MAB002), followed by PE-conjugated anti-Mouse IgG secondary antibody (F0102B) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (FAB100A). Staining was performed using our Staining Membrane-associated Proteins protocol. " alt="Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # MAB37913) or (B) Mouse IgG1 Isotype Control (MAB002), followed by PE-conjugated anti-Mouse IgG secondary antibody (F0102B) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (FAB100A). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB37913-SP | |||
| Carrier Free | FAB37913T-100UG | |||
| Carrier Free | FAB37913V-100UG | |||
| Carrier Free | FAB37913G-100UG | |||
| Carrier Free | FAB37913U-100UG | |||
| Carrier Free | FAB37913R-100UG | |||
| Carrier Free | FAB37913S-100UG | |||
| Carrier Free | FAB37913N-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75910SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52670-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26682-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20762 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11421 | |||
![Immunohistochemistry: CD4 Antibody (CE9.1 (Clenoliximab)) [NBP2-75910] - Anti-CD4 staining of formaldehyde fixed paraffin embedded guinea pig skin tissue, at 40x magnification. The human IgG4-chimeric version of CE9.1 (Clenoliximab) (NBP2-75910) was used to stain samples at a concentration of 3 ug/ml.](http://images.novusbio.com/fullsize/CD4-Antibody-CE9-1-Clenoliximab-Immunohistochemistry-NBP2-75910-img0002.jpg "Immunohistochemistry: CD4 Antibody (CE9.1 (Clenoliximab)) [NBP2-75910] - Anti-CD4 staining of formaldehyde fixed paraffin embedded guinea pig skin tissue, at 40x magnification. The human IgG4-chimeric version of CE9.1 (Clenoliximab) (NBP2-75910) was used to stain samples at a concentration of 3 ug/ml.")
![Western Blot: CD4 Antibody (CE9.1 (Clenoliximab)) [NBP2-75910] - Human thyroid tumour samples were resolved on a 15% SDS PAGE gel and blots probed with the human IgG4-chimeric version of CE9.1 (NBP2-75910) at 1.5 ug/ml before detection using an anti-human IgG4 secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for CD4 is 51kDa (arrowhead). Here some non-specific bands can also be seen. NBP2-75910 successfully detected CD4 in human thyroid tissue samples.](http://images.novusbio.com/fullsize/CD4-Antibody-CE9-1-Clenoliximab-Western-Blot-NBP2-75910-img0003.jpg)
![Flow Cytometry: CD4 Antibody (13B8.2) - Chimeric [NBP2-52670] - Flow-cytometry using anti-CD4 antibody 13B8.2. Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of 13B8.2 (NBP2-52670, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD4-Antibody-13B8-2-Chimeric-Flow-Cytometry-NBP2-52670-img0001.jpg "Flow Cytometry: CD4 Antibody (13B8.2) - Chimeric [NBP2-52670] - Flow-cytometry using anti-CD4 antibody 13B8.2. Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of 13B8.2 (NBP2-52670, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![Immunohistochemistry: CD4 Antibody (2B7) [NBP3-26682] - Image of CD4 Antibody (2B7) diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26682_rabbit-cd4-mab-2b7-262202414225214.jpg "Immunohistochemistry: CD4 Antibody (2B7) [NBP3-26682] - Image of CD4 Antibody (2B7) diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Immunohistochemistry: CD4 Antibody (2B7) [NBP3-26682] - Image of CD4 Antibody (2B7) diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26682_rabbit-cd4-mab-2b7-262202415253330.jpg)
![Western Blot: CD4 Antibody (2B7) [NBP3-26682] - Positive Western Blot detected in Jurkat whole cell lysate.<br /> All lanes: CD4 Antibody at 0.8ug/ml.<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 55 KDa<br /> Observed band size: 55 KDa<br />](http://images.novusbio.com/fullsize/nbp3-26682_rabbit-cd4-mab-2b7-28220241152515.jpg)
