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Products Related to Gene-ID: 7040

140 Results for "7040"
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BAF246

LAP (TGF-beta 1) Antibody [Biotin]

(17 Publications)
50 μg
BAF246
Disulfide-linked GARP/TGF-beta 1 complexes are released in the supernatant of T cells, but not 293 cells.A. Cells described in Figure 2 were lysed and immunoprecipitated (IP) with anti-GARP or anti-LAP antibodies. IP products were submitted to SDS-PAGE under non-reducing or reducing conditions, followed by WB with anti-LAP antibodies (top and middle panels), or anti-GARP antibodies (bottom panels). Pro-TGF-beta 1 and LAP homodimers in the top panels are not clearly resolved, but can be distinguished better with longer migrations or higher concentrations of polyacrylamide. The +/- 85-90 kDa bands that appear in the middle panel correspond to non-specific bands, or to incompletely reduced pro-TGF-beta 1. B. Cells (2x106/ml for murine and human T cells, 2.5x105/ml for transfected 293 cells) were incubated in serum free medium during 24 hours. Different cell concentrations were used to adjust for the different amounts of secreted TGF-beta 1 (see Figure 2). Human Th A2 and Jurkat cells were stimulated with anti-CD3/CD28 antibodies to increase secretion. Supernatants (0.5-10 µl) were analyzed by WB under non-reducing conditions with anti-GARP and anti-LAP antibodies. * Band that also appears when the secondary anti-IgG2b-HRP antibody is used alone (without anti-GARP antibody), due to cross reactivity against the anti-CD3/CD28 antibodies used for T cell stimulation. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0076186), licensed under a CC-BY license. Not internally tested by R&D Systems.
Hu
Species Glossary
Reactivity: Hu
WB
Applications: WB
Goat Polyclonal
Host: Goat Polyclonal
Biotin  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF-246-NA
Carrier Free AF-246-SP
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MAB7666

LAP (TGF-beta 1) Antibody (860206) [Unconjugated]

(12 Publications)
100 μg
MAB7666
Mouse splenocytes treated with 10 μg/mL Anti-CD3, 5 μg/mL Anti-CD28, 10 μg/mL Recombinant Human TGF‑ beta 1 (Catalog # 240-B), and 20 μg/mL Recombinant Mouse IL‑2 (Catalog # 402-IL) for 24 hours to induce T regulatory cells (iTregs) were stained with Rat Anti-Mouse CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rat Anti-Mouse LAP (TGF‑ beta 1) Monoclonal Antibody (Catalog # MAB7666) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
Mu
Species Glossary
Reactivity: Mu
Flow, CyTOF-ready
Applications: Flow, CyTOF-ready
Rat Monoclonal
Host: Rat Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB7666U-100UG
Alexa Fluor 405 FAB7666V-100UG
Alexa Fluor 488 FAB7666G-100UG
Alexa Fluor 532 FAB7666X
Alexa Fluor 594 FAB7666T-100UG
Alexa Fluor 647 FAB7666R-100UG
Alexa Fluor 700 FAB7666N-100UG
Alexa Fluor 750 FAB7666S-100UG
Allophycocyanin FAB7666A
Allophycocyanin/Cy7 FAB7666APCCY7
Biotin FAB7666B
CoraFluor 1 FAB7666CL1
DyLight 350 FAB7666D
DyLight 405 FAB7666E
DyLight 488 FAB7666K
DyLight 550 FAB7666L
DyLight 594 FAB7666M
DyLight 650 FAB7666W
DyLight 680 FAB7666Y
DyLight 755 FAB7666Z
FITC FAB7666F
HRP FAB7666H
Janelia Fluor 525 FAB7666JF525
Janelia Fluor 549 FAB7666I
Janelia Fluor 646 FAB7666J
Janelia Fluor 669 FAB7666JF669
PE FAB7666P
PE/Atto594 FAB7666PEATT594
PE/Cy5.5 FAB7666PECY55
PE/Cy7 FAB7666PECY7
PerCP FAB7666C
mFluor Violet 450 SE FAB7666MFV450
mFluor Violet 500 SE FAB7666MFV500
mFluor Violet 610 SE FAB7666MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB7666-SP
Carrier Free FAB7666V-100UG
Carrier Free FAB7666U-100UG
Carrier Free FAB7666T-100UG
Carrier Free FAB7666S-100UG
Carrier Free FAB7666R-100UG
Carrier Free FAB7666N-100UG
Carrier Free FAB7666G-100UG
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BAM2462

LAP (TGF-beta 1) Antibody (27240) [Biotin]

(1 Review)
(5 Publications)
50 μg
Hu
Species Glossary
Reactivity: Hu
ELISA(Det)
Applications: ELISA(Det)
Mouse Monoclonal
Host: Mouse Monoclonal
Biotin  
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MAB1835R

TGF beta 1/2/3 Antibody (1D11R) [Unconjugated]

(6 Publications)
100 μg
MAB1835R
Recombinant Human TGF-beta 1 (Catalog # 240-B) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Recombinant Human TGF-beta 1 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-TGF-beta 1, 2, 3 Monoclonal Antibody (Catalog # MAB1835R). The ND50 is typically0.25-1.25 µg/mL.Catalog # 240-B
All-Multi
Species Glossary
Reactivity: All-Multi
B/N
Applications: B/N
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB1835RU-100UG
Alexa Fluor 405 FAB1835RV-100UG
Alexa Fluor 488 FAB1835RG-100UG
Alexa Fluor 532 FAB1835RX-100UG
Alexa Fluor 594 FAB1835RT-100UG
Alexa Fluor 647 FAB1835RR-100UG
Alexa Fluor 700 FAB1835RN-100UG
Alexa Fluor 750 FAB1835RS-100UG
Allophycocyanin FAB1835RA
Biotin FAB1835RB
CoraFluor 1 FAB1835RCL1
DyLight 350 FAB1835RD
DyLight 405 FAB1835RE
DyLight 488 FAB1835RK
DyLight 550 FAB1835RL
DyLight 594 FAB1835RM
DyLight 650 FAB1835RW
DyLight 680 FAB1835RY
DyLight 755 FAB1835RZ
FITC FAB1835RF
HRP FAB1835RH
Janelia Fluor 525 FAB1835RJF525
Janelia Fluor 549 FAB1835RI
Janelia Fluor 646 FAB1835RJ
Janelia Fluor 669 FAB1835RJF669
PE FAB1835RP
PerCP FAB1835RC
mFluor Violet 450 SE FAB1835RMFV450
mFluor Violet 500 SE FAB1835RMFV500
mFluor Violet 610 SE FAB1835RMFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB1835R-SP
Carrier Free MAB1835R-500
Carrier Free MAB1835R-01M
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AF-246-NAN

LAP (TGF-beta 1) Antibody [Alexa Fluor® 700]

100 μg
Hu
Species Glossary
Reactivity: Hu
WB, B/N, ICC/IF
Applications: WB, B/N, ICC/IF
Alexa Fluor 700  
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AF-246-NAX

LAP (TGF-beta 1) Antibody [Alexa Fluor® 532]

100 μg
Hu
Species Glossary
Reactivity: Hu
WB, B/N, ICC/IF
Applications: WB, B/N, ICC/IF
Alexa Fluor 532  
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AF-246-NAS

LAP (TGF-beta 1) Antibody [Alexa Fluor® 750]

100 μg
Hu
Species Glossary
Reactivity: Hu
WB, B/N, ICC/IF
Applications: WB, B/N, ICC/IF
Alexa Fluor 750  
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AF-246-NAU

LAP (TGF-beta 1) Antibody [Alexa Fluor® 350]

100 μg
Hu
Species Glossary
Reactivity: Hu
WB, B/N, ICC/IF
Applications: WB, B/N, ICC/IF
Alexa Fluor 350  
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AF-246-NAV

LAP (TGF-beta 1) Antibody [Alexa Fluor® 405]

100 μg
Hu
Species Glossary
Reactivity: Hu
WB, B/N, ICC/IF
Applications: WB, B/N, ICC/IF
Alexa Fluor 405  
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AF-246-NAR

LAP (TGF-beta 1) Antibody [Alexa Fluor® 647]

100 μg
Hu
Species Glossary
Reactivity: Hu
WB, B/N, ICC/IF
Applications: WB, B/N, ICC/IF
Alexa Fluor 647  
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AF-246-NAT

LAP (TGF-beta 1) Antibody [Alexa Fluor® 594]

100 μg
Hu
Species Glossary
Reactivity: Hu
WB, B/N, ICC/IF
Applications: WB, B/N, ICC/IF
Alexa Fluor 594  
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AF-246-NAG

LAP (TGF-beta 1) Antibody [Alexa Fluor® 488]

100 μg
Hu
Species Glossary
Reactivity: Hu
WB, B/N, ICC/IF
Applications: WB, B/N, ICC/IF
Alexa Fluor 488  
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