DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to cell membrane of microglia. Protocol available in COMET™ Panel Builder." alt="P2Y12/P2RY12 was detected in immersion fixed paraffin-embedded sections of human Cortex using Rabbit Anti-human P2Y12/P2RY12, Polyclonal Antibody (Catalog # NBP2-33870) at 1:100 at 37°Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH).Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to cell membrane of microglia. Protocol available in COMET™ Panel Builder." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33870-25ul | |||
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP2-33870] - Staining in human cerebral cortex and liver tissues . Corresponding P2RY12 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NBP2-33870-img0014.jpg)
![Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP2-33870] - Staining of human cerebral cortex shows strong cytoplasmic positivity in microglia.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NBP2-33870-img0012.jpg)
![Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP2-33870] - Staining of human liver shows no positivity as expected.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NBP2-33870-img0013.jpg)
![Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP2-33870] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NBP2-33870-img0016.jpg)
![Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP2-33870] - Staining of human cerebellum shows strong cytoplasmic positivity in microglia.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NBP2-33870-img0017.jpg)
![Immunohistochemistry: P2Y12/P2RY12 Antibody [NBP2-33870] - Images demonstrating colocalization of CD105/MAB1097 with microglial markers (a) panels A, B Low magnification images of high-plaque (HP) case (panel A) & AD case (panel B) showing staining with CD105/MAB1097 (purple arrows) & IBA-1 (brown arrows) in MTG sections. Both images at same magnification: scale bars represent 100 μm. (b) (panel A) CD105 microglia (purple arrows) with ramified morphology stained with antibody to P2RY12 (brown arrow) in low-plaque (LP) case. (panel B) CD105 microglia (purple arrows) & HLA-DR (brown arrow) in AD case. (panel C) CD105 microglia (purple arrow) & CD45 (brown arrow) in AD case. All sections were from MTG. Images at same magnification: scale bars represent 50 μm. (c) (panel A–E). Patterns of staining of CD105 (purple) & IBA-1 (brown) in MTG sections of cases with progressively increasing pathology from LP to AD. (panel F). CD105-positive microglia in hippocampus (HPC) of AD case. Images at same magnification: scale bars represent 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31340569), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-33870_rabbit-p2y12-p2ry12-pab-310202416114763.jpg)
