| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-43670-25ul | |||
| Azide and BSA Free | NBP3-44278 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-43670-100ul | |||
| BSA Free | NBP3-43670-25ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NBP1-28379 | |||
| Biotin | NBP1-28377 | |||
| PE | NBP1-28378 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7489R-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB11516-SP | |||
![Western Blot: CD19 Antibody (BLR137H) [NBP3-14717] - Whole cell lysate (10 ug) from Ramos, HeLa, HEK293T, Raji, Hep-G2, MCF-7, Jurkat, and Daudi cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD19 recombinant monoclonal antibody [BLR137H] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.](http://images.novusbio.com/fullsize/CD19-Antibody-BLR137H-Western-Blot-NBP3-14717-img0001.jpg "Western Blot: CD19 Antibody (BLR137H) [NBP3-14717] - Whole cell lysate (10 ug) from Ramos, HeLa, HEK293T, Raji, Hep-G2, MCF-7, Jurkat, and Daudi cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD19 recombinant monoclonal antibody [BLR137H] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.")
![Immunocytochemistry/Immunofluorescence: CD19 Antibody (BLR137H) [NBP3-14717] - Rabbit anti-CD19 recombinant monoclonal antibody [BLR137H. ]Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CD19-Antibody-BLR137H-Immunocytochemistry-Immunofluorescence-NBP3-14717-img0003.jpg)
![Immunohistochemistry-Paraffin: CD19 Antibody (BLR137H) [NBP3-14717] - Rabbit anti-CD19 recombinant monoclonal antibody [BLR137H]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CD19-Antibody-BLR137H-Immunohistochemistry-Paraffin-NBP3-14717-img0002.jpg)
![Immunohistochemistry-Frozen: CD19 Antibody (SJ25-C1) [NBP1-28375] - CD19 (red) stained in human skin, counterstained with DAPI (blue) at 20x. Tissue fixed with 4% PFA, permeabilized with 0.5% Triton, and blocked with 37% Egg DI, 5% Milk DI, and 3% FSG 2% BSA TBS Ca/Azide in that order. Anti-CD19 applied at 1:500 v/v, 4C, O/N. Alexa Fluor 568 secondary antibody, with DAPI counterstain. Image from verified customer review.](http://images.novusbio.com/fullsize/CD19-Antibody-SJ25-C1-Immunohistochemistry-Frozen-NBP1-28375-img0006.jpg "Immunohistochemistry-Frozen: CD19 Antibody (SJ25-C1) [NBP1-28375] - CD19 (red) stained in human skin, counterstained with DAPI (blue) at 20x. Tissue fixed with 4% PFA, permeabilized with 0.5% Triton, and blocked with 37% Egg DI, 5% Milk DI, and 3% FSG 2% BSA TBS Ca/Azide in that order. Anti-CD19 applied at 1:500 v/v, 4C, O/N. Alexa Fluor 568 secondary antibody, with DAPI counterstain. Image from verified customer review.")
![Flow Cytometry: CD19 Antibody (SJ25-C1) [NBP1-28375] - Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19-APC and Mouse Anti-Human CD3-FITC.](http://images.novusbio.com/fullsize/CD19-Antibody-SJ25-C1-Flow-Cytometry-NBP1-28375-img0007.jpg)
![Immunoprecipitation: CD19 Antibody (PSH03-94) [NBP3-32130] - CD19 was immunoprecipitated in 0.2mg Mouse spleen tissue lysate with NBP3-32130 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32130 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse spleen tissue lysate (input) Lane 2: NBP3-32130 IP in Mouse spleen tissue lysate Lane 3: Rabbit IgG instead of NBP3-32130 in Mouse spleen tissue lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 17 seconds](http://images.novusbio.com/fullsize/nbp3-32130_rabbit-cd19-mab-psh03-94-2682024158261.jpg "Immunoprecipitation: CD19 Antibody (PSH03-94) [NBP3-32130] - CD19 was immunoprecipitated in 0.2mg Mouse spleen tissue lysate with NBP3-32130 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32130 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse spleen tissue lysate (input) Lane 2: NBP3-32130 IP in Mouse spleen tissue lysate Lane 3: Rabbit IgG instead of NBP3-32130 in Mouse spleen tissue lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 17 seconds")
![Western Blot: CD19 Antibody (PSH03-94) [NBP3-32130] - Western blot analysis of CD19 on mouse spleen tissue lysates with Rabbit anti-CD19 antibody (NBP3-32130) at 1/1,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 60 kDa Observed band size: 60-120 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32130) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32130_rabbit-cd19-mab-psh03-94-278202415212455.jpg)
![Immunohistochemistry: CD19 Antibody (PSH03-94) [NBP3-32130] - Immunofluorescence analysis of paraffin-embedded mouse spleen tissue labeling CD19 with Rabbit anti-CD19 antibody (NBP3-32130) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32130, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32130_rabbit-cd19-mab-psh03-94-29820241420976.jpg)
![Immunohistochemistry: CD19 Antibody (PSH03-94) [NBP3-32130] - Immunohistochemical analysis of paraffin-embedded mouse lymph node tissue with Rabbit anti-CD19 antibody (NBP3-32130) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32130) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32130_rabbit-cd19-mab-psh03-94-2982024149261.jpg)
