NL001) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane and cytoplasm. View our protocol for
Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">
NL001) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane and cytoplasm. View our protocol for
Fluorescent ICC Staining of Non-adherent Cells." alt="OX40 Ligand/TNFSF4 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse OX40 Ligand/TNFSF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1236) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog #
NL001) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane and cytoplasm. View our protocol for
Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />
MAB050) and sub-optimal amounts of Mouse CD3e Monoclonal Antibody (Catalog #
MAB484), Recombinant Mouse OX40 Ligand/TNFSF4 (Catalog #
1236-OX) stimulates IL-2 secretion in mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IL-2 Quantikine ELISA Kit (Catalog #
M2000). Under these conditions, IL-2 secretion elicited by Recombinant Mouse OX40 Ligand/TNFSF4 (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse OX40 Ligand/TNFSF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1236). The ND
50 is typically 0.2-0.6 µg/mL." class="big_lightbox">
MAB050) and sub-optimal amounts of Mouse CD3e Monoclonal Antibody (Catalog #
MAB484), Recombinant Mouse OX40 Ligand/TNFSF4 (Catalog #
1236-OX) stimulates IL-2 secretion in mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IL-2 Quantikine ELISA Kit (Catalog #
M2000). Under these conditions, IL-2 secretion elicited by Recombinant Mouse OX40 Ligand/TNFSF4 (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse OX40 Ligand/TNFSF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1236). The ND
50 is typically 0.2-0.6 µg/mL." class="big_thumb" />
NL001) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="OX40 Ligand/TNFSF4 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse OX40 Ligand/TNFSF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1236) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />
![ELISA: Human OX40 Ligand/TNFSF4 ELISA Kit (Colorimetric) [NBP2-62865] - Samples were spiked with high concentrations of Human OX40 Ligand/TNFSF4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-62865_human-ox40-ligand-tnfsf4-elisa-kit-colorimetric-132202416121373.jpg "ELISA: Human OX40 Ligand/TNFSF4 ELISA Kit (Colorimetric) [NBP2-62865] - Samples were spiked with high concentrations of Human OX40 Ligand/TNFSF4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human OX40 Ligand/TNFSF4 ELISA Kit (Colorimetric) [NBP2-62865] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-62865_human-ox40-ligand-tnfsf4-elisa-kit-colorimetric-43202495058.jpg)
![Western Blot: TNFSF4 Overexpression Lysate (Adult Normal) [NBL1-17169] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TNFSF4.](http://images.novusbio.com/fullsize/TNFSF4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17169-img0002.jpg "Western Blot: TNFSF4 Overexpression Lysate (Adult Normal) [NBL1-17169] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TNFSF4.")
![Flow Cytometry: OX40 Ligand/TNFSF4 Antibody (R4930 (Oxelumab)) - Chimeric [NBP2-52687] - Human peripheral blood leukocytes were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (isotype control, black line) or the rabbit IgG-chimeric version of R4930 (NBP2-52687, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/OX40-Ligand-TNFSF4-Antibody-R4930-Oxelumab-Chimeric-Flow-Cytometry-NBP2-52687-img0001.jpg "Flow Cytometry: OX40 Ligand/TNFSF4 Antibody (R4930 (Oxelumab)) - Chimeric [NBP2-52687] - Human peripheral blood leukocytes were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (isotype control, black line) or the rabbit IgG-chimeric version of R4930 (NBP2-52687, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
MAB050) and sub-optimal amounts of Mouse CD3e Monoclonal Antibody (Catalog # 
