Cyclin E1: Novus Biologicals

AF6810

Cyclin E1 Antibody [Unconjugated]

(1 Publication)

100 μg

AF6810

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and HEK293 human embryonic kidney cell line. Gels were loaded with 25 µg of cytoplasmic (Cyto) and 25 µg of nuclear (Nuc) extracts. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Cyclin E1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6810) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). Specific bands were detected for Cyclin E1 at approximately 50 - 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Cyclin E1 was detected in immersion fixed paraffin-embedded sections of human breast using Sheep Anti-Human Cyclin E1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6810) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Cyclin E1 at approximately 53 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Cyclin E1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6810) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.

Activation of Sox17 at onset of EC regeneration and Sox17-mediated Cyclin E1 expression. a qPCR analysis of gene expression in sorted CD31+ cells from mTmG-Scl mice before and after injury (12 mg/kg i.p.). Sox17, Vegfr2, and Ccne1 increased significantly at day 2 post-LPS compared to baseline. n = 3. Color scale: the fold change increases from red to white to green color. b Western blot analysis in fresh isolated ECs from wild-type mice and quantification c showed a 5-fold increase in Sox17 protein expression within 1 day following injury compared to baseline and followed by recovery within 3 days post-LPS. n = 3. d, e Western blot analysis of cultured HLMVECs in which Sox17 was overexpressed showed 2.5x fold increase in Cyclin E1 protein expression relative to control cells. n = 3. OE, overexpression. f Representation of the CCNE1 promoter region with Sox17 binding sites (circled numbers) and their sequences. g HLMVECs were retrovirally transduced with Sox17 or control plasmid for 3 days, and Ch-IP assay followed by qPCR was performed to amplify Sox17 binding sites in the CCNE1 promoter. n = 3. h 293T cells were transfected with a Sox17 overexpression plasmid containing CCNE1 luciferase reporter constructs. Luciferase values were normalized to Renilla luciferase control reporter values. A schematic representation of corresponding deletion constructs is presented in the right panel. n = 3 and duplicates per sample. **P < 0.01 and ***P < 0.001. Data are shown as mean ± SEM. Analysis was performed using one-way ANOVA for (c) and two-way ANOVA with Bonferroni post-tests for (e, g, h) Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31073164), licensed under a CC-BY license. Not internally tested by R&D Systems.

$Overexpression of Sox17 in ECs induces EC proliferation and regeneration. Mixture of 50 μg plasmid with 100 μl liposomes was injected i.v. 3 h after LPS challenge (12 mg/dose i.p.) in wild-type mice. This plasmid has a Flag-tag added to the N-terminus of Sox17 protein coding region and expression is under the regulation of a mouse Cdh5 promoter. a Confocal microscopy of flag staining with CD31 and DAPI co-staining for nuclei in lung cryo-sections from mice receiving a control vector or a Sox17-construct to over-express Sox17. Scale bar = 50 μm (original panel) and 20 μm (enlarged panel). n = 6. OE, overexpression. b Co-localization coefficient for the fraction of Flag in CD31+ cells assesses the transgene expression in the endothelium. The Pearson correlation coefficient is significantly increased in Sox17-overexpressing mice compared to control mice. n = 6. c Western blot analysis and its quantification d showed a significant increase in the flag and Cyclin E1 expression in the pulmonary endothelial cells of mice with 3 days of Sox17 overexpression compared to vector mice. n = 3. e Quantification of BrdU+ nuclei in each field of 425 μm2 area in lung cryo-sections from vector-overexpressing and Sox17-overexpressing mice. n = 5 per group and 6 technical replicates per sample. Slides are co-stained with CD31-AF594, BrdU-AF488, and DAPI. Both groups show increased BrdU+ ECs at day 3 post-LPS as compared to baseline and the response was significantly greater in mice in which ECs overexpressed Sox 17. f Lung transvascular albumin permeability pre-LPS and post-LPS challenge in mice overexpressing endothelial Sox17 and control mice. n = 5. Mice overexpressing Sox17 in ECs showed significantly reduced vascular leakiness post-LPS when compared to control mice. g Survival curve of LPS challenge in control mice and mice over-expressing Sox17 in the endothelium. n = 11 per group. At this lethal dose of LPS (20 mg/kg), the death rate for control mice is 60% while for Sox17-overexpressed mice is 10%. h Model. LPS induces tissue hypoxia due to local oxygen depletion by infiltrating activated neutrophils, thereby stabilizing HIF-1 alpha resulting in upregulation Sox17 expression and Sox17 mediated expression of Cyclin E1. This activates cell cycle re-entry and EC proliferation, and restoration of endothelial integrity. **P < 0.01 and ***P < 0.001. Data are shown as mean ± SEM. Analysis was performed using two-way ANOVA with Bonferroni post-tests for (d–f) and Log-rank (Mantel-Cox) test for (g) Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31073164), licensed under a CC-BY license. Not internally tested by R&D Systems.$

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF6810U-100UG
Alexa Fluor 405	AF6810V-100UG
Alexa Fluor 488	AF6810G-100UG
Alexa Fluor 532	AF6810X-100UG
Alexa Fluor 594	AF6810T-100UG
Alexa Fluor 647	AF6810R-100UG
Alexa Fluor 700	AF6810N-100UG
Alexa Fluor 750	AF6810S-100UG
Allophycocyanin	AF6810A
Biotin	AF6810B
CoraFluor 1	AF6810CL1
DyLight 350	AF6810D
DyLight 405	AF6810E
DyLight 488	AF6810K
DyLight 550	AF6810L
DyLight 594	AF6810M
DyLight 650	AF6810W
DyLight 680	AF6810Y
DyLight 755	AF6810Z
FITC	AF6810F
HRP	AF6810H
Janelia Fluor 525	AF6810JF525
Janelia Fluor 549	AF6810I
Janelia Fluor 646	AF6810J
Janelia Fluor 669	AF6810JF669
PE	AF6810P
PerCP	AF6810C
mFluor Violet 450 SE	AF6810MFV450
mFluor Violet 500 SE	AF6810MFV500
mFluor Violet 610 SE	AF6810MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF6810-SP

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NBP1-88153

Cyclin E1 Antibody - BSA Free

(1 Publication)

0.1 ml

NBP1-88153

Staining of human cerebral cortex shows strong nuclear positivity in neurons.

Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Staining of human liver shows no nuclear positivity in hepatocytes as expected.

Staining of human placenta shows strong nuclear positivity in decidual cells.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-88153-25ul

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NBP3-05378

Cyclin E1 [p Thr395] Antibody - BSA Free

100 ul

NBP3-05378

Immunocytochemistry/ Immunofluorescence: Cyclin E1 [p Thr395] Antibody - BSA Free [NBP3-05378] - Immunofluorescence analysis of U2OS cells using Cyclin E1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: Cyclin E1 [p Thr395] Antibody - BSA Free [NBP3-05378] - Immunofluorescence analysis of NIH/3T3 cells using Cyclin E1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-05378-20ul

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NBP2-16055

Cyclin E1 Antibody

0.1 ml

NBP2-16055

<b>Biological Strategies Validation. </b>Western Blot: Cyclin E1 Antibody [NBP2-16055] - Untreated (-) and treated (+) HCT-116 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin E1 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Cyclin E1 Antibody [NBP2-16055] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cyclin E1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Reactivity:

Hu, Pm

Species Glossary

Reactivity: Hu, Pm

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NB500-129

Cyclin E1 Antibody - BSA Free

0.1 mg

NB500-129

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-4020

Alt Catalog# IMG-4020

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB500-129AF350
Alexa Fluor 405	NB500-129AF405
Alexa Fluor 488	NB500-129AF488
Alexa Fluor 532	NB500-129AF532
Alexa Fluor 594	NB500-129AF594
Alexa Fluor 647	NB500-129AF647
Alexa Fluor 700	NB500-129AF700
Alexa Fluor 750	NB500-129AF750
Allophycocyanin	NB500-129APC
Biotin	NB500-129B
CoraFluor 1	NB500-129CL1
DyLight 350	NB500-129UV
DyLight 405	NB500-129V
DyLight 488	NB500-129G
DyLight 550	NB500-129R
DyLight 594	NB500-129DL594
DyLight 650	NB500-129C
DyLight 680	NB500-129FR
DyLight 755	NB500-129IR
FITC	NB500-129F
HRP	NB500-129H
Janelia Fluor 525	NB500-129JF525
Janelia Fluor 549	NB500-129JF549
Janelia Fluor 646	NB500-129JF646
Janelia Fluor 669	NB500-129JF669
PE	NB500-129PE
PerCP	NB500-129PCP
mFluor Violet 450 SE	NB500-129MFV450
mFluor Violet 500 SE	NB500-129MFV500
mFluor Violet 610 SE	NB500-129MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB500-129SS

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AF6810X

Cyclin E1 Antibody [Alexa Fluor® 532]

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Conjugate:

Alexa Fluor 532

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AF6810T

Cyclin E1 Antibody [Alexa Fluor® 594]

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Conjugate:

Alexa Fluor 594

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AF6810R

Cyclin E1 Antibody [Alexa Fluor® 647]

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Conjugate:

Alexa Fluor 647

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AF6810G

Cyclin E1 Antibody [Alexa Fluor® 488]

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Conjugate:

Alexa Fluor 488

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AF6810N

Cyclin E1 Antibody [Alexa Fluor® 700]

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Conjugate:

Alexa Fluor 700

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AF6810V

Cyclin E1 Antibody [Alexa Fluor® 405]

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Conjugate:

Alexa Fluor 405

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AF6810U

Cyclin E1 Antibody [Alexa Fluor® 350]

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Conjugate:

Alexa Fluor 350

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Host

Available Conjugates

Format

Modification

Clonality

Images Type

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Products Related to Gene-ID: 898

Cyclin E1 Antibody [Unconjugated]

Cyclin E1 Antibody - BSA Free

Cyclin E1 [p Thr395] Antibody - BSA Free

Cyclin E1 Antibody

Cyclin E1 Antibody - BSA Free

Cyclin E1 Antibody [Alexa Fluor® 532]

Cyclin E1 Antibody [Alexa Fluor® 594]

Cyclin E1 Antibody [Alexa Fluor® 647]

Cyclin E1 Antibody [Alexa Fluor® 488]

Cyclin E1 Antibody [Alexa Fluor® 700]

Cyclin E1 Antibody [Alexa Fluor® 405]

Cyclin E1 Antibody [Alexa Fluor® 350]