| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB13382-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB13383-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-47338-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41288-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1338-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB11376-SP | |||
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody (970106) [MAB13382] - Analysis using immersion fixed SK-Mel-28 human cytoplasm using SNCA antibody.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-970106-Immunocytochemistry-Immunofluorescence-MAB13382-img0001.jpg "Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody (970106) [MAB13382] - Analysis using immersion fixed SK-Mel-28 human cytoplasm using SNCA antibody.")
![Western Blot: pan-Synuclein Antibody [NBP2-47338] - Analysis in human cell lines SK-MEL-30 and MCF-7 using anti-SNCA antibody. Corresponding SNCA RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.](http://images.novusbio.com/fullsize/pan-Synuclein-Antibody-Western-Blot-NBP2-47338-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: pan-Synuclein Antibody [NBP2-47338] - Analysis in human cell lines SK-MEL-30 and MCF-7 using anti-SNCA antibody. Corresponding SNCA RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: pan-Synuclein Antibody [NBP2-47338] - Staining in human cerebral cortex and liver tissues using anti-SNCA antibody. Corresponding SNCA RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/pan-Synuclein-Antibody-Immunohistochemistry-Paraffin-NBP2-47338-img0007.jpg)
![Western Blot: pan-Synuclein Antibody [NBP2-47338] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/pan-Synuclein-Antibody-Western-Blot-NBP2-47338-img0002.jpg)
![Immunohistochemistry: pan-Synuclein Antibody [NBP2-47338] - Staining of human cerebral cortex shows strongly immunoreactivity neuropil and weak cytoplasmic positivity in neuronal cells, glial cells.](http://images.novusbio.com/fullsize/pan-Synuclein-Antibody-Immunohistochemistry-NBP2-47338-img0001.jpg)
![Immunohistochemistry-Paraffin: pan-Synuclein Antibody [NBP2-47338] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/pan-Synuclein-Antibody-Immunohistochemistry-Paraffin-NBP2-47338-img0005.jpg)
![Immunohistochemistry-Paraffin: pan-Synuclein Antibody [NBP2-47338] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/pan-Synuclein-Antibody-Immunohistochemistry-Paraffin-NBP2-47338-img0006.jpg)
![Western Blot: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Western Blot analysis of Human Alpha Synuclein showing detection of alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). Lane 1: MW ladder. Lane 2: 0.5 ug human alpha synuclein monomer (SPR-321). Lane 3: 2 ug human alpha synuclein monomer (SPR-321). Lane 4: 0.5 ug human alpha synuclein PFFs (SPR-322). Lane 5: 2 ug human alpha synuclein PFFs (SPR-322). Lane 6: 15 ug human Parkinson's Disease brain lysate.. Block: 5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. NBP3-18266 does not detect unphosphorylated alpha synuclein.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Western-Blot-NBP3-18266-img0004.jpg "Western Blot: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Western Blot analysis of Human Alpha Synuclein showing detection of alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). Lane 1: MW ladder. Lane 2: 0.5 ug human alpha synuclein monomer (SPR-321). Lane 3: 2 ug human alpha synuclein monomer (SPR-321). Lane 4: 0.5 ug human alpha synuclein PFFs (SPR-322). Lane 5: 2 ug human alpha synuclein PFFs (SPR-322). Lane 6: 15 ug human Parkinson's Disease brain lysate.. Block: 5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. NBP3-18266 does not detect unphosphorylated alpha synuclein.")
![Immunohistochemistry: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - C57/BL6 mice were injected with sonicated recombinant mouse alpha synuclein monomers or fibrils at 8 weeks of age. Mice were unilaterally injected in the dorsal striatum (bregma AP + 0.2 mm, L +/1 2.0 mm, V - 3.0 mm) and sacrificed 30 days post-injection. (A) 1.25 uL mouse alpha synuclein monomers. (B) 2.5 uL mouse alpha synuclein monomers. (C) 2.5 ug alpha synuclein PFFs. (C) 5 ug alpha synuclein PFFs Inset: PBS (negative control). Primary antibody: Anti-alpha-Synuclein (NBP3-18266) at 1:10 000. Secondary antibody: anti-rabbit HRP. Mice injected with PFF displayed alpha synuclein staining in the striatum and cortex and contralateral to the injection site.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Immunohistochemistry-NBP3-18266-img0002.jpg)
![Western Blot: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Western Blot analysis of Mouse Brain showing detection of alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). Lane 1: MW ladder. Lane 2: Mouse brain. Load: 15 ug. Block: 5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Multiple bands due to phosphorylation.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Western-Blot-NBP3-18266-img0005.jpg)
![Immunohistochemistry: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Immunohistochemistry analysis using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). C57/BL6 mice were injected with 5 ug sonicated mouse recombinant alpha synuclein PFFs at 8 weeks of age. Mice were unilaterally injected in the dorsal striatum (bregma AP + 0.2 mm, L +/1 2.0 mm, V - 3.0 mm) and sacrificed 30 days post-injection. (A) contralateral cortex. (B) ipsilateral cortex. (C) contralateral striatum. (D) ipsilateral striatum. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:10000. Secondary Antibody: anti-rabbit HRP. Courtesy of: Porsolt.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Immunohistochemistry-NBP3-18266-img0001.jpg)
![Dot Blot: alpha-Synuclein [p Ser 129] Antibody (J18) [NBP3-18266] - Dot Blot analysis using Rabbit Anti-alpha-Synuclein Monoclonal Antibody, Clone J18 (NBP3-18266). Tissue: alpha synuclein peptide. Primary Antibody: Rabbit Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18266) at 1:500 for 2 hours at RT with shaking . Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking . Phospho peptide sequence: AYEMP-pS-EEGYQ. Non-phospho peptide sequence: AYEMPSEEGYQ. This sequence is the same for human, mouse, and rat.](http://images.novusbio.com/fullsize/alpha-Synuclein-p-Ser-129-Antibody-J18-Dot-Blot-NBP3-18266-img0003.jpg)
![Western Blot: alpha-Synuclein Antibody (3C11) [NBP3-18258] - Western Blot analysis of Human Brain showing detection of 14 kDa alpha-Synuclein protein using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 3C11 (NBP3-18258). Lane 1: Molecular Weight Ladder (MW). Lane 2: Parkinson brain cell lystate. Lane 3: Human brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18258) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): 100 kDa (oligomer).](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3C11-Western-Blot-NBP3-18258-img0003.jpg "Western Blot: alpha-Synuclein Antibody (3C11) [NBP3-18258] - Western Blot analysis of Human Brain showing detection of 14 kDa alpha-Synuclein protein using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 3C11 (NBP3-18258). Lane 1: Molecular Weight Ladder (MW). Lane 2: Parkinson brain cell lystate. Lane 3: Human brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18258) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): 100 kDa (oligomer).")
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody (3C11) [NBP3-18258] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 3C11 (NBP3-18258). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18258) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: weak; Nucleus: Med. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) alpha-Synuclein Antibody. (D) Composite.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3C11-Immunocytochemistry-Immunofluorescence-NBP3-18258-img0001.jpg)
![Immunohistochemistry: alpha-Synuclein Antibody (3C11) [NBP3-18258] - Immunohistochemistry analysis using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 3C11 (NBP3-18258). Tissue: cerebellum. Species: Rat. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18258) at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) alpha-Synuclein Antibody (D) Composite.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3C11-Immunohistochemistry-NBP3-18258-img0002.jpg)
![Western Blot: alpha-Synuclein Antibody (3C11) [NBP3-18258] - Western Blot analysis of Mouse, Rat Brain showing detection of 14 kDa alpha-Synuclein protein using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 3C11 (NBP3-18258). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain cell lysate. Lane 3: Rat brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18258) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): ~30 kDa (dimer).](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3C11-Western-Blot-NBP3-18258-img0004.jpg)
![Western Blot: alpha-Synuclein Antibody [NBP2-41288] - Analysis of 1 ng of recombinant human SNCA with SNCA antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Western-Blot-NBP2-41288-img0004.jpg "Western Blot: alpha-Synuclein Antibody [NBP2-41288] - Analysis of 1 ng of recombinant human SNCA with SNCA antibody at 1 ug/ml.")
![Western Blot: alpha-Synuclein Antibody [NBP2-41288] - Western blot analysis of SNCA in mouse cerebellum tissue lysate with SNCA antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Western-Blot-NBP2-41288-img0001.jpg)
![Immunohistochemistry: alpha-Synuclein Antibody - BSA Free [NBP2-41288] - Immunohistochemistry of alpha-Synuclein in rat brain tissue with alpha-Synuclein antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41288_rabbit-polyclonal-alpha-synuclein-antibody-2711202419241375.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha-Synuclein Antibody - BSA Free [NBP2-41288] - Immunofluorescence of alpha-Synuclein in rat brain tissue with alpha-Synuclein antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41288_rabbit-polyclonal-alpha-synuclein-antibody-27112024201327.jpg)
![N/A alpha-Synuclein [Biotin]](http://images.novusbio.com/fullsize2/DATA_alphaSynuclein_DY133805_ELISA_2354.jpg "N/A alpha-Synuclein [Biotin]")
![Immunocytochemistry/Immunofluorescence: Synuclein alpha/beta Antibody (3B6) [NBP1-22957] - Analysis of LNCaP cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti human Synuclein alpha/beta Antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3B6-Immunocytochemistry-Immunofluorescence-NBP1-22957-img0003.jpg "Immunocytochemistry/Immunofluorescence: Synuclein alpha/beta Antibody (3B6) [NBP1-22957] - Analysis of LNCaP cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti human Synuclein alpha/beta Antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).")
![Western Blot: Synuclein alpha/beta Antibody (3B6) [NBP1-22957] - The recombinant human synuclein family (alpha-,beta- and gamma-) (each 20ng), Mouse brain and Rat brain (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human alpha,beta-synuclein (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3B6-Western-Blot-NBP1-22957-img0002.jpg)
![Flow Cytometry: Synuclein alpha/beta Antibody (3B6) [NBP1-22957] - Analysis of alpha, beta-Synuclein in C6 cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3B6-Flow-Cytometry-NBP1-22957-img0006.jpg)
![Flow Cytometry: Synuclein alpha/beta Antibody (3B6) [NBP1-22957] - Analysis of LNCaP cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3B6-Flow-Cytometry-NBP1-22957-img0004.jpg)
![Flow Cytometry: Synuclein alpha/beta Antibody (3B6) [NBP1-22957] - Analysis of alpha, beta-Synuclein in U87MG cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3B6-Flow-Cytometry-NBP1-22957-img0005.jpg)
![Electron Microscopy: Recombinant Human alpha-Synuclein Oligomers, (Dopamine HCL Stabilized) Protein [NBP3-14771] - TEM of Human Recombinant alpha-Synuclein Oligomers (Dopamine HCL Stabilized) (NBP3-14771)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Oligomers-Dopamine-HCL-Stabilized-Protein-Electron-Microscopy-NBP3-14771-img0001.jpg "Electron Microscopy: Recombinant Human alpha-Synuclein Oligomers, (Dopamine HCL Stabilized) Protein [NBP3-14771] - TEM of Human Recombinant alpha-Synuclein Oligomers (Dopamine HCL Stabilized) (NBP3-14771)")
![Electron Microscopy: Recombinant Human alpha-Synuclein Oligomers, (Dopamine HCL Stabilized) Protein [NBP3-14771] - TEM of Human Recombinant alpha-Synuclein Oligomers (Dopamine HCL Stabilized) (NBP3-14771)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Oligomers-Dopamine-HCL-Stabilized-Protein-Electron-Microscopy-NBP3-14771-img0002.jpg)
![SDS-Page: Recombinant Human alpha-Synuclein Oligomers, (Dopamine HCL Stabilized) Protein [NBP3-14771] - Coomassie gel stain of human dopamine-stabilized alpha synuclein oligomers (NBP3-14771). Load: 2 ug.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Oligomers-Dopamine-HCL-Stabilized-Protein-SDS-Page-NBP3-14771-img0004.jpg)
![Product Image: Recombinant Human alpha-Synuclein Oligomers, (Dopamine HCL Stabilized) Protein [NBP3-14771] - HPLC of alpha-Synuclein Protein Oligomers (NBP3-14771) ran down a size exclusion column. Wavelength: 280 nm.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Oligomers-Dopamine-HCL-Stabilized-Protein-Product-Image-NBP3-14771-img0003.jpg)
![Western Blot: alpha-Synuclein Antibody (4F1) [NBP3-18259] - Western Blot analysis of Human, Mouse, Rat Brain showing detection of 14 kDa alpha-Synuclein protein using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 4F1 (NBP3-18259). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain cell lysate. Lane 3: Rat brain cell lysate. Lane 4: Human brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18259) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): ~40 kDa (trimer).](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-4F1-Western-Blot-NBP3-18259-img0002.jpg "Western Blot: alpha-Synuclein Antibody (4F1) [NBP3-18259] - Western Blot analysis of Human, Mouse, Rat Brain showing detection of 14 kDa alpha-Synuclein protein using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 4F1 (NBP3-18259). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain cell lysate. Lane 3: Rat brain cell lysate. Lane 4: Human brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18259) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): ~40 kDa (trimer).")
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody (4F1) [NBP3-18259] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 4F1 (NBP3-18259). Tissue: Primary hippocampal neurons treated with active alpha-Synuclein Protein Aggregate (SPR-322) at 4 ug/ml to induce fibrils. Species: Rat. Fixation: 4% paraformaldehyde. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18259) at 1:200 for 24 hours at 4C. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:700 for 1 hour at RT. Counterstain: Guinea Pig Anti-NeuN (red) neuronal marker (Donkey Anti-Guinea Pig Alexa Fluor 647 1:700); DAPI (blue) nuclear stain at 1:6000, 1:3000 for 60 min at RT, 5 min at RT. Magnification: 20X. (A) DAPI (blue) nuclear stain. (B) NeuN neuronal marker (red). (C) alpha-Synuclein Antibody. (D) Composite.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-4F1-Immunocytochemistry-Immunofluorescence-NBP3-18259-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody (4F1) [NBP3-18259] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 4F1 (NBP3-18259). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18259) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: medium; Nucleus: strong. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) alpha-Synuclein Antibody. (D) Composite.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-4F1-Immunocytochemistry-Immunofluorescence-NBP3-18259-img0001.jpg)