| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74486 | |||
VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="TDO2 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human TDO2 Monoclonal Antibody (Catalog # MAB9768) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB9768-SP | |||
| Carrier Free | IC9768V-100UG | |||
| Carrier Free | IC9768U-100UG | |||
| Carrier Free | IC9768T-100UG | |||
| Carrier Free | IC9768S-100UG | |||
| Carrier Free | IC9768R-100UG | |||
| Carrier Free | IC9768N-100UG | |||
| Carrier Free | IC9768G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74485 | |||
![<b>Genetic Strategies Validation. </b>Western Blot: TDO2 Antibody [H00006999-B01P] - Analysis of TDO2 expression in transfected 293T cell line by TDO2 polyclonal antibody. Lane 1: TDO2 transfected lysate(44.66 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/TDO2-Antibody-Western-Blot-H00006999-B01P-img0005.jpg)
![Western Blot: TDO2 Antibody [H00006999-B01P] - Analysis of TDO2 expression in human liver.](http://images.novusbio.com/fullsize/TDO2-Antibody-Western-Blot-H00006999-B01P-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: TDO2 Antibody [H00006999-B01P] - Immunoreactivity of TDO in the SVZ, the olfactory bulb, & the cerebellum. A & B: TDO was not stained in the SVZ (A) & the olfactory bulb (OB) (B). The immunoreactivities for TDO in wild type & Tdo-/- mice were background level. C: In the cerebellum, the immunoreactivity for TDO was observed in granule cells & Purkinje cells of wild type mice, but these positive structures disappeared in Tdo-/- mice. The cytoarchitectural organization of Tdo-/- mice was the same as that of wild type mice. E, external plexiform layer; G, granule cell layer; Gm, glomerular layer; I, internal plexiform layer; LV, lateral ventricle; Mi, mitral cell layer; Mo, molecular layer; P, Purkinje cell layer; St, striatum; Su, subependymal zone. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20815922), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00006999-b01p_mouse-polyclonal-tdo2-antibody-310202415384127.jpg)
![Immunocytochemistry/ Immunofluorescence: TDO2 Antibody [H00006999-B01P] - Immunoreactivity of TDO in the SVZ, the olfactory bulb, & the cerebellum. A & B: TDO was not stained in the SVZ (A) & the olfactory bulb (OB) (B). The immunoreactivities for TDO in wild type & Tdo-/- mice were background level. C: In the cerebellum, the immunoreactivity for TDO was observed in granule cells & Purkinje cells of wild type mice, but these positive structures disappeared in Tdo-/- mice. The cytoarchitectural organization of Tdo-/- mice was the same as that of wild type mice. E, external plexiform layer; G, granule cell layer; Gm, glomerular layer; I, internal plexiform layer; LV, lateral ventricle; Mi, mitral cell layer; Mo, molecular layer; P, Purkinje cell layer; St, striatum; Su, subependymal zone. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20815922), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00006999-b01p_mouse-polyclonal-tdo2-antibody-310202415171317.jpg)
![Immunocytochemistry/ Immunofluorescence: TDO2 Antibody [H00006999-B01P] - Immunoreactivity of TDO in the SVZ, the olfactory bulb, & the cerebellum. A & B: TDO was not stained in the SVZ (A) & the olfactory bulb (OB) (B). The immunoreactivities for TDO in wild type & Tdo-/- mice were background level. C: In the cerebellum, the immunoreactivity for TDO was observed in granule cells & Purkinje cells of wild type mice, but these positive structures disappeared in Tdo-/- mice. The cytoarchitectural organization of Tdo-/- mice was the same as that of wild type mice. E, external plexiform layer; G, granule cell layer; Gm, glomerular layer; I, internal plexiform layer; LV, lateral ventricle; Mi, mitral cell layer; Mo, molecular layer; P, Purkinje cell layer; St, striatum; Su, subependymal zone. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20815922), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00006999-b01p_mouse-polyclonal-tdo2-antibody-310202415394527.jpg)
![Western Blot: TDO2 Antibody [H00006999-B01P] - Specificity of antibodies for tryptophan 2,3-dioxygenase (TDO) by Western blot (A) & immunofluorescent staining (B). A: The total protein (20 μg) of the dentate gyrus, extracted from the wild-type (lanes 1 & 3) & Tdo-/- mice (lane 2), was subjected to 4% to 12% gradient sodium dodecyl sulfate polyacrylamide gel electrophoresis, & transferred to polyvinylidene difluoride membranes. TDO was detected as described in the Methods section. Note that a 45-kDa band was detected with the anti-TDO antibody. The positions of molecular weight markers are shown on the left. Lane 3: negative control without the primary antibody. beta -Tubulin is a positive control for the Western blot. B: The hippocampal sections of wild-type & Tdo-/- mice were stained with anti-TDO antibody. In sections of Tdo-/- mice, immunofluorescence signals were not detected in granule cells, interneurons (arrowhead), CA1, or CA3 cells, suggesting that the secondary antibody used in this study bound specifically to the primary antibody (mouse IgG). g, granule cell layer; h, hilus. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20815922), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00006999-b01p_mouse-polyclonal-tdo2-antibody-310202415483615.jpg)
![Immunohistochemistry: TDO2 Antibody [H00006999-B01P] - Specificity of antibodies for tryptophan 2,3-dioxygenase (TDO) by Western blot (A) & immunofluorescent staining (B). A: The total protein (20 μg) of the dentate gyrus, extracted from the wild-type (lanes 1 & 3) & Tdo-/- mice (lane 2), was subjected to 4% to 12% gradient sodium dodecyl sulfate polyacrylamide gel electrophoresis, & transferred to polyvinylidene difluoride membranes. TDO was detected as described in the Methods section. Note that a 45-kDa band was detected with the anti-TDO antibody. The positions of molecular weight markers are shown on the left. Lane 3: negative control without the primary antibody. beta -Tubulin is a positive control for the Western blot. B: The hippocampal sections of wild-type & Tdo-/- mice were stained with anti-TDO antibody. In sections of Tdo-/- mice, immunofluorescence signals were not detected in granule cells, interneurons (arrowhead), CA1, or CA3 cells, suggesting that the secondary antibody used in this study bound specifically to the primary antibody (mouse IgG). g, granule cell layer; h, hilus. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20815922), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00006999-b01p_mouse-polyclonal-tdo2-antibody-310202415485113.jpg)
![Western Blot: TDO2 Antibody (OTI2A4) [NBP2-45995] -Analysis of overexpressedlysates(15ug per lane) from HEK293T cellstransfected with empty plasmid , lane1) , human TDO2 plasmid , lane 2),mouse TDO2 plasmid, lane 3) usinganti-TDO2 antibody (1:500). Figure B,Western blot analysis of the same samples asfigure A with anti-DDK antibody, 1:1000)](http://images.novusbio.com/fullsize/antibody/nbp2-45995_mouse-tdo2-mab-oti2a4-western-blot-342025113045..png "Western Blot: TDO2 Antibody (OTI2A4) [NBP2-45995] -Analysis of overexpressedlysates(15ug per lane) from HEK293T cellstransfected with empty plasmid , lane1) , human TDO2 plasmid , lane 2),mouse TDO2 plasmid, lane 3) usinganti-TDO2 antibody (1:500). Figure B,Western blot analysis of the same samples asfigure A with anti-DDK antibody, 1:1000)")
MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # 
![Immunohistochemistry-Paraffin: TDO2 Antibody (C2) [NBP3-41854] - DAB staining on IHC-P; Sample: Human Liver Tissue; Primary Ab: 10ug/ml NBP3-41854; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41854_mouse-tdo2-mab-c2-61220242126297.jpg "Immunohistochemistry-Paraffin: TDO2 Antibody (C2) [NBP3-41854] - DAB staining on IHC-P; Sample: Human Liver Tissue; Primary Ab: 10ug/ml NBP3-41854; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: TDO2 Antibody (C2) [NBP3-41854] - Sample: Lane1: Porcine Liver lysate; Lane2: Rat Liver lysate Primary Ab: 2ug/ml NBP3-41854; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41854_mouse-tdo2-mab-c2-612202421243225.jpg)
![Immunohistochemistry-Paraffin: TDO2 Antibody (C2) [NBP3-41854] - DAB staining on IHC-P; Sample: Human Skeletal muscle Tissue; Primary Ab: 10ug/ml NBP3-41854; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41854_mouse-tdo2-mab-c2-612202421121318.jpg)
![Western Blot: TDO2 Antibody (1D1) [NBP2-02002] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TDO2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TDO2.](http://images.novusbio.com/fullsize/TDO2-Antibody-1D1-Western-Blot-NBP2-02002-img0001.jpg "Western Blot: TDO2 Antibody (1D1) [NBP2-02002] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TDO2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TDO2.")
![Western Blot: TDO2 Antibody (C10) [NBP3-41855] - Sample: Lane1: Rat Liver lysate; Lane2: HepG2 cell lysate Primary Ab: 3ug/ml NBP3-41855; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41855_mouse-tdo2-mab-c10-612202421373010.jpg "Western Blot: TDO2 Antibody (C10) [NBP3-41855] - Sample: Lane1: Rat Liver lysate; Lane2: HepG2 cell lysate Primary Ab: 3ug/ml NBP3-41855; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Immunohistochemistry-Paraffin: TDO2 Antibody (C10) [NBP3-41855] - DAB staining on IHC-P; Sample: Human Kidney Tissue; Primary Ab: 20ug/ml NBP3-41855; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41855_mouse-tdo2-mab-c10-6122024215181.jpg)
![Western Blot: TDO2 Antibody [H00006999-D01P] - Analysis of TDO2 expression in Jurkat.](http://images.novusbio.com/fullsize/TDO2-Antibody-Western-Blot-H00006999-D01P-img0003.jpg "Western Blot: TDO2 Antibody [H00006999-D01P] - Analysis of TDO2 expression in Jurkat.")
![Western Blot: TDO2 Antibody [H00006999-D01P] - Analysis of TDO2 expression in transfected 293T cell line by TDO2 polyclonal antibody.Lane 1: TDO2 transfected lysate(47.90 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/TDO2-Antibody-Western-Blot-H00006999-D01P-img0004.jpg)
![Immunohistochemistry-Paraffin: TDO2 Antibody (C1) [NBP3-41732] - DAB staining on IHC-P; Sample: Human Liver Tissue; Primary Ab: 30ug/ml NBP3-41732; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41732_mouse-tdo2-mab-c1-61220242151876.jpg "Immunohistochemistry-Paraffin: TDO2 Antibody (C1) [NBP3-41732] - DAB staining on IHC-P; Sample: Human Liver Tissue; Primary Ab: 30ug/ml NBP3-41732; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: TDO2 Antibody (C1) [NBP3-41732] - Sample: Rat Liver lysate; Primary Ab: 3ug/ml NBP3-41732; Second Ab: 0.2ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41732_mouse-tdo2-mab-c1-612202420484722.jpg)
![Western Blot: TDO2 Antibody (C5) [NBP3-41944] - Sample: Rat Liver lysate Primary Ab: 2ug/ml NBP3-41944; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41944_mouse-tdo2-mab-c5-612202421351296.jpg "Western Blot: TDO2 Antibody (C5) [NBP3-41944] - Sample: Rat Liver lysate Primary Ab: 2ug/ml NBP3-41944; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Immunohistochemistry-Paraffin: TDO2 Antibody (C7) [NBP3-41946] - DAB staining on IHC-P; Sample: Human Kidney Tissue; Primary Ab: 20ug/ml NBP3-41946; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41946_mouse-tdo2-mab-c7-61220242195732.jpg "Immunohistochemistry-Paraffin: TDO2 Antibody (C7) [NBP3-41946] - DAB staining on IHC-P; Sample: Human Kidney Tissue; Primary Ab: 20ug/ml NBP3-41946; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
