| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47610-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41171-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-42189-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-46768-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41171 | |||
| BSA Free | NBP2-41171-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41171 | |||
| BSA Free | NBP2-41171-0.025mg | |||
![Western Blot: SHANK3 Antibody (S69) [NBP1-47610] - Western Blot analysis of Rat brain membrane lysate showing detection of SHANK3 protein using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46 (NBP1-47610). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody (NBP1-47610) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/SHANK3-Antibody-S69-Western-Blot-NBP1-47610-img0005.jpg "Western Blot: SHANK3 Antibody (S69) [NBP1-47610] - Western Blot analysis of Rat brain membrane lysate showing detection of SHANK3 protein using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46 (NBP1-47610). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody (NBP1-47610) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: SHANK3 Antibody (S69) [NBP1-47610] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46 (NBP1-47610). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody (NBP1-47610) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK3 Antibody (D) Composite.](http://images.novusbio.com/fullsize/SHANK3-Antibody-S69-Immunocytochemistry-Immunofluorescence-NBP1-47610-img0007.jpg)
![Immunohistochemistry: SHANK3 Antibody (S69) [NBP1-47610] - Immunohistochemistry analysis using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46 (NBP1-47610). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody (NBP1-47610) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Early stages of filaggrin-like and dermal staining.](http://images.novusbio.com/fullsize/SHANK3-Antibody-S69-Immunohistochemistry-NBP1-47610-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: SHANK3 Antibody (S69) [NBP1-47610] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Borderline positive.](http://images.novusbio.com/fullsize/SHANK3-Antibody-S69-Immunocytochemistry-Immunofluorescence-NBP1-47610-img0003.jpg)
![Western Blot: SHANK3 Antibody [NBP2-41171] - Western blot analysis of SHANK3 in 3T3 cell lysate with SHANK3 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/SHANK3-Antibody-Western-Blot-NBP2-41171-img0001.jpg "Western Blot: SHANK3 Antibody [NBP2-41171] - Western blot analysis of SHANK3 in 3T3 cell lysate with SHANK3 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.")
![Immunohistochemistry: SHANK3 Antibody - BSA Free [NBP2-41171] - Immunohistochemistry of SHANK3 in human brain tissue with SHANK3 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41171_rabbit-polyclonal-shank3-antibody-2711202420333840.jpg)
![Immunocytochemistry/ Immunofluorescence: SHANK3 Antibody - BSA Free [NBP2-41171] - Immunofluorescence of SHANK3 in human brain tissue with SHANK3 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41171_rabbit-polyclonal-shank3-antibody-271120241926181.jpg)
![Western Blot: SHANK3 Antibody (S367-51) [NBP2-42189] - Western Blot analysis of Monkey COS cells transfected with HA-tagged Shank1 showing detection of ~190 kDa SHANK1/SHANK3 protein using Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody, Clone S367-51 (NBP2-42189). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with HA-tagged Shank1. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody (NBP2-42189) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~190 kDa.](http://images.novusbio.com/fullsize/SHANK3-Antibody-S367-51-Western-Blot-NBP2-42189-img0003.jpg "Western Blot: SHANK3 Antibody (S367-51) [NBP2-42189] - Western Blot analysis of Monkey COS cells transfected with HA-tagged Shank1 showing detection of ~190 kDa SHANK1/SHANK3 protein using Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody, Clone S367-51 (NBP2-42189). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with HA-tagged Shank1. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody (NBP2-42189) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~190 kDa.")
![Immunocytochemistry/Immunofluorescence: SHANK3 Antibody (S367-51) [NBP2-42189] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody, Clone S367-51 (NBP2-42189). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody (NBP2-42189) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK1/SHANK3 Antibody (D) Composite.](http://images.novusbio.com/fullsize/SHANK3-Antibody-S367-51-Immunocytochemistry-Immunofluorescence-NBP2-42189-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SHANK3 Antibody (S367-51) [NBP2-42189] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/SHANK3-Antibody-S367-51-Immunocytochemistry-Immunofluorescence-NBP2-42189-img0002.jpg)
![Western Blot: SHANK3 Antibody [NBP3-13162] - Various tissue extracts (75 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Shank3 antibody (NBP3-13162) diluted at 1:1000.](http://images.novusbio.com/fullsize/SHANK3-Antibody-Western-Blot-NBP3-13162-img0001.jpg "Western Blot: SHANK3 Antibody [NBP3-13162] - Various tissue extracts (75 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Shank3 antibody (NBP3-13162) diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: SHANK3 Antibody [NBP3-13162] - SHANK3 antibody detects SHANK3 protein at cell body and synaptic vesicles by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: SHANK3 protein stained by SHANK3 antibody (NBP3-13162) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/SHANK3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13162-img0002.jpg)
![Immunohistochemistry-Frozen: SHANK3 Antibody [NBP3-13162] - SHANK3 antibody detects SHANK3 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: SHANK3 protein stained by SHANK3 antibody (NBP3-13162) diluted at 1:250. Red: NeuN, stained by NeuN antibody [2Q158] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/SHANK3-Antibody-Immunohistochemistry-Frozen-NBP3-13162-img0003.jpg)
![Immunohistochemistry-Paraffin: SHANK3 Antibody [NBP1-82900] - Staining of human placenta shows strong positivity in endothelial cells.](http://images.novusbio.com/fullsize/SHANK3-Antibody-Immunohistochemistry-Paraffin-NBP1-82900-img0003.jpg "Immunohistochemistry-Paraffin: SHANK3 Antibody [NBP1-82900] - Staining of human placenta shows strong positivity in endothelial cells.")
![Immunohistochemistry-Paraffin: SHANK3 Antibody [NBP1-82900] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/SHANK3-Antibody-Immunohistochemistry-Paraffin-NBP1-82900-img0001.jpg)
![Immunohistochemistry-Paraffin: SHANK3 Antibody [NBP1-82900] - Staining of human cerebral cortex shows moderate positivity in neuropil.](http://images.novusbio.com/fullsize/SHANK3-Antibody-Immunohistochemistry-Paraffin-NBP1-82900-img0002.jpg)
![Immunohistochemistry-Paraffin: SHANK3 Antibody [NBP1-82900] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/SHANK3-Antibody-Immunohistochemistry-Paraffin-NBP1-82900-img0004.jpg)
![Western Blot: SHANK3 Antibody [NBP3-13167] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SHANK3 antibody (NBP3-13167) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SHANK3-Antibody-Western-Blot-NBP3-13167-img0001.jpg "Western Blot: SHANK3 Antibody [NBP3-13167] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SHANK3 antibody (NBP3-13167) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: SHANK3 Antibody [NBP3-13167] - SHANK3 antibody detects SHANK3 protein by immunofluorescent analysis. Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: SHANK3 protein stained by SHANK3 antibody (NBP3-13167) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/SHANK3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13167-img0002.jpg)
![Western Blot: SHANK3 Antibody [NBP2-32161] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-SHANK3 antibody NBP2-32161 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/SHANK3-Antibody-Western-Blot-NBP2-32161-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: SHANK3 Antibody [NBP2-32161] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-SHANK3 antibody NBP2-32161 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: SHANK3 Antibody [NBP2-32161] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SHANK3 antibody NBP2-32161 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated SHANK3, NBP2-32161 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/SHANK3-Antibody-Immunoprecipitation-NBP2-32161-img0002.jpg)