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![Western Blot: Synuclein-alpha [p Ser129] Antibody [NB300-746] - Rat cortex lysate showing specific labeling of the ~15 k alpha synuclein protein phosphorylated at Ser129. Immunolabeling is blocked by the phosphopeptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).](http://images.novusbio.com/fullsize/Synuclein-alpha-[p-Ser129]-Antibody-Western-Blot-NB300-746-img0001.jpg "Western Blot: Synuclein-alpha [p Ser129] Antibody [NB300-746] - Rat cortex lysate showing specific labeling of the ~15 k alpha synuclein protein phosphorylated at Ser129. Immunolabeling is blocked by the phosphopeptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).")
![In vitro assay: Recombinant Human alpha-Synuclein Active, Monomer, (Type 1), A53T Mutant Protein [NBP3-14766] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show a limited increase in fluorescence (correlated to alpha synuclein aggregation) over time in A53T alpha synuclein monomers (NBP3-14766). A much greater increase in fluorescence is seen when 100 uM monomer (NBP3-14766) is combined with 10 nM of fibrils as the fibrils seed the formation of new fibrils from the pool of active monomers. Thioflavin T ex = 450 nm, em = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Monomer-Type-1-A53T-Mutant-Protein-In-vitro-assay-NBP3-14766-img0001.jpg "In vitro assay: Recombinant Human alpha-Synuclein Active, Monomer, (Type 1), A53T Mutant Protein [NBP3-14766] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show a limited increase in fluorescence (correlated to alpha synuclein aggregation) over time in A53T alpha synuclein monomers (NBP3-14766). A much greater increase in fluorescence is seen when 100 uM monomer (NBP3-14766) is combined with 10 nM of fibrils as the fibrils seed the formation of new fibrils from the pool of active monomers. Thioflavin T ex = 450 nm, em = 485 nm.")
![SDS-Page: Recombinant Human alpha-Synuclein Active, Monomer, (Type 1), A53T Mutant Protein [NBP3-14766] - SDS-PAGE of ~14 kDa A53T alpha-Synuclein Monomer (NBP3-14766)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Monomer-Type-1-A53T-Mutant-Protein-SDS-Page-NBP3-14766-img0002.jpg)
![SDS-Page: Recombinant Human alpha-Synuclein Oligomers, (Dopamine HCL Stabilized) Protein [NBP3-14772] - SDS-PAGE of EGCG-stabilized alpha synuclein oligomers (NBP3-14772)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Oligomers-Dopamine-HCL-Stabilized-Protein-SDS-Page-NBP3-14772-img0001.jpg "SDS-Page: Recombinant Human alpha-Synuclein Oligomers, (Dopamine HCL Stabilized) Protein [NBP3-14772] - SDS-PAGE of EGCG-stabilized alpha synuclein oligomers (NBP3-14772)")
![SDS-Page: Recombinant Human alpha-Synuclein Oligomers, (Dopamine HCL Stabilized) Protein [NBP3-14772] - Native SDS-PAGE of EGCG-stabilized alpha synuclein oligomers (NBP3-14772)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Oligomers-Dopamine-HCL-Stabilized-Protein-SDS-Page-NBP3-14772-img0002.jpg)
![In vitro assay: Recombinant Rat alpha-Synuclein Active, Monomer Protein [NBP3-14773] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha synuclein monomers and fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. The Rat alpha-Synuclein monomer (NBP3-14773) is very active and was able to form more beta-sheet structure alone than with the combination of monomer and fibril. In combination, the fibril is the majority of the seed.](http://images.novusbio.com/fullsize/Recombinant-Rat-alpha-Synuclein-Active--Monomer-Protein-In-vitro-assay-NBP3-14773-img0001.jpg "In vitro assay: Recombinant Rat alpha-Synuclein Active, Monomer Protein [NBP3-14773] - Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha synuclein monomers and fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. The Rat alpha-Synuclein monomer (NBP3-14773) is very active and was able to form more beta-sheet structure alone than with the combination of monomer and fibril. In combination, the fibril is the majority of the seed.")
![SDS-Page: Recombinant Rat alpha-Synuclein Active, Monomer Protein [NBP3-14773] - SDS-PAGE of Rat alpha-Synuclein Protein Monomer (NBP3-14773)](http://images.novusbio.com/fullsize/Recombinant Rat alpha-Synuclein Active- Monomer Protein-SDS-Page-NBP3-14773-img0002.jpg)
![ELISA: Mouse alpha-Synuclein ELISA Kit (Colorimetric) [NBP3-08174] - Image from verified customer review.](http://images.novusbio.com/fullsize/nbp3-08174_mouse-alpha-synuclein-elisa-kit-colorimetric-elisa-1962023111722..jpg "ELISA: Mouse alpha-Synuclein ELISA Kit (Colorimetric) [NBP3-08174] - Image from verified customer review.")
![ELISA: Mouse alpha-Synuclein ELISA Kit (Colorimetric) [NBP3-08174] - DRC. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp3-08174_mouse-alpha-synuclein-elisa-kit-colorimetric-258202314226.png)
![ELISA: Mouse alpha-Synuclein ELISA Kit (Colorimetric) [NBP3-08174] - Samples were spiked with high concentrations of Mouse alpha-Synuclein and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-08174_mouse-alpha-synuclein-elisa-kit-colorimetric-132202416225238.jpg)
![ELISA: Mouse alpha-Synuclein ELISA Kit (Colorimetric) [NBP3-08174] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-08174_mouse-alpha-synuclein-elisa-kit-colorimetric-4320249141641.jpg)
![Western Blot: alpha-Synuclein [p Tyr136] Antibody [NBP3-05404] - Western blot analysis of extracts of Mouse brain, using alpha-Synuclein antibody (NBP3-05404) at 1:1000 dilution.Mouse brain were treated by lambda-PP mixed solution (1ul) at 30C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/alpha-Synuclein [p Tyr136] Antibody-Western Blot-NBP3-05404-img0002.jpg "Western Blot: alpha-Synuclein [p Tyr136] Antibody [NBP3-05404] - Western blot analysis of extracts of Mouse brain, using alpha-Synuclein antibody (NBP3-05404) at 1:1000 dilution.Mouse brain were treated by lambda-PP mixed solution (1ul) at 30C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein [p Tyr136] Antibody [NBP3-05404] - Immunofluorescence analysis of methanol-fixed HeLa cells using alpha-Synuclein antibody (NBP3-05404).](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Tyr136]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05404-img0001.jpg)
![Western Blot: Synuclein alpha/beta Antibody (SR1281) [NBP3-21662] - Western blot analysis using NBP3-21662 in Mouse brain tissue lysate.](http://images.novusbio.com/fullsize/nbp3-21662_rabbit-synuclein-alpha-beta-mab-sr1281-5720231595518.jpg "Western Blot: Synuclein alpha/beta Antibody (SR1281) [NBP3-21662] - Western blot analysis using NBP3-21662 in Mouse brain tissue lysate.")
![In vitro assay: Recombinant Human alpha-Synuclein Active, Monomer, (Type 1) Acetyl (N-Term) Protein [NBP3-14768] - Thioflavin T assay for N-terminal acetylated alpha synuclein monomers (NBP3-14768) and pre-formed fibrils (PFFs) . Increased fluorescence, indicative of beta-sheet formation, was seen when 50 ug N-acetylated monomer and 5 ug N-acetylated fibril were combined, compared to monomer or fibril alone.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Monomer-Type-1-Acetyl-N-Term-Protein-In-vitro-assay-NBP3-14768-img0001.jpg "In vitro assay: Recombinant Human alpha-Synuclein Active, Monomer, (Type 1) Acetyl (N-Term) Protein [NBP3-14768] - Thioflavin T assay for N-terminal acetylated alpha synuclein monomers (NBP3-14768) and pre-formed fibrils (PFFs) . Increased fluorescence, indicative of beta-sheet formation, was seen when 50 ug N-acetylated monomer and 5 ug N-acetylated fibril were combined, compared to monomer or fibril alone.")
![Western Blot: alpha-Synuclein Antibody (017) [NBP2-90051] - Lane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:16 kDaObserved band size:18 kDa](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-017-Western-Blot-NBP2-90051-img0001.jpg "Western Blot: alpha-Synuclein Antibody (017) [NBP2-90051] - Lane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:16 kDaObserved band size:18 kDa")
![<b>Genetic Strategies Validation. </b>Western Blot: alpha-Synuclein Antibody (017) [NBP2-90051] - NBP2-90051 at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: alpha-Synuclein knockout Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size: 16 kDa](http://images.novusbio.com/fullsize/nbp2-90051_rabbit-monoclonal-alpha-synuclein-antibody-017-136202422345017.png)
![Western Blot: alpha-Synuclein [p Ser129] Antibody [NBP3-05429] - Western blot analysis of extracts from mouse brain tissue, using alpha-Synuclein antibody (NBP3-05429). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Western-Blot-NBP3-05429-img0001.jpg "Western Blot: alpha-Synuclein [p Ser129] Antibody [NBP3-05429] - Western blot analysis of extracts from mouse brain tissue, using alpha-Synuclein antibody (NBP3-05429). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
![ELISA: Human alpha-Synuclein ELISA Kit (Chemiluminescence) [NBP2-62771] - Samples were spiked with high concentrations of Human alpha-Synuclein and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-62771_human-alpha-synuclein-elisa-kit-chemiluminescence-13220241613097.jpg "ELISA: Human alpha-Synuclein ELISA Kit (Chemiluminescence) [NBP2-62771] - Samples were spiked with high concentrations of Human alpha-Synuclein and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human alpha-Synuclein ELISA Kit (Chemiluminescence) [NBP2-62771] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-62771_human-alpha-synuclein-elisa-kit-chemiluminescence-4320249192863.jpg)
![ELISA: Human alpha-Synuclein ELISA Kit (Colorimetric) [NBP2-62748] - Samples were spiked with high concentrations of Human alpha-Synuclein and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-62748_human-alpha-synuclein-elisa-kit-colorimetric-1322024162043.jpg "ELISA: Human alpha-Synuclein ELISA Kit (Colorimetric) [NBP2-62748] - Samples were spiked with high concentrations of Human alpha-Synuclein and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human alpha-Synuclein ELISA Kit (Colorimetric) [NBP2-62748] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-62748_human-alpha-synuclein-elisa-kit-colorimetric-432024941523.jpg)