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![Western Blot: Ribosomal protein L26 Antibody [NB100-2130] - Detection of Human and Mouse RPL26 on HeLa whole cell lysate using NB100-2130. RPL26 was also immunoprecipitated by rabbit anti-RPL26 antibody NB100-2131.](http://images.novusbio.com/fullsize/Ribosomal-protein-L26-Antibody-Western-Blot-NB100-2130-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Ribosomal protein L26 Antibody [NB100-2130] - Detection of Human and Mouse RPL26 on HeLa whole cell lysate using NB100-2130. RPL26 was also immunoprecipitated by rabbit anti-RPL26 antibody NB100-2131.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Ribosomal protein L26 Antibody [NB100-2130] - Demonstration of the binding of RPL26, RPL4, RPL8, & RPS9 to rs6032664. (A) Allele-imbalanced binding of RPL26, RPL4, RPL8 & RPS9 to the T (risk allele) & A (non-risk allele) of rs6032664 with the statistical analysis based on the relative density. PARP-1 was used as internal loading control & RSRC2 was used as a positive control. (B) Luciferase reporter assays with the risk allele T of rs6032664 demonstrate that RPL26, RPL4, RPL8, & RPS9 are the transcriptional regulators. si: siRNA. (C). ChIP assay showing endogenous binding of RPL26 to rs6032664. (mean ± SD, n = 3 biological replicates, Student t-test with 2 tails). sh: shRNA. NS: not significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33371207), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2130_rabbit-polyclonal-ribosomal-protein-l26-antibody-31020241539746.jpg)
![Western Blot: Ribosomal protein L26 Antibody [NB100-2130] - Regulation of disease-associated risk genes STAT4, TRAF1, ICAM1, & CD86 by RPL26 via disease-associated TRN linked by CD40-induced NF-kappa B signal pathway. (A) Western blots showing inactivation of NF-kappa B p65 by dephosphorylation upon CD40L activation in the RPL26 siRNA knockdown FLS. Statistical analysis based on the relative density is shown. (B) real time PCR showing downregulation of four disease-associated risk genes STAT4, TRAF1, ICAM1, & CD86 as the direct targets of NF-kappa B p65, (C) real time PCR showing downregulation of IL6 & IL1 beta as the direct targets of NF-kappa B p65. si: siRNA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33371207), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2130_rabbit-polyclonal-ribosomal-protein-l26-antibody-310202415384147.jpg)
![Western Blot: Ribosomal protein L26 Antibody [NB100-2130] - Demonstration of RPL26, RPL4, RPL8, & RPS9, as CD40 transcription regulators in human B cells. (A) Knockdown of RPL26, RPL4, RPL8, & RPS9, in human BL2 cells using shRNA as shown by real time PCR. (B) Western blots showing downregulation of RPL26, RPL4, RPL8, & RPS9 results in decreased expression of CD40, but not CCR6 in BL2 cells. Whole cell protein was used for Western blots. (C) Statistical analysis of Western blots in (B) based on relative density. NS: not significant. (D) Down-regulation of CD40 expression on mRNA levels in the knockdown cells, suggesting a transcriptional regulation of CD40 expression. alpha -Tubulin was used for loading control. CCR6 was used as a negative control for translational regulation. (E) FACS analysis of CD40 surface expression in the RPL26, RPL4, RPL8, & RPS9 shRNA knockdown BL2 cells. Scrambled: scrambled shRNA control. sh: shRNA. **: p value < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33371207), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2130_rabbit-polyclonal-ribosomal-protein-l26-antibody-310202415525288.jpg)
![Western Blot: Ribosomal protein L26 Antibody [NBP1-80812] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/RPL26L1-Antibody-Western-Blot-NBP1-80812-img0006.jpg "Western Blot: Ribosomal protein L26 Antibody [NBP1-80812] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp")
![Immunocytochemistry/Immunofluorescence: Ribosomal protein L26 Antibody [NBP1-80812] - Staining of human cell line A-431 shows localization to nucleoli, cytosol & endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RPL26L1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80812-img0005.jpg)
![Immunohistochemistry-Paraffin: Ribosomal protein L26 Antibody [NBP1-80812] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine cells.](http://images.novusbio.com/fullsize/RPL26L1-Antibody-Immunohistochemistry-Paraffin-NBP1-80812-img0002.jpg)
![Western Blot: Ribosomal protein L26 Antibody [NB100-2131] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Affinity purified rabbit anti-RPL26 antibody (NB100-2131) used for WB at 0.04 ug/ml (A) and 1 ug/ml (B), and used for IP at 3 ug/mg lysate.](http://images.novusbio.com/fullsize/Ribosomal-protein-L26-Antibody-Western-Blot-NB100-2131-img0003.jpg "Western Blot: Ribosomal protein L26 Antibody [NB100-2131] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Affinity purified rabbit anti-RPL26 antibody (NB100-2131) used for WB at 0.04 ug/ml (A) and 1 ug/ml (B), and used for IP at 3 ug/mg lysate.")
![Immunohistochemistry-Paraffin: Ribosomal protein L26 Antibody [NB100-2131] - Sample: FFPE section of human skin carcinoma. Antibody: Affinity purified rabbit anti-expndtw-4 RPL26 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Ribosomal-protein-L26-Antibody-Immunohistochemistry-Paraffin-NB100-2131-img0007.jpg)
![Western Blot: Ribosomal protein L26 Antibody [NBP2-22233] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. Antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Ribosomal-protein-L26-Antibody-Western-Blot-NBP2-22233-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Ribosomal protein L26 Antibody [NBP2-22233] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. Antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: Ribosomal protein L26 Antibody [NBP2-20161] - Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Ribosomal-protein-L26-Antibody-Western-Blot-NBP2-20161-img0001.jpg "Western Blot: Ribosomal protein L26 Antibody [NBP2-20161] - Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: Ribosomal protein L26 Overexpression Lysate (Adult Normal) [NBL1-15517] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Ribosomal protein L26.](http://images.novusbio.com/fullsize/Ribosomal-protein-L26-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15517-img0002.jpg "Western Blot: Ribosomal protein L26 Overexpression Lysate (Adult Normal) [NBL1-15517] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Ribosomal protein L26.")
![SDS-Page: ribosomal protein L26 Protein [NBP2-23421]](http://images.novusbio.com/fullsize/ribosomal-protein-L26-Protein-SDS-Page-NBP2-23421-img0001.jpg "SDS-Page: ribosomal protein L26 Protein [NBP2-23421]")