Products Related to Gene-ID: 6469

HAF005). A specific band was detected for Sonic Hedgehog/Shh at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF005). A specific band was detected for Sonic Hedgehog/Shh at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human brain tissue and mouse brain tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse Sonic Hedgehog/Shh N-Terminus Monoclonal Antibody (Catalog # MAB464) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Sonic Hedgehog/Shh at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

BT-SHH-AFL) grades of Recombinant Mouse Sonic Hedgehog/Shh as measured in the alkaline phosphatase production using C3H10T1/2 mouse embryonic fibroblast cells (orange and green, respectively)." class="big_lightbox"> BT-SHH-AFL) grades of Recombinant Mouse Sonic Hedgehog/Shh as measured in the alkaline phosphatase production using C3H10T1/2 mouse embryonic fibroblast cells (orange and green, respectively)." class="big_thumb" />

5748), anti-TH (Catalog # AF7566), and anti-TUJ1 (Catalog # MAB1195), followed by secondary antibody staining with anti-mouse IgG Alexa Fluor 488 conjugated antibody and anti-sheep IgG NorthernLights NL557-Conjugated Antibody (Catalog # NL010)." class="big_lightbox"> 5748), anti-TH (Catalog # AF7566), and anti-TUJ1 (Catalog # MAB1195), followed by secondary antibody staining with anti-mouse IgG Alexa Fluor 488 conjugated antibody and anti-sheep IgG NorthernLights NL557-Conjugated Antibody (Catalog # NL010)." class="big_thumb" />

BT-SHH-GMP), Animal-Free (Catalog # BT-SHH-AFL) and RUO grades of Recombinant Mouse Sonic Hedgehog/Shh as measured in the alkaline phosphatase production using C3H10T1/2 mouse embryonic fibroblast cells (orange, green, red, respectively)." class="big_lightbox"> BT-SHH-GMP), Animal-Free (Catalog # BT-SHH-AFL) and RUO grades of Recombinant Mouse Sonic Hedgehog/Shh as measured in the alkaline phosphatase production using C3H10T1/2 mouse embryonic fibroblast cells (orange, green, red, respectively)." alt="Equivalent bioactivity of GMP (BT-SHH-GMP), Animal-Free (Catalog # BT-SHH-AFL) and RUO grades of Recombinant Mouse Sonic Hedgehog/Shh as measured in the alkaline phosphatase production using C3H10T1/2 mouse embryonic fibroblast cells (orange, green, red, respectively)." class="big_thumb" />

MAB10939) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Sonic Hedgehog/Shh Monoclonal Antibody (Catalog # MAB109391) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> MAB10939) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Sonic Hedgehog/Shh Monoclonal Antibody (Catalog # MAB109391) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human Sonic Hedgehog/Shh protein was serially diluted 2-fold and captured by Mouse Anti-Human Sonic Hedgehog/Shh Monoclonal Antibody (Catalog # MAB10939) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Sonic Hedgehog/Shh Monoclonal Antibody (Catalog # MAB109391) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

DY990). Mouse Anti-Human Sonic Hedgehog/Shh Monoclonal Antibody (Catalog # MAB109391) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> DY990). Mouse Anti-Human Sonic Hedgehog/Shh Monoclonal Antibody (Catalog # MAB109391) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human Sonic Hedgehog/Shh protein was serially diluted 2-fold and captured by Mouse Anti-Human Sonic Hedgehog/Shh Monoclonal Antibody (Catalog # MAB10939) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Sonic Hedgehog/Shh Monoclonal Antibody (Catalog # MAB109391) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />