| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92924-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58494-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-56453-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22169-50ul | |||
![Western Blot: CAD Antibody [NBP2-92924] - Analysis of extracts of various cell lines, using CAD at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/CAD-Antibody-Western-Blot-NBP2-92924-img0009.jpg "Western Blot: CAD Antibody [NBP2-92924] - Analysis of extracts of various cell lines, using CAD at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")
![Immunohistochemistry-Paraffin: CAD Antibody [NBP2-92924] - Rat testis using CAD antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/CAD-Antibody-Immunohistochemistry-Paraffin-NBP2-92924-img0008.jpg)
![Immunohistochemistry-Paraffin: CAD Antibody [NBP2-92924] - Human tonsil using CAD antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/CAD-Antibody-Immunohistochemistry-Paraffin-NBP2-92924-img0006.jpg)
![Immunohistochemistry-Paraffin: CAD Antibody [NBP2-92924] - Mouse spleen using CAD antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/CAD-Antibody-Immunohistochemistry-Paraffin-NBP2-92924-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CAD Antibody [NBP2-92924] - Analysis of NIH-3T3 cells using CAD at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CAD-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92924-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: CAD Antibody - BSA Free [NBP2-92924] - Immunofluorescence analysis of U-2 OS cells using CAD Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92924_rabbit-polyclonal-cad-antibody-4220251429027.jpg)
![Western Blot: CAD Antibody [NB100-61614] - Whole cell lysate (50 ug) from HEK293T, K-562, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CAD antibody NB100-61614 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/CAD-Antibody-Western-Blot-NB100-61614-img0009.jpg "Western Blot: CAD Antibody [NB100-61614] - Whole cell lysate (50 ug) from HEK293T, K-562, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CAD antibody NB100-61614 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry-Paraffin: CAD Antibody [NB100-61614] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CAD used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CAD-Antibody-Immunohistochemistry-NB100-61614-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CAD Antibody [NB100-61614] - Detection of human CAD by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CAD antibody NB100-61614 (lot NB100-61614-3) used for IP at 6 ug per reaction. CAD was also immunoprecipitated by a previous lot of this antibody (lot NB100-61614-2) and another rabbit anti-CAD antibody. For blotting immunoprecipitated CAD, NB100-61614 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/CAD-Antibody-Immunoprecipitation-NB100-61614-img0010.jpg)
![Western Blot: CAD Antibody (0O8D6) [NBP3-16650] - Western blot analysis of extracts of 293T cells, using CAD antibody (NBP3-16650) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/CAD-Antibody-0O8D6-Western-Blot-NBP3-16650-img0003.jpg "Western Blot: CAD Antibody (0O8D6) [NBP3-16650] - Western blot analysis of extracts of 293T cells, using CAD antibody (NBP3-16650) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunocytochemistry/Immunofluorescence: CAD Antibody (0O8D6) [NBP3-16650] - Immunofluorescence analysis of U-2 OS cells using CAD Rabbit mAb (NBP3-16650) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CAD-Antibody-0O8D6-Immunocytochemistry-Immunofluorescence-NBP3-16650-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CAD Antibody (0O8D6) [NBP3-16650] - Immunofluorescence analysis of NIH-3T3 cells using CAD Rabbit mAb (NBP3-16650) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CAD-Antibody-0O8D6-Immunocytochemistry-Immunofluorescence-NBP3-16650-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: CAD Antibody (0O8D6) [NBP3-16650] - Confocal imaging of HeLa cells using CAD Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16650_rabbit-cad-mab-0o8d6-822025208484.jpg)
![Western Blot: CAD Antibody [NB100-61613] - Detection of Human and Mouse CAD on HeLa whole cell lysate using NB100-61613 . CAD was also immunoprecipitated by rabbit anti-CAD antibodies NB100-61612 and NB100-61614.](http://images.novusbio.com/fullsize/CAD-Antibody-Western-Blot-NB100-61613-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: CAD Antibody [NB100-61613] - Detection of Human and Mouse CAD on HeLa whole cell lysate using NB100-61613 . CAD was also immunoprecipitated by rabbit anti-CAD antibodies NB100-61612 and NB100-61614.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: CAD Antibody [NB100-61613] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CAD used at a dilution of 1:200 (1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit .](http://images.novusbio.com/fullsize/CAD-Antibody-Immunohistochemistry-NB100-61613-img0004.jpg)
![Western Blot: CAD Antibody [NBP2-58494] - Analysis using Anti-CAD antibody NBP2-58494 (A) shows similar pattern to independent antibody NBP2-56453 (B).](http://images.novusbio.com/fullsize/CAD-Antibody-Western-Blot-NBP2-58494-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: CAD Antibody [NBP2-58494] - Analysis using Anti-CAD antibody NBP2-58494 (A) shows similar pattern to independent antibody NBP2-56453 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: CAD Antibody [NBP2-58494] - Staining of human cell line U-251 MG shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/CAD-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58494-img0001.jpg)
![Western Blot: CAD Antibody [NBP2-56453] - Analysis using Anti-CAD antibody NBP2-56453 (A) shows similar pattern to independent antibody NBP2-58494 (B).](http://images.novusbio.com/fullsize/CAD-Antibody-Western-Blot-NBP2-56453-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: CAD Antibody [NBP2-56453] - Analysis using Anti-CAD antibody NBP2-56453 (A) shows similar pattern to independent antibody NBP2-58494 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: CAD Antibody [NBP2-56453] - Immunohistochemical staining of human tonsil shows cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/CAD-Antibody-Immunohistochemistry-NBP2-56453-img0003.jpg)
![Western Blot: CAD Antibody (SR1408) [NBP3-22169] - Western blot analysis using NBP3-22169 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22169_rabbit-cad-mab-sr1408-57202317215137.jpg "Western Blot: CAD Antibody (SR1408) [NBP3-22169] - Western blot analysis using NBP3-22169 in HeLa cell lysate.")