 Protein (Catalog # AFR9050). " class="big_thumb" />
7268-CT) is immobilized at 1 µg/mL, Biotinylated Recombinant Human B7-1/CD80 His-tag Avi-tag protein (Catalog # AVI9050) binds with an ED50 of 0.1-0.6 µg/mL." alt="When Recombinant Human CTLA-4 Fc Chimera (Catalog # 7268-CT) is immobilized at 1 µg/mL, Biotinylated Recombinant Human B7-1/CD80 His-tag Avi-tag protein (Catalog # AVI9050) binds with an ED50 of 0.1-0.6 µg/mL." class="big_thumb" />
7268-CT) is immobilized at 0.25 µg/mL, Recombinant Human B7-1/CD80 Fc Chimera Avi-tag (Catalog # AVI10107) binds with an ED50 of 0.4-2 ng/mL." alt="When Recombinant Human CTLA-4 Fc Chimera (Catalog # 7268-CT) is immobilized at 0.25 µg/mL, Recombinant Human B7-1/CD80 Fc Chimera Avi-tag (Catalog # AVI10107) binds with an ED50 of 0.4-2 ng/mL." class="big_thumb" />
BAM1402) were stained with the indicated concentrations of Recombinant Human B7‑1/CD80 His-tag Alexa Fluor® 488 Protein (Catalog # AFG9050). " alt="Streptavidin coated beads conjugated to biotinylated Human B7‑1/CD80 (BAM1402) were stained with the indicated concentrations of Recombinant Human B7‑1/CD80 His-tag Alexa Fluor® 488 Protein (Catalog # AFG9050). " class="big_thumb" />
BAM1402) were stained with the indicated concentrations of Recombinant Human B7‑1/CD80 Fc-tag Alexa Fluor® 488 Protein (Catalog # AFG10133). " alt="Streptavidin coated beads conjugated to biotinylated Human B7‑1/CD80 (BAM1402) were stained with the indicated concentrations of Recombinant Human B7‑1/CD80 Fc-tag Alexa Fluor® 488 Protein (Catalog # AFG10133). " class="big_thumb" />
BAM1402) were stained with the indicated concentrations of Recombinant Human B7‑1/CD80 Fc-tag Alexa Fluor® 647 Protein (Catalog # AFR10133). " alt="Streptavidin coated beads conjugated to biotinylated Human B7‑1/CD80 (BAM1402) were stained with the indicated concentrations of Recombinant Human B7‑1/CD80 Fc-tag Alexa Fluor® 647 Protein (Catalog # AFR10133). " class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81100-0.025mg | |||
7268-CT) binds with an ED50 of 5-30 ng/mL." alt="When Recombinant Cynomolgus Monkey B7-1/CD80 (Catalog # 9244-B1) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human CTLA-4 Fc Chimera (Catalog # 7268-CT) binds with an ED50 of 5-30 ng/mL." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1214-SP | |||
![Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384] - Analysis using the PE/Cy5 conjugate of NBP1-43384. Staining of normal human peripheral blood cells with PE-Cy5 Mouse IgG1 kappa Isotype Control (open histogram) orAnti-Human CD80 (B7-1) PE-Cy5 (filled histogram).](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-2D10-4-Flow-Cytometry-NBP1-43384-img0006.jpg "Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384] - Analysis using the PE/Cy5 conjugate of NBP1-43384. Staining of normal human peripheral blood cells with PE-Cy5 Mouse IgG1 kappa Isotype Control (open histogram) orAnti-Human CD80 (B7-1) PE-Cy5 (filled histogram).")
![Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384] - Analysis using the FITC conjugate of NBP1-43384. Staining of the Daudi cell line with Mouse IgG1 kappa Isotype Control FITC (cat. 11-4714) (blue histogram) or Anti-Human CD80 (B7-1) FITC (purple histogram). Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-2D10-4-Flow-Cytometry-NBP1-43384-img0004.jpg)
![Flow Cytometry: B7-1/CD80 Antibody (2D10.4) [NBP1-43384] - Analysis using the PE conjugate of NBP1-43384. Analysis of human peripheral blood leukocytes using CD80 PE conjugage. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8 cells/test.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-2D10-4-Flow-Cytometry-NBP1-43384-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: B7-1/CD80 Antibody (IDEC-114 (Galiximab)) - Chimeric [NBP2-81100] - Immunofluorescence staining of fixed Daudi cells with B7-1/CD80 Antibody (IDEC-114 (Galiximab)) [NBP2-81100]. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of IDEC-114 (Galiximab) [NBP2-81100] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81100, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-IDEC-114-Galiximab-Chimeric-Immunocytochemistry-Immunofluorescence-NBP2-81100-img0001.jpg "Immunocytochemistry/Immunofluorescence: B7-1/CD80 Antibody (IDEC-114 (Galiximab)) - Chimeric [NBP2-81100] - Immunofluorescence staining of fixed Daudi cells with B7-1/CD80 Antibody (IDEC-114 (Galiximab)) [NBP2-81100]. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of IDEC-114 (Galiximab) [NBP2-81100] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81100, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.")
![N/A B7-1/CD80 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_B71_DY140_ELISA_1642.jpg "N/A B7-1/CD80 [Biotin]")
