Products Related to Gene-ID: 930

HAF008). A specific band was detected for CD19 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF008). A specific band was detected for CD19 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western Blot shows lysates of Raji human Burkitt's lymphoma cell line and NC‑37 human Burkitt's lymphoma B lymphoblast cell line. PVDF membrane was probed with 2 µg/ml of Rabbit Anti-Human CD19 Monoclonal Antibody (Catalog # MAB11516) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for CD19 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." alt="CD19 was detected in immersion fixed frozen sections of rat spleen using Mouse Anti-Rat CD19 Monoclonal Antibody (Catalog # MAB7489R) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

FC001) then stained with Human CD4 PE-Cy7-conjugated Antibody and rhCD19 Atto 647N-conjugated protein (ATM9269) for 25 min at room temp in the dark. Staining was performed using our staining membrane proteins protocol." class="big_lightbox"> FC001) then stained with Human CD4 PE-Cy7-conjugated Antibody and rhCD19 Atto 647N-conjugated protein (ATM9269) for 25 min at room temp in the dark. Staining was performed using our staining membrane proteins protocol." alt="Human PBMC CD4+ and CD8+ T cells were either (A) transduced or (B) not transduced (negative control) with a Human CD19 CAR and then cultured for 11 days. Cells were washed twice with 2mL 1x PBS then stained with a viability dye for 15-30 min at room temp in the dark. Human block (1-001-1) was added during the viability stain to block Fc receptors. Cells were washed twice with Flow Cytometry Staining Buffer (FC001) then stained with Human CD4 PE-Cy7-conjugated Antibody and rhCD19 Atto 647N-conjugated protein (ATM9269) for 25 min at room temp in the dark. Staining was performed using our staining membrane proteins protocol." class="big_thumb" />

ATM9269) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 77-92 kDa and 154-184 kDa, respectively." class="big_lightbox"> ATM9269) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 77-92 kDa and 154-184 kDa, respectively." class="big_thumb" />