NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="Caspase-8 was detected in immersion fixed Jurkat human acute T cell leukemia cell line treated for 4 hours with staurosporin using Mouse Anti-Human Caspase-8 Polyclonal Antibody (Catalog # MAB8135) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | mab8135-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88185-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88184-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58881-25ul | |||
![Western Blot: Caspase-8 Antibody (1G12) - BSA Free [NBP2-80097] - Using Caspase-8 (mouse), mAb (1G12) (Prod. No. NBP2-80097) detecting the cleaved active p20 subunit of mouse caspase-8 in addition to the caspase-8 precursor, upon an apoptotic stimulus e.g. by cross-linked rhsFasL.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-1G12-BSA-Free-Western-Blot-NBP2-80097-img0004.jpg "Western Blot: Caspase-8 Antibody (1G12) - BSA Free [NBP2-80097] - Using Caspase-8 (mouse), mAb (1G12) (Prod. No. NBP2-80097) detecting the cleaved active p20 subunit of mouse caspase-8 in addition to the caspase-8 precursor, upon an apoptotic stimulus e.g. by cross-linked rhsFasL.")
![Flow Cytometry: Caspase-8 Antibody (1G12) - BSA Free [NBP2-80097] - Data in permeabilized cells of overexpressed FLAG-epitope-tagged pro-caspase-8 (active-site-mutant) in 293T cells using Caspase-8 mAbs (1G12) (Prod. No. NBP2-80097)](http://images.novusbio.com/fullsize/Caspase-8-Antibody-1G12-BSA-Free-Flow-Cytometry-NBP2-80097-img0001.jpg)
![Western Blot: Caspase-8 Antibody (1G12) - BSA Free [NBP2-80097] - Using Caspase-8 (mouse), mAb (1G12) (Prod. No. NBP2-80097) detecting endogenous caspase-8 in various mouse cell line, but not in human cell lines.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-1G12-BSA-Free-Western-Blot-NBP2-80097-img0002.jpg)
![Western Blot: Caspase-8 Antibody (1G12) - BSA Free [NBP2-80097] - Using Caspase-8 (mouse), mAb (1G12) (Prod. No. NBP2-80097) detecting endogenous caspase-8 in MEFs from WT mice, but not in MEFs from caspase-8-/- mice.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-1G12-BSA-Free-Western-Blot-NBP2-80097-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Caspase-8 Antibody [NBP1-88185] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88185-img0007.jpg "Immunocytochemistry/Immunofluorescence: Caspase-8 Antibody [NBP1-88185] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol.")
![Immunohistochemistry-Paraffin: Caspase-8 Antibody [NBP1-88185] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center and non-germinal center cells.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Immunohistochemistry-Paraffin-NBP1-88185-img0008.jpg)
![Immunohistochemistry-Paraffin: Caspase-8 Antibody [NB600-576] - Formalin fixed paraffin embedded human stomach stained with Caspase-8 antibody (NB600-576).](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Immunohistochemistry-Paraffin-NB600-576-img0002.jpg "Immunohistochemistry-Paraffin: Caspase-8 Antibody [NB600-576] - Formalin fixed paraffin embedded human stomach stained with Caspase-8 antibody (NB600-576).")
![Immunohistochemistry: Caspase-8 Antibody [NB600-576] - Formalin-fixed, paraffin-embedded human stomach stained with Caspase 8. Note cytoplasmic staining of epithelial cells.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Immunohistochemistry-NB600-576-img0001.jpg)
![Immunohistochemistry-Paraffin: Caspase-8 Antibody [NBP1-88184] - Staining of human tonsil shows strong cytoplasmic positivity in germinal center cells and non-germinal center cells.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Immunohistochemistry-Paraffin-NBP1-88184-img0006.jpg "Immunohistochemistry-Paraffin: Caspase-8 Antibody [NBP1-88184] - Staining of human tonsil shows strong cytoplasmic positivity in germinal center cells and non-germinal center cells.")
![Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17<br/>Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/nbp1-88184_rabbit-polyclonal-caspase-8-antibody-65202516162417.jpg)
![ELISA: Rat Caspase-8 ELISA Kit (Colorimetric) [NBP2-75041] - Samples were spiked with high concentrations of Rat Caspase-8 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75041_rat-caspase-8-elisa-kit-colorimetric-132202416175611.jpg "ELISA: Rat Caspase-8 ELISA Kit (Colorimetric) [NBP2-75041] - Samples were spiked with high concentrations of Rat Caspase-8 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Caspase-8 ELISA Kit (Colorimetric) [NBP2-75041] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75041_rat-caspase-8-elisa-kit-colorimetric-432024983565.jpg)
![ELISA: Mouse Caspase-8 ELISA Kit (Colorimetric) [NBP2-75040] - Samples were spiked with high concentrations of Mouse Caspase-8 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75040_mouse-caspase-8-elisa-kit-colorimetric-132202416235738.jpg "ELISA: Mouse Caspase-8 ELISA Kit (Colorimetric) [NBP2-75040] - Samples were spiked with high concentrations of Mouse Caspase-8 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Caspase-8 ELISA Kit (Colorimetric) [NBP2-75040] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75040_mouse-caspase-8-elisa-kit-colorimetric-432024915869.jpg)
![Western Blot: Caspase 8 Overexpression Lysate (Adult Normal) [NBP2-10964] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Caspase 8.](http://images.novusbio.com/fullsize/Caspase-8-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10964-img0001.jpg "Western Blot: Caspase 8 Overexpression Lysate (Adult Normal) [NBP2-10964] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Caspase 8.")
![Western Blot: Caspase 8 Overexpression Lysate (Adult Normal) [NBL1-08717] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Caspase 8.](http://images.novusbio.com/fullsize/Caspase-8-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-08717-img0002.jpg "Western Blot: Caspase 8 Overexpression Lysate (Adult Normal) [NBL1-08717] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Caspase 8.")
